Comparison of the Physical Properties and in vivo Bioactivities of Flatwise-Spun Silk Mats and Cocoon-Derived Silk Mats for Guided Bone Regeneration

Kang, Yei-Jin; Jo, You‐Young; Kweon, HaeYong; Chae, Weon‐Sik; Yang, Won-Geun; Garagiola, U.; Kim, Seong‐Gon; Rotaru, Horaţiu

doi:10.1007/s13233-020-8026-z

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…Here, it was hypothesized that during the flow process, RSF molecules transformed from a random coil or helix to an oriented conformation of β-sheet structure, which is consistent with previous reports. [50,56] Moreover, relatively homogeneous RSF/CNF suspensions were obtained due to the shear and elongation, leading to larger order parameter (0.36) and orientation degree. This phenomenon is consistent with Jin's work, where RSF molecules in the solution gradually formed rod-like liquid crystal structures after applying sufficient shear.…”

Section: Resultsmentioning

confidence: 99%

Flow Analysis of Regenerated Silk Fibroin/Cellulose Nanofiber Suspensions via a Bioinspired Microfluidic Chip

Lü

Fan

Geng

et al. 2021

Adv Materials Technologies

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The outstanding performance of silk, especially in terms of mechanical properties, can be attributed to the sophisticated silk gland and specialized spinning method that exists in nature. In addition, the gland, with decreasing diameter, has been proven to be an outstanding actuator of the sophisticated spinning system. [5,9] It has been qualitatively demonstrated that a region of contracting geometry of the S-shaped duct of silk gland can induce stretching of protein molecules, phase separation, and self-assembly due to shear and elongation flow. [10][11][12][13][14][15] During the subsequent spinning process in air, the protein ultimately aggregates into fibers with spectacular and distinctive properties. However, the mechanisms within the glands with exponential decaying diameter and their ability to dynamically change the conformation of the proteins is yet to be elucidated. [6] At present, various biomimetic spinning methods have been used to fabricate high-performance artificial silk and reveal the relationship between the spinning channel and silk performance. For example, the microfluidic device with miniaturized channels of designed configurations can easily control the physicochemical microenvironment, while using trace amounts of reagents. [6,10,[16][17][18][19][20] The sophisticated, compact, and highly functional microfluidic devices have been widely used to understand the geometry confinements, control overflow, and synthesis of artificially regenerated silk fibroin (RSF) fibers. [21][22][23][24][25][26][27][28][29][30] For example, Rammensee et al. prepared assembled spider dragline silk fibers through a microfluidic device. [31] Kinahan et al. adopted the same method to fabricate functional fibers with expected properties by controlling flow conditions. [32] Based on these studies, Zhang's group designed microfluidic chips mimicking the first-order and second-order exponential decay function of silk glands and prepared high-performance artificial silk fibers. [24][25][26]33,34] In our previous work, [35,36] statistics of spider's major ampullate (MA) gland of Nephila edulis were extracted from the work of Knight et al., [12] and then a microfluidic chip was prepared which fitted well to the second-order exponential decay function. The bioinspired chip possessed excellent similarity with the MA gland of spider, and was used to prepare RSF/cellulose nanofibers (CNF) hybrid fibers. However, there were some differences between the size of our microfluidic chip and the ampullate gland of spider. First, the actual diameter of lumen in the MA gland of N. edulis is from ≈250 to 8 µm. [12] However, high concentration of RSF/CNF suspensions (30 wt%) cannot flow through Microfluidic spinning has been used to mimic and discover the natural spinning process of silk. However, it is still challenging to understand the orientation and alignment of silk-spinning through microfluidic chips. Here, flow analysis is performed for a bioinspired microfluidic chip mimicking the shape of a spider's major ampullate gland a...

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Section: Resultsmentioning

confidence: 99%

Flow Analysis of Regenerated Silk Fibroin/Cellulose Nanofiber Suspensions via a Bioinspired Microfluidic Chip

Lü

Fan

Geng

et al. 2021

Adv Materials Technologies

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“…Bone apposition was observed in the proximity of ABXs particles, but resorptive processes were absent. Additionally, a significantly higher amount of NFB was detected in the apical rather than the coronal region of the extraction site, regardless of the grafting procedure, suggesting that bone formation could be initiated from the apical/lateral region of the alveolar socket and was not enhanced from the coronal direction [ 55 , 89 ]. These results are in line with evidence previously reported by Carmagnola and colleagues [ 52 ], who demonstrated that anorganic bone grafting led to less new bone formation and more residual connective tissue compared with cases where graft materials were not used, although no statistical analysis was performed to prove significant differences.…”

Section: Resultsmentioning

confidence: 99%

A comparison between anorganic bone and collagen-preserving bone xenografts for alveolar ridge preservation: systematic review and future perspectives

Stefano

Orlando

Ottobelli³

et al. 2022

Maxillofac Plast Reconstr Surg

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After tooth extraction, dimensional changes affect the alveolar socket, leading to loss in alveolar bone height and width. Histological modifications also occur, with initial formation of a blood clot that is replaced with granulation tissue and subsequently with a provisional connective tissue matrix. Spontaneous healing ends with socket filling with woven bone, which is gradually replaced with lamellar bone and bone marrow. Adequate alveolar ridge dimensions and bone quality are required to assure optimal stability and osseointegration following dental implant placement. When a tooth is extracted, alveolar ridge preservation (ARP) procedures are an effective method to prevent collapse of the post-extraction socket. Heterologous bone is widely chosen by clinicians for ARP, and anorganic bone xenografts (ABXs) made bioinert by heat treatment represents the most used biomaterial in clinical applications. Collagen-preserving bone xenografts (CBXs) made of porcine or equine bone are fabricated by less invasive chemical or enzymatic treatments to remove xenogenic antigens, and these are also effective in preserving post-extraction sites. Clinical differences between anorganic bone substitutes and collagen-preserving materials are not well documented in the literature but understanding these differences could clarify how processing protocols influence biomaterial behavior in situ. This systematic review of the literature compares the dimensional changes and histological features of ABXs versus CBXs in ridge preservation procedures to promote awareness of different bone xenograft efficacies in stimulating the healing of post-extraction sockets.

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Silk sericin application increases bone morphogenic protein-2/4 expression via a toll-like receptor-mediated pathway

Kweon

Kim

et al. 2021

International Journal of Biological Macromolecules

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Comparison of the Physical Properties and in vivo Bioactivities of Flatwise-Spun Silk Mats and Cocoon-Derived Silk Mats for Guided Bone Regeneration

Cited by 5 publications

References 24 publications

Flow Analysis of Regenerated Silk Fibroin/Cellulose Nanofiber Suspensions via a Bioinspired Microfluidic Chip

Flow Analysis of Regenerated Silk Fibroin/Cellulose Nanofiber Suspensions via a Bioinspired Microfluidic Chip

A comparison between anorganic bone and collagen-preserving bone xenografts for alveolar ridge preservation: systematic review and future perspectives

Silk sericin application increases bone morphogenic protein-2/4 expression via a toll-like receptor-mediated pathway

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