Comparison of the metabolism of 7-ethoxycoumarin and coumarin in precision-cut rat liver and lung slices

Price, Roger J.; Renwick, A.B.; Beamand, J.A.; Esclangon, F; Wield, P.T.; Walters, D. Gareth; Lake, Brian G.

doi:10.1016/0278-6915(94)00129-c

Cited by 30 publications

(14 citation statements)

References 24 publications

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“…In contrast to the present study, others [4,7] have routinely included glucocorticoids in their incubations. We therefore investigated whether glucocorticoids influence MCh-induced bronchoconstriction in lung slices.…”

Section: Steroidsmentioning

confidence: 88%

“…For the lung, an agarose gel instilling technique was developed that enables preparation of tissue cores of this relatively soft tissue [3]. Slices prepared from rat, mouse and human lung were successfully cultured for 48 h [4][5][6]. Although precision-cut lung slices have been successfully utilized in toxicological studies [4,5], their use in pharmacological or functional studies has not been reported.…”

mentioning

confidence: 99%

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Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices

Martin¹,

Uhlig²,

Ullrich³

1996

Eur Respir J

150

187

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V Vi id de eo om mi ic cr ro os sc co op py y o of f m me et th ha ac ch ho ol li in ne e--i in nd du uc ce ed d c co on nt tr ra ac ct ti io on n o of f i in nd di iv vi id d--u ua al l a ai ir rw wa ay ys s i in n p pr re ec ci is si io on n--c cu ut t l lu un ng g s sl li ic ce es s Lung slices of 250±20 µm were prepared from Wistar rats and cultured in a roller incubator in serum-free minimum essential medium (MEM). Under these conditions, the slices were viable for at least 70 h, as indicated by leakage of lactate dehydrogenase into the supernatant, thymidine incorporation and ciliary beating. The slices were placed in a newly developed incubation chamber and mounted by a nylon thread that was fixed to a platinum wire. The whole chamber was positioned on a microscope stage, and contraction of single airways was followed under a microscope that was coupled to a CCD-camera. Reduction in airway area was taken as an index of bronchoconstriction and was determined by a computer program.Addition of methacholine resulted in a concentration-dependent (concentration producing half the maximal effect (EC50)=0.64±0.08 (mean±SD) µM; n=64) contraction of single airways. In the presence of hydrocortisone, the EC50 was about six times greater, i.e. 3.7±0.9 µM (n=7), and the effect of the steroid was largely abolished by propanolol (EC50=1.1±0.1 µM; n=7). Airways with an area smaller than 35,000 µm 2 were nearly nine times more sensitive to methacholine (EC50=0.1±0.03 µM; n=20) than larger ones (EC50=87±0.27 µM; n=22).We conclude that cultured precision-cut lung slices are a useful model for routine study of contraction of individual airways of various sizes. The measurements were precise and reproducible and showed that smaller airways are more sensitive to methacholine than larger ones.

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Section: Steroidsmentioning

confidence: 88%

mentioning

confidence: 99%

Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices

Martin¹,

Uhlig²,

Ullrich³

1996

Eur Respir J

150

187

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“…Evaluation of the metabolism of xenobiotics, including pharmaceuticals [14,23,24,33], continues to be a primary use of the lungslice approach. The respiratory tract is an important site for the detoxification or metabolic activation of many chemicals (e.g., via cytochrome P450 enzymes) [34].…”

Section: Applicationsmentioning

confidence: 99%

“…The gentle rolling motion of the vials allows the tissue slice to be exposed on both sides to air or the liquid medium, thus providing exposure to xenobiotics through the liquid or gaseous phase. This approach has been used to study cyclosporine and its derivatives [23], 7-ethoxycoumarin and paraquat [24,25], complex aerosols [26], benzo[a] pyrene [13] and jet fuel [27]. Tissues from several different species, including humans, have been studied in this manner [6].…”

Section: Reviewmentioning

confidence: 99%

In vitrolung slices: a powerful approach for assessment of lung pathophysiology

Liberati

Randle

Toth

2010

Expert Review of Molecular Diagnostics

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This review summarizes the existing literature on the use of in vitro lung slices to study pulmonary physiology, pharmacology, pathogenesis and toxicity. Since in vitro lung slices maintain cell-cell and cell-matrix relationships in a highly controllable and accessible setting, they offer many advantages over both in vivo and single-cell culture systems. With the advent of high-production slicers, lung slices can be rapidly and reproducibly generated, including from animals treated in vivo. Slices can then be treated in vitro and analyzed using high-throughput technology. Therefore, the lung-slice system offers broad, current and unrealized potential for the detection of toxicity and the delineation of pathophysiologic and therapeutic mechanisms.

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“…They have been applied in pharmacological and toxicological studies (Fisher & Placke 1987; Fisher et al 1994; Parrish et al 1995; Bach et al 1996; Monteil et al 1999; Catania et al 2007; Sturton et al 2008; Nassimi et al 2009). Xenobiotically induced metabolism, bioactivation and detoxification have also been studied (Parrish et al 1995; Price et al 1995; Price et al 1996; Lake et al 2003; De Kanter et al 2004; Price et al 2004; Harrigan et al 2006; Umachandran & Ioannides 2006; Pushparajah et al 2008; Hanlon et al 2009). Another interesting aspect of precision-cut lung slices is the use of tissue sections for infection studies.…”

Section: Short Review Of the History Of Precision-cut Lung Slicesmentioning

confidence: 99%

Assessment of immunotoxicity using precision-cut tissue slices

Sewald

Braun

2012

Xenobiotica

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1. When the immune system encounters incoming infectious agents, this generally leads to immunity. The evoked immune response is usually robust, but can be severely perturbed by potentially harmful environmental agents such as chemicals, pharmaceuticals and allergens.2. Immunosuppression, hypersensitivity and autoimmunity may occur due to changed immune activity. Evaluation of the immunotoxic potency of agents as part of risk assessment is currently established in vivo with animal models and in vitro with cell lines or primary cells.3. Although in vivo testing is usually the most relevant situation for many agents, more and more in vitro models are being developed for assessment of immunotoxicity. In this context, hypersensitivity and immunosuppression are considered to be a primary focus for developing in vitro methods. Three-dimensional organotypic tissue models are also part of current research in immunotoxicology.4. In recent years, there has been a revival of interest in organotypic tissue models. In the context of immunotoxicity testing, precision-cut lung slices in particular have been intensively studied. Therefore, this review is very much focused on pulmonary immunotoxicology. Respiratory hypersensitivity and inflammation are further highlighted aspects of this review. Immunotoxicity assessment currently is of limited use in other tissue models, which are therefore described only briefly within this review.

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Comparison of the metabolism of 7-ethoxycoumarin and coumarin in precision-cut rat liver and lung slices

Cited by 30 publications

References 24 publications

Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices

Videomicroscopy of methacholine-induced contraction of individual airways in precision-cut lung slices

In vitrolung slices: a powerful approach for assessment of lung pathophysiology

Assessment of immunotoxicity using precision-cut tissue slices

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