Comparison of the Infant and Adult Adipose-Derived Mesenchymal Stem Cells in Proliferation, Senescence, Anti-oxidative Ability and Differentiation Potential

Wu, Szu-Hsien; Yu, Jin-Huei; Liao, Yu-Ting; Liu, Kuo-Hao; Chiang, En-Rung; Chang, Ming-Chau; Wang, Jung‐Pan

doi:10.1007/s13770-022-00431-x

Cited by 17 publications

(16 citation statements)

References 51 publications

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“…Recent studies have shown the participation of cell senescence in the regulation of ADSC differentiation potential. The results of these studies remain unclear: some studies have reported the suppression of all differentiations [ 54 , 55 ], but some studies have demonstrated the enhanced osteogenic potential of senescent ADSCs [ 56 , 57 ]. The senescence-dependent enhancement of osteogenesis seems pathological: the senescence-associated secretory phenotype contains many pro-fibrotic, pro-inflammatory, and immunosuppressive soluble factors including TGFβ, which is a strong inducer of osteogenesis [ 58 , 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Type 2 Diabetes Mellitus Facilitates Shift of Adipose-Derived Stem Cells Ex Vivo Differentiation toward Osteogenesis among Patients with Obesity

Agareva

Stafeev

Michurina

et al. 2022

Life

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Objective: Sedentary behavior with overnutrition provokes the development of obesity, insulin resistance, and type 2 diabetes mellitus (T2DM). The main progenitor cells of adipose tissue are adipose-derived stem cells (ADSCs) which can change differentiation, metabolic, and secretory phenotypes under obesity conditions. The purpose of this study was to evaluate ADSC osteogenesis activity among patients with obesity in normal glucose tolerance (NGT) and T2DM conditions. Methods: In the study, ADSCs from donors with obesity were used. After clinical characterization, all patients underwent bariatric surgery and ADSCs were isolated from subcutaneous fat biopsies. ADSCs were subjected to osteogenic differentiation, stained with Alizarin Red S, and harvested for real-time PCR and Western blotting. Cell senescence was evaluated with a β-galactosidase-activity-based assay. Results: Our results demonstrated the significantly increased calcification of ADSC on day 28 of osteogenesis in the T2DM group. These data were confirmed by the statistically significant enhancement of RUNX2 gene expression, which is a master regulator of osteogenesis. Protein expression analysis showed the increased expression of syndecan 1 and collagen I before and during osteogenesis, respectively. Moreover, T2DM ADSCs demonstrated an increased level of cellular senescence. Conclusion: We suggest that T2DM-associated cellular senescence can cause ADSC differentiation to shift toward osteogenesis, the impaired formation of new fat depots in adipose tissue, and the development of insulin resistance. The balance between ADSC adipo- and osteogenesis commitment is crucial for the determination of the metabolic fate of patients and their adipose tissue.

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Section: Discussionmentioning

confidence: 99%

Type 2 Diabetes Mellitus Facilitates Shift of Adipose-Derived Stem Cells Ex Vivo Differentiation toward Osteogenesis among Patients with Obesity

Agareva

Stafeev

Michurina

et al. 2022

Life

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“…The anti-oxidative stress [ 93 ] and anti-apoptotic [ 94 ] effects of substances secreted by ADSCs are critical for repairing radiation-induced DNA damage in the acute phase and preventing the exacerbation of damage caused by the bystander effect. ADSC can effectively activate the antioxidant system, such as superoxide dismutase (SOD) [ 95 , 96 ], Nrf2-antioxidant response element (ARE) pathway [ 97 – 99 ] and so on.…”

Section: The Therapeutic Value Of Adipose-derived Stem Cells (Adscs) ...mentioning

confidence: 99%

The therapeutic effect of adipose-derived stem cells on soft tissue injury after radiotherapy and their value for breast reconstruction

Tang

Liang

et al. 2022

Stem Cell Res Ther

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Background Postmastectomy radiotherapy is considered to be a necessary treatment in the therapy of breast cancer, while it will cause soft tissue damage and complications, which are closely related to the success rate and effectiveness of breast reconstruction. After radiotherapy, cutaneous tissue becomes thin and brittle, and its compliance decreases. Component fat grafting and adipose-derived stem cell therapy are considered to have great potential in treating radiation damage and improving skin compliance after radiotherapy. Main body In this paper, the basic types and pathological mechanisms of skin and soft tissue damage to breast skin caused by radiation therapy are described. The 2015–2021 studies related to stem cell therapy in PubMed were also reviewed. Studies suggest that adipose-derived stem cells exert their biological effects mainly through cargoes carried in extracellular vesicles and soluble secreted factors. Compared to traditional fat graft breast reconstruction, ADSC therapy amplifies the effects of stem cells in it. In order to obtain a more purposeful therapeutic effect, proper stem cell pretreatment may achieve more ideal and safe results. Conclusion Recent research works about ADSCs and other MSCs mainly focus on curative effects in the acute phase of radiation injury, and there is little research about treatment of chronic phase complications. The efficacy of stem cell therapy on alleviating skin fibrosis and its underlying mechanism require further research.

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“…The donors' age negatively impacts the cell yield, longevity, and differentiative potential. A comparison of MSCs from infants and adults showed that donors' age negatively affected their proliferative capacity, antiaging ability, antioxidant capacity, and part of differentiation potential [42]. The mechanism was further explored by analyzing robust differentially expressed genes (DEGs) [43].…”

Section: Age Of Donorsmentioning

confidence: 99%

Application of telomere biology and telomerase in mesenchymal stem cells

Jing¹,

Zhou²,

Zou³

et al. 2022

Nano TransMed

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With the increasing understanding of mesenchymal stem cells (MSCs), their potential in tissue engineering and regenerative medicine has attracted more attention. However, some important problems need to be solved before clinical application, such as low amplification efficiency, inconsistent cell product quality, and unsatisfactory survival rate at the receptor site. Telomeres act as a clock, and they shorten when cells divide. The main mechanism for reversing telomere length is telomerase. Furthermore, telomerase is involved in antioxidation, antiapoptosis, immunological modulation, and other noncanonical processes in addition to proliferation-related tasks. Therefore, it is necessary to understand the telomere biology and telomerase of MSCs to improve their proliferation, performance stability, and antiscavenging ability. This review summarizes the progress of telomerase biological function and mechanism in MSCs, and discusses the current situation and deficiency of telomerase-related application in MSCs.

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Comparison of the Infant and Adult Adipose-Derived Mesenchymal Stem Cells in Proliferation, Senescence, Anti-oxidative Ability and Differentiation Potential

Cited by 17 publications

References 51 publications

Type 2 Diabetes Mellitus Facilitates Shift of Adipose-Derived Stem Cells Ex Vivo Differentiation toward Osteogenesis among Patients with Obesity

Type 2 Diabetes Mellitus Facilitates Shift of Adipose-Derived Stem Cells Ex Vivo Differentiation toward Osteogenesis among Patients with Obesity

The therapeutic effect of adipose-derived stem cells on soft tissue injury after radiotherapy and their value for breast reconstruction

Application of telomere biology and telomerase in mesenchymal stem cells

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