Comparison of the Influence of “Solvent/Non-Solvent” Treatment for the Attachment of Signal Molecules on the Structure of Electrospun PCL and PLLA Biodegradable Scaffolds

Abstract

Abstract. Electrospun biodegradable scaffolds (matrixes) made of poly(ε-caprolactone) (PCL) and poly(L-lactic acid) (PLLA) are three-dimensional fibrous structures that are commonly used in regenerative medicine and drug delivery systems. Modification of such structures allows manipulating with biological and immune response. Previously, our team suggested a number of surface modification strategies for thin films made of PLLA. One of the proposed strategies are based on treatment of the material with "solvent/non-solvent" mixture that allows absorbing biologically active molecules or linkers on the surface of the sample. The aim of this work was to compare the influence of "solvent/non-solvent" treatment on the structure and crystallinity of the elecrospun biodegradable PCL and PLLA scaffolds. For that purpose, original PCL and PLLA scaffolds were treated with mixture of toluene and ethanol in different proportions. Morphology of the obtained samples was studied using scanning electron microscopy. It was shown that "solvent/non-solvent" treatment doesn't lead to changes in scaffolds morphology such as gluing or cutting of the matrix fibers. By means of X-ray diffraction analysis it was shown that treatment of the samples with selected mixtures doesn't change material crystallinity. Thus, it was demonstrated that proposed composition of the "solvent/nonsolvent" mixture can be used for the modification of electrospun PCL and PLLA scaffolds.

Long Bone Defect Filling with Bioactive Degradable 3D-Implant: Experimental Study

“…A promising approach for modifying the surface of biodegradable polyesters involves treatment of the polymer with a mixture of organic solvents to partially swell its surface [ 41 , 42 , 43 ]. It was shown that a mixture of toluene and ethanol has no destructive effect on the PCL scaffold structure and allows non-covalent immobilization of gelatin and iodine.…”

A first method for preparation of biodegradable fibrous scaffolds containing iodine on the fibre surfaces

“…Previously, our group proved the possibility of the surface modification of such biodegradable polymers as poly(L-lactic acid) and poly(ε-caprolactone) electrospun-fibrous scaffolds by 'solvent/non-solvent' method [10]. This technique includes treatment of the polymer surface with mixture of solvents followed by exposure into the medium, containing modifying agent [11].…”