Comparison of the Mechanical Properties of Early Leukocyte- and Platelet-Rich Fibrin versus PRGF/Endoret Membranes

Abstract

Objectives. The mechanical properties of membranes are important factors in the success of treatment and clinical handling. The goal of this study was to compare the mechanical properties of early leukocyte- and platelet-rich fibrin (L-PRF) versus PRGF/Endoret membrane. Materials and Methods. In this experimental study, membranes were obtained from 10 healthy male volunteers. After obtaining 20 cc venous blood from each volunteer, 10 cc was used to prepare early L-PRF (group 1) and the rest was used to get a membrane by PRGF-Endoret system (group 2). Tensile loads were applied to specimens using universal testing machine. Tensile strength, stiffness, and toughness of the two groups of membranes were calculated and compared by paired t-test. Results. The mean tensile strength and toughness were higher in group 1 with a significant difference (P < 0.05). The mean stiffness in group 1 was also higher but not statistically significant (P > 0.05). Conclusions. The results showed that early L-PRF membranes had stronger mechanical properties than membranes produced by PRGF-Endoret system. Early L-PRF membranes might have easier clinical handling and could be a more proper scaffold in periodontal regenerative procedures. The real results of the current L-PRF should be in fact much higher than what is reported here.

In Vitro Evaluation of Mechanical and Structural Properties of Leukocyte-Platelet-Rich Fibrin and Platelet-Rich Fibrin Matrix

“…Our results are contradictory to the results obtained by Khorshidi et al (20) who compared L-PRF versus PRGF/Endoret membrane. Although calcium chloride is used in PRGF Endoret system, the second centrifugation used in PRFM preparation isn't used.…”

Biofabrication of a novel leukocyte-fibrin-platelet membrane as a cells and growth factors delivery platform for tissue engineering applications

“…A significant increase was observed with regard to the stiffness, but no other biomechanical difference was detected. To the best of our knowledge, the investigation of L‐PRF mechanical properties have been reported by other research studies (Khorshidi et al, ; Madurantakam, Yoganarasimha, & Hasan, ), assessing for their good elasticity and high capacity of deformation. Our results are in agreement with the cited literature with particular regard to the high extensibility of the LFPm.…”

“…Mechanical characteristics of the membrane affect the final results in tissue regeneration and its tensile strength when sutured may influence the clinical outcome of tissue healing. (Khorshidi, Raoofi, Bagheri, & Banihashemi, ). In the light of this, elucidating LFPm physical properties appears to be important.…”

“…To the best of our knowledge, the investigation of L-PRF mechanical properties have been reported by other research studies (Khorshidi et al, 2016;Madurantakam, Yoganarasimha, & Hasan, 2015), assessing for their good elasticity and high capacity of deformation.…”

Regenerative potential of leucocyte‐ and platelet‐rich fibrin. Part B: sinus floor elevation, alveolar ridge preservation and implant therapy. A systematic review

“…After centrifugation, three layers are obtained: at the bottom, red blood corpuscles (RBC); at the top, platelet-poor plasma (PPP); and in the middle, a fibrin clot (L-PRF). L-PRF contains a dense fibrin fibre network where platelets and leucocytes are enmeshed and it can serve as scaffold for other type of cells due to its favourable mechanical properties (Rowe et al 2007, Khorshidi et al 2016. Its content in leucocytes and platelets results in a constant release of growth factors such as PDGF, transforming growth factor (TGF), vascular endothelial growth factor (VEGF) and insulinlike growth factor (IGF) for 7-14 days (Dohan et al 2006b, Sch€ ar et al 2015.…”