Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci

Kim, Miyoung; Heo, Se Ran; Choi, Sanghyuk; Kwon, Hyelin; Park, Jeong Su; Seong, Moon Woo; Lee, Dong Hoon; Park, Kyoung Un; Song, Jae-Joon; Kim, Eui Chong

doi:10.1186/1471-2180-8-233

Cited by 46 publications

(44 citation statements)

References 25 publications

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“…Although many phenotypic identification methods are commercially available, diagnostic accuracy has been reported to be 36.7 to 93.6% (5,16,19,26). This inaccuracy is problematic in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

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Rapid and Accurate Identification of Human-Associated Staphylococci by Use of Multiplex PCR

et al. 2011

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Although staphylococci are identified by phenotypic analysis in many clinical laboratories, these results are often incorrect because of phenotypic variation. Genetic analysis is necessary for definitive species identification. In the present study, we developed a simple multiplex-PCR (M-PCR) for species identification of human-associated staphylococci, which were as follows: Staphylococcus aureus, S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, S. saprophyticus, and S. warneri. This method was designed on the basis of nucleotide sequences of the thermonuclease (nuc) genes that were universally conserved in staphylococci except the S. sciuri group and showed moderate sequence diversity. In order to validate this assay, 361 staphylococcal strains were studied, which had been identified at the species levels by sequence analysis of the hsp60 genes. In consequence, M-PCR demonstrated a sensitivity of 100% and a specificity of 100%. By virtue of simplicity and accuracy, this method will be useful in clinical research.

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Section: Discussionmentioning

confidence: 99%

“…Although conventional biochemical assays are employed in many clinical laboratories, they are frequently imprecise due to phenotypic variation (5,16,19,26). Real-time PCR has been developed, but some staphylococcal species are indistinguishable, and/or interpretation of results is intricate (12,37).…”

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Rapid and Accurate Identification of Human-Associated Staphylococci by Use of Multiplex PCR

et al. 2011

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“…These systems allow for more rapid and more accurate identification than does manual morphological identification or biochemical tests. However, the accuracy of these tests can be compromised because of the variable expression of phenotypic characteristics and the limited nature of the databases; these limitations can result in ambiguous findings and the inability to identify uncommon isolates (4,15,17). In addition, identification of CNS to the species level may change the diagnosis and therapeutic plans, since uncommon isolates are being considered as causative agents, and unusual antibacterial resistance patterns appear (1,13).…”

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confidence: 99%

tuf Gene Sequence Analysis Has Greater Discriminatory Power than 16S rRNA Sequence Analysis in Identification of Clinical Isolates of Coagulase-Negative Staphylococci

et al. 2011

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We compared and analyzed 16S rRNA and tuf gene sequences for 97 clinical isolates of coagulase-negative staphylococci (CNS) by use of the GenBank, MicroSeq, EzTaxon, and BIBI databases. Discordant results for definitive identification were observed and differed according to the different databases and target genes. Although higher percentages of sequence identity were obtained with GenBank and MicroSeq for 16S rRNA analysis, the BIBI and EzTaxon databases produced less ambiguous results. Greater discriminatory power and fewer multiple probable identifications were observed with tuf gene analysis than with 16S rRNA analysis. The most pertinent results for tuf gene analysis were obtained with the GenBank database when the cutoff values for the percentage of identity were adjusted to be greater than or equal to 98.0%, with >0.8% separation between species. Analysis of the tuf gene proved to be more discriminative for certain CNS species; further, this method exhibited better distinction in the identification of CNS clinical isolates.

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“…Of the urinary CoNS isolates, S. saprophyticus is identified presumptively by simplified conventional methods and commercially automated identification methods (2)(3)(4)(5)(6)(7)(8)(9). However, misidentification of S. saprophyticus by these simplified conventional and automated phenotypic methods often occurs.…”

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Comparison of the Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with That of Other Commercial Identification Systems for Identifying Staphylococcus saprophyticus in Urine

et al. 2013

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Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci

Cited by 46 publications

References 25 publications

Rapid and Accurate Identification of Human-Associated Staphylococci by Use of Multiplex PCR

Rapid and Accurate Identification of Human-Associated Staphylococci by Use of Multiplex PCR

tuf Gene Sequence Analysis Has Greater Discriminatory Power than 16S rRNA Sequence Analysis in Identification of Clinical Isolates of Coagulase-Negative Staphylococci

Comparison of the Accuracy of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry with That of Other Commercial Identification Systems for Identifying Staphylococcus saprophyticus in Urine

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