Comparison of the osteogenic potential of equine mesenchymal stem cells from bone marrow, adipose tissue, umbilical cord blood, and umbilical cord tissue

Abstract

eMSCs from BM possessed the highest in vitro osteogenic potential; eMSCs from adipose tissue also had robust osteogenic potential. The tuber coxae and the sternum were viable sources of BM-eMSCs in yearlings, and 60 mL of BM aspirate was sufficient for culture and expansion. Expanding BM-eMSCs in AS to avoid potential immunologic reactions decreased the total yield because BM-eMSCs grew significantly slower in AS than in fetal bovine serum. Additional studies are needed to determine optimal ex vivo eMSC culture and expansion conditions, including the timing and use of growth factor—supplemented AS.

Comparative immunophenotyping of equine multipotent mesenchymal stromal cells: An approach toward a standardized definition

“…First of all, equine MSCs from different sources were Original Article shown to display differences in growth kinetics (36,46), differentiation potential (36,47), and gene expression (36), thus differences in surface marker expression could be expected as well. Furthermore, differences in the culture medium could influence the cell characteristics (48,49).…”

The Regenerative Medicine Laboratory: Facilitating Stem Cell Therapy for Equine Disease

“…MSCs can be harvested from numerous tissues and locations and may have similar surface markers and growth characteristics, however these MSCs display distinct differences [19]. Some studies, including our own published studies [20], suggest that BMMSCs and adipose-derived MSCs (ASCs) are more closely related than to MSCs derived from placental tissues [20,21]. In humans, cord blood derived-MSCs express genes involved in the cell cycle and in neurogenesis, consistent with their reported neuronal differentiation capacity; BM-MSCs appear to be primed towards developmental processes of tissues and organs derived from the mesoderm and endoderm; and ASCs are highly enriched in immune-related genes [21].…”

In Vitro Studies of Horse Umbilical Cord Matrix-Derived Cells: From Characterization to Labeling for Magnetic Resonance Imaging

“…Specific pluripotent and MSCmarkers of equine and swine UCM are reported in Table 1. Due to their primitive embryonic stem-cell-like characteristics, UCM-derived cells, when exposed to specific stimuli, have the ability to differentiate into multiple germ layers including mesoderm in horses [9,10,13] and ectoderm in pigs [8,14] and horses [9,11]. Others: SOX2+ [9][10][11][12] Swine Embryonic cell markers: OCT-3/4+ Others: SOX2+, NANOG+ [8] As in human medicine (for review see [7,15,16]), also in veterinary medicine an important property to evaluate stem cells is to assess their usefulness in allogenic regenerative medicine.…”