Comparison of the Melatonin and Calmodulin in Paravertebral Muscle and Platelets of Patients With or Without Adolescent Idiopathic Scoliosis

Acaroğlu, Emre; Akel, İbrahim; Alanay, Ahmet; Yazici, Muharrem; Marcucio, Ralph

doi:10.1097/brs.0b013e3181a3c7a2

Cited by 46 publications

(50 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The bipedal rat/mouse model has been extensively used for scoliosis research [18,19,38,39]. However, validity of this model has been questioned by Janssen et al [40].…”

Section: Discussionmentioning

confidence: 99%

Selective estrogen receptor modulation prevents scoliotic curve progression: radiologic and histomorphometric study on a bipedal C57Bl6 mice model

et al. 2013

Self Cite

View full text Add to dashboard Cite

Purpose Previous work has suggested that progression of experimental scoliotic curves in pinealectomized chicken and bipedal C57BL6 mice models may be prevented and reversed with Tamoxifen treatment. Raloxifene is another Selective Estrogen Receptor Modulator (SERM) with estrogen agonist effects on bone and increases bone density but with fewer side effects on humans. To investigate whether scoliosis progression in bipedal C57Bl6 mice model could be prevented with SERM treatment and the mechanisms associated with this effect. Methods Eighty C57BL6 mice were rendered bipedal and divided into Tamoxifen (TMX), Raloxifene (RLX) and control groups. TMX and RLX groups received orally administered TMX and RLX for 40 weeks. Anteroposterior X-ray imaging and histomorphometric analysis (at 20th and 40th weeks) were performed. Results At 20th week, TMX and RLX groups displayed higher rates (p = 0.033, p = 0.029) and larger curve magnitudes (p = 0.018). At 40th week, curve rates were similar between the groups but the curve magnitudes in TMX and RLX groups were smaller (p = 0.001). Histomorphometry revealed that treated animals had higher trabecular density (p = 0.04), lower total intervertebral disc (p = 0.038) and growth plate volumes (p = 0.005) and smaller vertebral bodies (p = 0.016). Conclusions Treatment with TMX or RLX did not reduce the incidence of scoliosis but decreased the curve magnitudes at 40 weeks. The underlying mechanism associated with the decrease in curve magnitudes may be the early maturation of growth plates, thereby possible deceleration of the growth rate of the vertebral column and increase in bone density. RLX is as effective as TMX in preventing the progression of scoliotic curves in melatonin deficient bipedal mice.

show abstract

“…The bipedal rat/mouse model has been extensively used for scoliosis research [18,19,38,39]. However, validity of this model has been questioned by Janssen et al [40].…”

Section: Discussionmentioning

confidence: 99%

Selective estrogen receptor modulation prevents scoliotic curve progression: radiologic and histomorphometric study on a bipedal C57Bl6 mice model

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Calmodulin, a calcium-binding receptor protein, regulates contractile properties in platelets and muscles, and interacts with melatonin. Increased levels of calmodulin in platelets and an asymmetrical distribution of calmodulin in paraspinal muscles compared to healthy controls have been described in IS patients [18, 19]. …”

Section: Aetiology and Pathogenesismentioning

confidence: 99%

Genetics and pathogenesis of idiopathic scoliosis

2016

View full text Add to dashboard Cite

Idiopathic scoliosis (IS), the most common spinal deformity, affects otherwise healthy children and adolescents during growth. The aetiology is still unknown, although genetic factors are believed to be important. The present review corroborates the understanding of IS as a complex disease with a polygenic background. Presumably IS can be due to a spectrum of genetic risk variants, ranging from very rare or even private to very common. The most promising candidate genes are highlighted.

show abstract

“…Recently, the possible role of calmodulin (CaM) as a mediator of the melatonin antiscoliosis effects has been proposed [78–80]. Melatonin is an inhibitor of calmodulin [81, 82] and, the loss of this inhibition, due to the lack of melatonin, could be the cause of scoliosis in these animal models.…”

Section: Melatonin and Adolescent Idiopathic Scoliosis (Ais)mentioning

confidence: 99%

“…In a similar study, carried out on C57BL6 mice (which are genetically melatonin deficient), it was observed that they develop scoliosis when rendered bipedal; in these animals as well, tamoxifen improved the scoliosis deformities. In humans, Acaroglu et al [78] compared the content of CaM and melatonin in muscle and platelets of scoliotic and healthy populations. The patients suffering with AIS had asymmetric distribution of CaM in the paraspinal muscles, with its concentration being higher at the convex side and lower at the concave curvatures of the spinal column, whereas neither platelet melatonin nor platelet CaM was found to be representative of the muscle protein values.…”

Section: Melatonin and Adolescent Idiopathic Scoliosis (Ais)mentioning

confidence: 99%

Scientific Basis for the Potential Use of Melatonin in Bone Diseases: Osteoporosis and Adolescent Idiopathic Scoliosis

Sánchez‐Barceló

Mediavilla

Tan

et al. 2010

Journal of Osteoporosis

View full text Add to dashboard Cite

The objective of this paper was to analyze the data supporting the possible role of melatonin on bone metabolism and its repercussion in the etiology and treatment of bone pathologies such as the osteoporosis and the adolescent idiopathic scoliosis (AIS). Melatonin may prevent bone degradation and promote bone formation through mechanisms involving both melatonin receptor-mediated and receptor-independent actions. The three principal mechanisms of melatonin effects on bone function could be: (a) the promotion of the osteoblast differentiation and activity; (b) an increase in the osteoprotegerin expression by osteoblasts, thereby preventing the differentiation of osteoclasts; (c) scavenging of free radicals generated by osteoclast activity and responsible for bone resorption. A variety of in vitro and in vivo experimental studies, although with some controversial results, point toward a possible role of melatonin deficits in the etiology of osteoporosis and AIS and open a new field related to the possible therapeutic use of melatonin in these bone diseases.

show abstract

Comparison of the Melatonin and Calmodulin in Paravertebral Muscle and Platelets of Patients With or Without Adolescent Idiopathic Scoliosis

Abstract: AIS group had an asymmetric distribution of calmodulin in paraspinal muscle, higher at the convex side and lower at the concave. Neither platelet melatonin nor platelet calmodulin was found to be representative of the muscle protein values.

Cited by 46 publications

References 19 publications

Selective estrogen receptor modulation prevents scoliotic curve progression: radiologic and histomorphometric study on a bipedal C57Bl6 mice model

Selective estrogen receptor modulation prevents scoliotic curve progression: radiologic and histomorphometric study on a bipedal C57Bl6 mice model

Genetics and pathogenesis of idiopathic scoliosis

Scientific Basis for the Potential Use of Melatonin in Bone Diseases: Osteoporosis and Adolescent Idiopathic Scoliosis

Contact Info

Product

Resources

About