Comparison of the novel ResPlex III assay and existing techniques for the detection and subtyping of influenza virus during the influenza season 2006–2007

Eggers, Maren; Roth, Bernhard; Schweiger, Brunhilde; Schmid, Matthias; Gregersen, Jens‐Peter; Enders, Martin

doi:10.1007/s10096-011-1437-1

Cited by 8 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, limited information is available about the sensitivity and specificity of the ResPlex II v 2.0 test kit. We have compared the novel ResPlex III assay and existing techniques for the detection and subtyping of influenza virus during the influenza season 2006-2007 [27]. The methodology must necessarily make some compromises, for example, with regard to amplification conditions during the first cycles with specific primers.…”

Section: Discussionmentioning

confidence: 99%

Isolation of influenza viruses in MDCK 33016PF cells and clearance of contaminating respiratory viruses

Roth

Mohr

Enders

et al. 2012

Vaccine

View full text Add to dashboard Cite

This paper summarizes results obtained by multiplex PCR screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in MDCK 33016PF cells. Using the ResPlexII v2.0 (Qiagen) multiplex PCR, 393 positive results were obtained in 468 clinical samples collected during an influenza season in Germany. The overall distribution of positive results was influenza A 42.0%, influenza B 38.7%, adenovirus 1.5%, bocavirus 0.5%, coronavirus 3.3%, enterovirus 5.6%, metapneumovirus 1.0%, parainfluenza virus 0.8%, rhinovirus 4.1%, and respiratory syncytial virus (RSV) 2.5%. Double infections of influenza virus together with another virus were found for adenovirus B and E, bocavirus, coronavirus, enterovirus and for rhinovirus. These other viruses were rapidly lost upon passages in MDCK 33016PF cells and under conditions as applied to influenza virus passaging. Clinical samples, in which no influenza virus but other viruses were found, were also subject to passages in MDCK 33016PF cells. Using lower inoculum dilutions than those normally applied for preparations containing influenza virus (total dilution of the original sample of ∼10(4)), the positive results for the different viruses turned negative already after 2 or 3 passages in MDCK 33016PF cells. These results demonstrate that, under practical conditions as applied to grow influenza viruses, contaminating viruses can be effectively removed by passages in MDCK cells. In combination with their superior isolation efficiency, MDCK cells appear highly suitable to be used as an alternative to embryonated eggs to isolate and propagate influenza vaccine candidate viruses.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation of influenza viruses in MDCK 33016PF cells and clearance of contaminating respiratory viruses

Roth

Mohr

Enders

et al. 2012

Vaccine

View full text Add to dashboard Cite

show abstract

“…Infections caused by other respiratory pathogens may present similarly to influenza infections, making it difficult to distinguish them clinically [ 4 ]. Hence, accurate detection and subtyping of influenza virus are important for epidemiologic surveillance [ 5 ], while aiding infection control and management for the individual and public health responses to influenza outbreaks and pandemics [ 6 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

Surveillance and Clinical Characterization of Influenza in a University Cohort in Singapore

et al. 2015

View full text Add to dashboard Cite

BackgroundSoutheast Asia is a potential locus for the emergence of novel influenza strains. However, information on influenza within the region is limited.ObjectivesThis study was to determine the proportion of influenza-like illness (ILI) caused by influenza A and B viruses in a university cohort in Singapore, identify important distinctive clinical features of influenza infection and potential factors associated with influenza infection compared with other causes of ILI.MethodologyA surveillance study was conducted from 2007 to 2009, at the University Health and Wellness Centre, National University of Singapore (NUS). Basic demographic information and nasopharyngeal swabs were collected from consenting students and staff with ILI, with Influenza A and B identified by both culture and molecular methods.ResultsProportions of influenza A and B virus infections in subjects with ILI were 153/500 (30.6%) and 11/500 (2.2%) respectively. The predominant subtype was A/H1N1, including both the seasonal strain (20/153) and the pandemic strain (72/153). The clinical symptom of fever was more common in subjects with laboratory confirmed influenza than other ILIs. On-campus hostel residence and being a student (compared with staff) were associated with increased risk of laboratory confirmed influenza A/H1N1 2009 infection.ConclusionsThis study provides a baseline prevalence of influenza infection within young adults in Singapore in a university setting. Potential risk factors, such as hostel residence, were identified, allowing for more targeted infection control measures in the event of a future influenza pandemic.

show abstract

“…Influenza viruses are evolutionary dynamic viruses with high mutation rate [1] . Accurate detection and further subtyping of influenza A viruses is important for epidemiologic surveillance [2] . Many respiratory pathogens can present with “influenza-like” symptoms.…”

Section: Introductionmentioning

confidence: 99%

Prospective Surveillance and Molecular Characterization of Seasonal Influenza in a University Cohort in Singapore

et al. 2014

View full text Add to dashboard Cite

BackgroundSoutheast Asia is believed to be a potential locus for the emergence of novel influenza strains, and therefore accurate sentinel surveillance in the region is critical. Limited information exists on sentinel surveillance of influenza-like illness (ILI) in young adults in Singapore in a University campus setting. The objective of the present study was to determine the proportion of ILI caused by influenza A and B viruses in a university cohort in Singapore.Methodology/Principal FindingsWe conducted a prospective surveillance study from May through October 2007, at the National University of Singapore (NUS). Basic demographic information and nasopharyngeal swabs were collected from students and staff with ILI. Reverse-transcriptase PCR (RT-PCR) and viral isolation were employed to detect influenza viruses. Sequencing of hemagglutinin (HA) and neuraminidase (NA) genes of some representative isolates was also performed. Overall proportions of influenza A and B virus infections were 47/266 (18%) and 9/266 (3%) respectively. The predominant subtype was A/H3N2 (55%) and the rest were A/H1N1 (45%). The overall sensitivity difference for detection of influenza A viruses using RT-PCR and viral isolation was 53%. Phylogenetic analyses of HA and NA gene sequences of Singapore strains showed identities higher than 98% within both the genes. The strains were more similar to strains included in the WHO vaccine recommendation for the following year (2008). Genetic markers of oseltamivir resistance were not detected in any of the sequenced Singapore isolates.Conclusions/SignificanceHA and NA gene sequences of Singapore strains were similar to vaccine strains for the upcoming influenza season. No drug resistance was found. Sentinel surveillance on university campuses should make use of molecular methods to better detect emerging and re-emerging influenza viral threats.

show abstract

Comparison of the novel ResPlex III assay and existing techniques for the detection and subtyping of influenza virus during the influenza season 2006–2007

Cited by 8 publications

References 18 publications

Isolation of influenza viruses in MDCK 33016PF cells and clearance of contaminating respiratory viruses

Isolation of influenza viruses in MDCK 33016PF cells and clearance of contaminating respiratory viruses

Surveillance and Clinical Characterization of Influenza in a University Cohort in Singapore

Prospective Surveillance and Molecular Characterization of Seasonal Influenza in a University Cohort in Singapore

Contact Info

Product

Resources

About