Comparison of the PCR with the Cefoxitin Disc Diffusion Test for Detection of Methicillin Resistance in Oxacillin Resistant Coagulase-Negative Staphylococci (Cons)

Kılıç, İbrahim Halil; Özaslan, Mehmet; Zer, Yasemin; Karagöz, Işık Didem; Menteş, Ozlem; Cengiz, Beyhan; Balcı, İclal

doi:10.2478/v10133-010-0045-z

Cited by 1 publication

(1 citation statement)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have shown mostly high sensitivity of PCR up to 97.3% compared to the disk diffusion method [12], Some other studies shows 70.3% [23], While other study gave 59.2% for oxacillin resistant MRSA and 55.2% for cefoxitin resistant MRSA [22,24]. In this study the sensitivity was 55% indicating that the methicillin resistant in S. aureus may not come from mecA gene, it may come from over production of β-lactamase by other gene which have mutation in its regulatory gene, including various length sequences deletion, or conjugation and transformation occur between bacterial cells causing constitutive expression for the β-lactamase [15].…”

Section: Resultsmentioning

confidence: 99%

Detection and Evaluation of Methicillin-Resistant Staphylococcus aureus by Duplex PCR

Kareem

2013

JNUS

View full text Add to dashboard Cite

Staphylococcusaureusis avirulence pathogenic bacterium.Detection of methicillin-resistant S. aureus(MRSA) using conventional culture and biochemical methods is labor and time consuming. Many MRSA isolates are heterogeneously resistant to ß-lactams, for example only 1 daughter cell out of 10 4 to 10 6 cells appears phenotypically resistant when routine antimicrobial susceptibility tests are performed. In this work two genes were used for detection of MRSA in urine, boil and nasal swabs using conventional PCR, mecA gene (533bp) were used for detection of methicillin resistant and femA gene (318bp) were used for S.aureus identification. It was found that not all resistant S. aureus tested with disk diffusion method carry the mecA gene that caused the resistant phenomena there were only 55% of all MRSA carrying mecA gene while femA gene gave 100% positive for S. aureus and this will lead us to appoint that penicillin-bindingprotein 2a (PBP2a) produced by mecA gene is not the only cause of resistant phenomena.

show abstract

Section: Resultsmentioning

confidence: 99%

Detection and Evaluation of Methicillin-Resistant Staphylococcus aureus by Duplex PCR

Kareem

2013

JNUS

View full text Add to dashboard Cite

show abstract

Comparison of the PCR with the Cefoxitin Disc Diffusion Test for Detection of Methicillin Resistance in Oxacillin Resistant Coagulase-Negative Staphylococci (Cons)

Cited by 1 publication

References 30 publications

Detection and Evaluation of Methicillin-Resistant Staphylococcus aureus by Duplex PCR

Detection and Evaluation of Methicillin-Resistant Staphylococcus aureus by Duplex PCR

Contact Info

Product

Resources

About