Comparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis

Öztürk, Emel; Manzano-Román, Raúl; Sánchez-Ovejero, Carlos; Caner, Ayşe; Angın, Mesude; Gündüz, Cumhur; Karaman, Ülkü; Altıntaş, Nuray; Bozkaya, Halil; Ünalp, Ömer Vedat; Dökümcü, Zafer; Divarcı, Emre; Casulli, Adriano; Altıntaş, Nazmiye; Siles‐Lucas, Mar; Ünver, Ayşegül

doi:10.1016/j.actatropica.2021.106208

Cited by 2 publications

(3 citation statements)

References 44 publications

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“…This may be due to a low immunogenicity of these peptides, but technical issues such as a low adsorption onto the ELISA plate due to their intrinsic chemical characteristics may have also occurred. The use of single peptides may also be suboptimal, since it has been suggested that the use a pool of peptides, as opposed to single peptides, might reach higher diagnostic accuracy [ 68 ]. Therefore, future studies should focus on the use of combinations of those peptides with predicted good and complementary accuracy for the diagnosis of CE (vs controls) or of active (vs inactive) CE cysts.…”

Section: Discussionmentioning

confidence: 99%

Antigen discovery by bioinformatics analysis and peptide microarray for the diagnosis of cystic echinococcosis

et al. 2023

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Background Cystic echinococcosis (CE), caused by Echinococcus granulosus sensu lato, is a neglected zoonosis. Its diagnosis relies on imaging, supported by serology, while only imaging is useful for staging and follow-up. Since diagnostic tools and expertise are not widely available, new accurate and easily implementable assays for the diagnosis and follow-up of CE are highly needed. Methodology/Principal Findings We aimed to identify new E. granulosus antigens through a bioinformatics selection applied to the parasite genome, followed by peptide microarray screening and validation in ELISA, using independent panels of sera from patients with hepatic CE and clinically relevant controls. From 950 proteins selected in silico, 2,379 peptides were evaluated by microarray for IgG reactivity and eight candidates selected for validation. Reactivity to one peptide was significantly higher in the CE group (p = 0.044), but had suboptimal diagnostic accuracy. Conclusions/Significance Here we performed bioinformatics analysis and peptide microarray for antigen discovery, useful for the diagnosis of CE. Eight candidates were selected and validated. Reactivity to one peptide associated to CE but had suboptimal diagnostic accuracy. Importantly, the database developed in this study may be used to identify other antigenic candidates for CE diagnosis and follow-up.

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Section: Discussionmentioning

confidence: 99%

Antigen discovery by bioinformatics analysis and peptide microarray for the diagnosis of cystic echinococcosis

et al. 2023

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“…coli Rosetta cells Indirect ELISA 70 lymphatic filariasis-positive serum samples/176 serum samples from healthy individuals/18 serum samples from non-lymphatic filariasis diseases Sensitivity: 100% Specificity: 98.1% and 99.52% [ 126 ]/India Human cystic echinococcosis/ Echinococcus granulosus rMEP E. coli BL21(DE3) cells ELISA 43 echinococcosis-positive serum samples/120 serum samples from healthy individuals/23 serum samples from non-echinococcosis diseases Sensitivity: 95.3% Specificity: 95.0% [ 127 ]/Iran Human cystic echinococcosis/ Echinococcus granulosus DIPOL E . coli cells ELISA 149 echinococcosis-positive serum samples/21 serum samples from healthy individuals/49 serum samples from non-echinococcosis diseases Sensitivity: 75.4% for active and transitional cysts and 95.6% for inactive cysts Specificity: 97.71% [ 128 ]/Turkey Human onchocerciasis/ Onchocerca volvulus OvMCBL02 – Indirect ELISA 63 onchocerciasis-positive serum samples/55 serum sample from healthy individuals/21 serum samples from non-onchocerciasis diseases / 54 onchocerciasis-negative serum samples from ivermectin treated individuals Sensitivity: 98.4% Specificity: 100% [ 129 ]/Belgium “–”: information not provided by the study …”

Section: Rmps Applied In Disease Diagnosismentioning

confidence: 99%

“…However, the Euroimmun commercial kit showed better performance, with 100% for both sensitivity and specificity. More recently, Ozturk et al (2022) [128], also working on diagnosing human cystic echinococcosis, tested an RMP, named as DIPOL. In their study, the authors obtained DIPOL through expression in E. coli cells and performed an ELISA assay to verify antigen reactivity.…”

Section: Rmps Applied In the Diagnosis Of Diseases Caused By Wormsmentioning

confidence: 99%

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

Gonçalves,

Ribeiro,

Resende

et al. 2024

Microb Cell Fact

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Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnosis, as well as discuss the main points surrounding these antigens. RMPs usually consisting of linear, immunodominant, and phylogenetically conserved epitopes, has been applied in the experimental diagnosis of various human and animal diseases, such as leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis, leptospirosis, leprosy, filariasis, schistosomiasis, dengue, and COVID-19. The synthetic genes for these epitopes are joined to code a single RMP, either with spacers or fused, with different biochemical properties. The epitopes’ high density within the RMPs contributes to a high degree of sensitivity and specificity. The RMPs can also sidestep the need for multiple peptide synthesis or multiple recombinant proteins, reducing costs and enhancing the standardization conditions for immunoassays. Methods such as bioinformatics and circular dichroism have been widely applied in the development of new RMPs, helping to guide their construction and better understand their structure. Several RMPs have been expressed, mainly using the Escherichia coli expression system, highlighting the importance of these cells in the biotechnological field. In fact, technological advances in this area, offering a wide range of different strains to be used, make these cells the most widely used expression platform. RMPs have been experimentally used to diagnose a broad range of illnesses in the laboratory, suggesting they could also be useful for accurate diagnoses commercially. On this point, the RMP method offers a tempting substitute for the production of promising antigens used to assemble commercial diagnostic kits.

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Comparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis

Cited by 2 publications

References 44 publications

Antigen discovery by bioinformatics analysis and peptide microarray for the diagnosis of cystic echinococcosis

Antigen discovery by bioinformatics analysis and peptide microarray for the diagnosis of cystic echinococcosis

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis

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