Comparison of the microbiome, metabolome, and lipidome of obese and non-obese horses

Coleman, Michelle; Whitfield-Cargile, Canaan; Madrigal, Rodolfo; Cohen, Noah D.

doi:10.1371/journal.pone.0215918

Cited by 26 publications

(19 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have indicated that an altered serum metabolome profile could reflect differences in the gut microbiome of animals [11,16,57], which consistent with our findings (Fig. 7).…”

Section: Discussionsupporting

confidence: 93%

Effect of host breeds on gut microbiome and serum metabolome in meat rabbits

Zhou

Zhang

et al. 2021

BMC Vet Res

View full text Add to dashboard Cite

Background Gut microbial compositional and functional variation can affect health and production performance of farm animals. Analysing metabolites in biological samples provides information on the basic mechanisms that affect the well-being and production traits in farm animals. However, the extent to which host breeds affect the gut microbiome and serum metabolome in meat rabbits is still unknown. In this study, the differences in phylogenetic composition and functional capacities of gut microbiota in two commercial rabbit breeds Elco and Ira were determined by 16S rRNA gene and metagenomic sequencing. The alternations in serum metabolome in the two rabbit breeds were detected using ultra-performance liquid chromatography system coupled with quadrupole time of flight mass spectrometry (UPLC-QTOFMS). Results Sequencing results revealed that there were significant differences in the gut microbiota of the two breeds studied, suggesting that host breeds affect structure and diversity of gut microbiota. Numerous breed-associated microorganisms were identified at different taxonomic levels and most microbial taxa belonged to the families Lachnospiraceae and Ruminococcaceae. In particular, several short-chain fatty acids (SCFAs) producing species including Coprococcus comes, Ruminococcus faecis, Ruminococcus callidus, and Lachnospiraceae bacterium NK4A136 could be considered as biomarkers for improving the health and production performance in meat rabbits. Additionally, gut microbial functional capacities related to bacterial chemotaxis, ABC transporters, and metabolism of different carbohydrates, amino acids, and lipids varied greatly between rabbit breeds. Several fatty acids, amino acids, and organic acids in the serum were identified as breed-associated, where certain metabolites could be regarded as biomarkers correlated with the well-being and production traits of meat rabbits. Correlation analysis between breed-associated microbial species and serum metabolites revealed significant co-variations, indicating the existence of cross-talk among host-gut microbiome-serum metabolome. Conclusions Our study provides insight into how gut microbiome and serum metabolome of meat rabbits are affected by host breeds and uncovers potential biomarkers important for breed improvement of meat rabbits.

show abstract

Section: Discussionsupporting

confidence: 93%

Effect of host breeds on gut microbiome and serum metabolome in meat rabbits

Zhou

Zhang

et al. 2021

BMC Vet Res

View full text Add to dashboard Cite

show abstract

“…Ribose, a metabolite of the pentose phosphate pathway in carbohydrate metabolism, was significantly elevated on Day 3, consistent with an oxidative stress response noted in the metabolomic profiles dogs with gastrointestintal disease [33]. Similarly, decreases in tocopherols, analogs of the anti-oxidant vitamin E, have been reported in horses suffereing from obesity [35].…”

Section: Plos Onesupporting

confidence: 64%

The cecal and fecal microbiomes and metabolomes of horses before and after metronidazole administration

et al. 2020

View full text Add to dashboard Cite

Antibiotic administration can be a cause of gastrointestinal disease in horses, creating a disruption in the normal population and function of bacteria found in the hindgut. The objective of this study was to describe the changes in the cecal and fecal microbiomes and metabolomes of clinically healthy horses before and after metronidazole administration. Metronidazole (15 mg/kg BID PO) was given to five horses with cecal cannulas. The study was suspended on Day 3 due to adverse gastrointestinal effects. Cecal and fecal samples were obtained before (Days minus52, m28, m14, and 0) and after (Days 7, 14, 28, and 52) metronidazole administration. DNA was extracted from the cecal and fecal samples, and 16S rRNA genes were sequenced. Richness and evenness indices were significantly decreased by metronidazole administration in both cecal and fecal samples, but the overall composition was only significantly changed in fecal samples on Day 3 (ANOSIM, p = 0.008). The most dominant phyla were Bacteroidetes and Firmicutes in all groups examined. In fecal samples, significant changes of the phyla Actinobacteria, Spirochaetes, Lentisphaerae, and Verrucomicrobia occurred on Day 3, which correlated with clinical signs of gastrointestinal disease. The metabolome was characterized by mass spectrometry-based methods and only named metabolites were included in the analysis. Fecal, but not cecal, metabolites were significantly affected by metronidazole. The fecal metabolites affected represent diverse metabolic pathways, such as the metabolism of amino acids, carbohydrates, lipids, nucleic acids and cofactors and vitamins. Metronidazole administration has potential to cause adverse effects in horses, alters the bacterial composition of the horse's cecal and fecal content, and the metabolome of fecal samples.

show abstract

“…Further, an impact of the high NSC diet on the microbiome, itself affecting the metabolome, cannot be excluded, even if samples were taken at the beginning of the dietary challenge. Indeed, the influence of the microbiome on markers of the glucose/insulin homeostasis has previously been described in horses [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet

Delarocque

Reiche²,

Meier³

et al. 2021

BMC Vet Res

View full text Add to dashboard Cite

Background Insulin dysregulation (ID) is a key risk factor for equine endocrinopathic laminitis, but in many cases ID can only be assessed accurately using dynamic tests. The identification of other biomarkers could provide an alternative or adjunct diagnostic method, to allow early intervention before laminitis develops. The present study characterised the metabolome of ponies with varying degrees of ID using basal and postprandial plasma samples obtained during a previous study, which examined the predictive power of blood insulin levels for the development of laminitis, in ponies fed a high-sugar diet. Samples from 10 pre-laminitic (PL – subsequently developed laminitis) and 10 non-laminitic (NL – did not develop laminitis) ponies were used in a targeted metabolomic assay. Differential concentration and pathway analysis were performed using linear models and global tests. Results Significant changes in the concentration of six glycerophospholipids (adj. P ≤ 0.024) and a global enrichment of the glucose-alanine cycle (adj. P = 0.048) were found to characterise the response of PL ponies to the high-sugar diet. In contrast, the metabolites showed no significant association with the presence or absence of pituitary pars intermedia dysfunction in all ponies. Conclusions The present results suggest that ID and laminitis risk are associated with alterations in the glycerophospholipid and glucose metabolism, which may help understand and explain some molecular processes causing or resulting from these conditions. The prognostic value of the identified biomarkers for laminitis remains to be investigated in further metabolomic trials in horses and ponies.

show abstract

Comparison of the microbiome, metabolome, and lipidome of obese and non-obese horses

Cited by 26 publications

References 70 publications

Effect of host breeds on gut microbiome and serum metabolome in meat rabbits

Effect of host breeds on gut microbiome and serum metabolome in meat rabbits

The cecal and fecal microbiomes and metabolomes of horses before and after metronidazole administration

Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet

Contact Info

Product

Resources

About