Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin

Mannu, Luisa; Paba, Antonio; Daga, Elisabetta; Comunian, Roberta; Zanetti, Stefania Anna Lucia; Dupré, Ilaria; Sechi, Leonardo Antonio

doi:10.1016/s0168-1605(03)00191-0

Cited by 240 publications

(237 citation statements)

References 51 publications

Supporting

Mentioning

160

Contrasting

Unclassified

Order By: Relevance

“…Cueto-Vigil et al (2010) found sensitivity to clindamycin, erythromycin, penicillin and tetracycline by most of enterococci samples isolated from cheese, such as the enterococci isolated from sheep milk in this work. Mannu et al (2003), when comparing susceptibility to antimicrobials of enterococci from different origins, found that enterococci from raw sheep milk were sensitive to penicillin, tetracycline and vancomycin, and the ones isolated from sheep feces were sensitive to vancomycin, moderately sensitive to tetracycline and resistant to penicillin. This work leads to the supposition that the enterococci isolated from sheep milk in this study are from milk and not from feces -or any other contamination.…”

Section: Resultsmentioning

confidence: 99%

Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

Acúrcio

Souza

Nunes

et al. 2014

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

RESUMOEspécies de bactérias ácido-lácticas foram identificadas em nível molecular em leite das raças ovinas Lacaune, Santa Inês e suas mestiças, e o seu potencial probiótico in vitro foi avaliado. As espécies identificadas foram Enterococcus faecium (56,25%), E. durans (31,25%) e E. casseliflavus (12,5%). Nenhuma outra espécie de bactéria ácido-láctica, como Lactobacillus sp., foi identificada. A maioria dos enterococos isolados foi resistente ao pH gástrico (2.0) e a 0,3% de oxgall. Todos os enterococos testados foram resistentes à ceftazidima, oxacilina e estreptomicina e sensíveis à clindamicina, eritromicina e penicilina. A resistência à ciprofloxacina, gentamicina, tetraciclina e vancomicina variou entre as amostras.

show abstract

Section: Resultsmentioning

confidence: 99%

Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

Acúrcio

Souza

Nunes

et al. 2014

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

show abstract

“…The presence of genes encoding different virulence factors was tested by PCR in all enterococcal isolates recovered in this study using primers and conditions described previously (Eaton & Gasson, 2001;Mannu et al, 2003;Poeta et al, 2006). The tested genes encoding virulence factors were: gelE (gelatinase); fsr locus (regulator of the expression of gelE); cyl operon (cytolysin); cpd (pheromone determinant); hyl (hyaluronidase); agg (aggregation substance); esp (extracellular surface protein); and ace (accessory colonization factor).…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

et al. 2011

View full text Add to dashboard Cite

To study the prevalence of vancomycin-resistant enterococci (VRE) and extended-spectrum b-lactamase (ESBL)-containing Escherichia coli isolates, and the mechanisms of resistance implicated, 220 faecal samples from wild birds were collected between 2006 and 2010 in the Azores Archipelago. Samples were spread on SlanetzÁBartley agar plates supplemented with 4 mg/l vancomycin and on Levine agar plates supplemented with 2 mg/l cefotaxime for VRE and ESBL-containing E. coli isolation, respectively. vanA-containing enterococcal isolates (four Enterococcus faecium and two Enterococcus durans) and vanC-1 Enterococcus gallinarum isolates were detected in six and seven faecal samples, respectively. VRE isolates showed ampicillin (n 011), ciprofloxacin (n 09), tetracycline (n06), erythromycin (n 05), quinupristin/dalfopristin (n 03) and high-level kanamycin resistance (n 01). The tet(L) and/or tet(M) gene was found in all tetracycline-resistant isolates and the erm(B) gene in all erythromycin-resistant isolates. Three vanAcontaining E. faecium and two E. gallinarum presented specific sequences of the Tn5397 transposon. Four VRE isolates harboured the ace virulence gene. One faecal sample revealed one ESBL-containing E. coli isolate that belongs to the A phylogenetic group, showed a phenotype of resistance to b-lactams and tetracycline, and harboured the bla CTX-M-14 , bla SHV-12 and the tet(A) genes. To our knowledge, this is the first study to focus on defining the prevalence of VRE and/or ESBL-containing E. coli strains in wild birds from the Azores. The data recovered are essential to improve knowledge about the dissemination of resistant strains through wild ecosystems and their possible implications by transferring these resistances to other animals or to humans.

show abstract

“…본 연구 결과 장알균의 erythromycin과 tetracycline에 대한 내성균의 비율이 79.2%와 76.3%로 높게 나 타났으며 이러한 결과는 다른 국내, 외 다른 연구 결과와도 유사 한 경향을 보이고 있음을 알 수 있으며 (Mannu et al, 2003;Kwon et al, 2007;Jeong et al, 2008;Nam et al, 2009) 우리나 라 축산물 유래 장알균의 erythromycin과 tetracycline에 대한 내 성비율이 높음을 알 수 있었다( Table 2).…”

Section: 장알균의 항생제 감수성 유형unclassified

Distribution of Multidrug Efflux Pump Genes in Enterococci spp. Isolated from Bovine Milk Samples and Their Antibiotic Resistance Patterns

Kang¹,

Lee²,

Choi

2013

The Korean Journal of Microbiology

View full text Add to dashboard Cite

The major aim of this study was to investigate the distribution of genes that encode multidrug efflux pumps in Enterococci spp. isolates from bovine milk samples and antibiotic resistance patterns of these strains. of different species of Enterococci, eme(A) was also detected in four strains of E. faecium, seven strains of E. avium, four strains of E. durans and two strains of E. raffinosus. efr(A) was also detected in two strains of E. faecium and two strains of E. durans and efr(B) was also detected in four strains of E. faecium, five strains of E. avium and four strains of E. durans. This means the possibility of co-transfer of resistance genes between Enterococci species in natural environment. These results are the first report describing the presence of same multidrug efflux pumps in different species of Enterococci in Korea.

show abstract

Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin

Cited by 240 publications

References 51 publications

Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

Isolation, enumeration, molecular identification and probiotic potential evaluation of lactic acid bacteria isolated from sheep milk

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

Distribution of Multidrug Efflux Pump Genes in Enterococci spp. Isolated from Bovine Milk Samples and Their Antibiotic Resistance Patterns

Contact Info

Product

Resources

About