Comparison of the in vitro effect of eicosapentaenoic acid (EPA)-derived lipoxygenase metabolites on human platelet function with those of arachidonic acid

Takenaga, Mitsuko; Hirai, Aizan; Terano, Takashi; Tamura, Yasushi; Kitagawa, Haruo; Yoshida, Sho

doi:10.1016/0049-3848(86)90248-3

Cited by 48 publications

(16 citation statements)

References 21 publications

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“…While the predominant metabolite, 12-HETE, has been shown to have contradicting roles, other 12-LOX derived metabolites from EPA, DHA, DPA, and DGLA (12-HEPE, 11/ 14-hydroxydocosahexaenoic acid (11/14-HDHA), 11/14-hydroxydocosapentaenoic acid (11/14-HDPA), and 12-hydroxyeicosatrienoic acid (12-HETrE)) [70, 76, 81] have been shown to exert anti-platelet or anti-thrombotic in vivo effects. These metabolites vary in potency and ability to be synthesized.…”

Section: Regulation Of Platelet Function By 12-lox-derived Metabolitesmentioning

confidence: 99%

The expansive role of oxylipins on platelet biology

2017

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In mammals, three major oxygenases, cyclooxygenases (COXs), lipoxygenases (LOXs), and cyto-chrome P450 (CYP450), generate an assortment of unique lipid mediators (oxylipins) from polyunsaturated fatty acids (PUFAs) which exhibit pro- or anti-thrombotic activity. Over the years, novel oxylipins generated from the interplay of theoxygenase activity in various cells, such as the specialized pro-resolving mediators (SPMs), have been identified and investigated in inflammatory disease models. Although platelets have been implicated in inflammation, the role and mechanism of these SPMs produced from immune cells on platelet function are still unclear. This review highlights the burgeoning classes of oxylipins that have been found to regulate platelet function; however, their mechanism of action still remains to be elucidated.

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Section: Regulation Of Platelet Function By 12-lox-derived Metabolitesmentioning

confidence: 99%

The expansive role of oxylipins on platelet biology

2017

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“…12-hydroxyeicosatetraenoic acid (12-HETE), formed by the enzyme 12-lipoxygenase (12-LOX), is another major platelet-derived eicosanoid, although its role in platelet function remains unclear. Reports have suggested 12-HETE to have both anti-and pro-thrombotic properties [10][11][12]. It has also been suggested to play a role in promoting expression of platelet CD62 (P-selectin) [13] and is elevated in patients with essential hypertension [14].…”

Section: Introductionmentioning

confidence: 99%

12-hydroxyeicosatetraenoic acid is associated with variability in aspirin-induced platelet inhibition

Maskrey

Rushworth

Law

et al. 2014

Journal of Inflammation

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Background: Aspirin is one of the most widely used non-steroidal anti-inflammatory drugs (NSAIDs). It is also a commonly used anti-platelet drug, which inhibits the formation of the platelet activator, thromboxane A 2 (TxA 2 ) via inhibition of cyclooxygenase-1 (COX-1). However, the presence of a patient subset that fails to respond to aspirin despite reduced TxA 2 concentrations suggests that the effect of aspirin might be more complex than exclusive COX-1 inhibition. Methods: In this study we evaluated the impact of in vivo oral administration of a standard anti-platelet dose (75 mg) of aspirin in healthy volunteers on the acute impact of in vitro collagen-mediated platelet aggregation and generation of platelet-derived TxA 2 and the 12-lipoxygenase (LOX) metabolite 12-hydroxyeicosatetraenoic acid (12-HETE). The eicosanoids were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

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“…Metabolism of EPA, and other n-3 fatty acids, in the cyclooxygenase and lipoxygenase pathways, yields products with altered activities, compared to arachidonic acid metabolites [C20:4 (n = 6)] [12][13][14]. For example, the bleeding abnormalities in people consuming marine diets are chiefly due to their inability to synthesize throm boxane Aj (TxAj), a potent platelet aggrega tor; TxAi, derived from n-3 fatty acids, has a greatly attenuated ability to aggregate plate lets [2,15].…”

Section: Introductionmentioning

confidence: 99%

“…Fish oil (FO)-enriched diets are associ ated with decreased inflammatory responses [1], loss of platelet function [2,3], regression of glomerulonephritis in certain autoim mune mouse strains [4][5][6], changes in immu noglobulin synthesis [7][8][9], and altered tu mor rejection [10,11]. The mechanism(s) by which changes in dietary fat intake affect these processes is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Dietary Fat Modulation of in vitro Lymphocyte Function

Alexander

Smythe²

1988

Ann Nutr Metab

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Mitogen responsiveness of spleen cells from NZB/NZW mice fed one of three defined diets was compared. The fat source of one diet was 20% lard (L, saturated fat), the second 20% polyunsaturated (corn oil), and the third 20% polyunsaturated (fish oil, FO). No differences in stimulation indices were observed. To determine whether the lipid components in media serum supplements were altering our results, we compared splenic mitogen responses in nonautoimmune mice fed either the FO or the L diet with in vitro culture in serum-free medium, alone, or with different serum supplements. Supplements included 10% fetal calf serum and 1 and 5 % mouse sera from rodent chow-fed, L-fed or FO-fed mice. Spleen cells from BALB/c mice fed FO had consistently lower stimulation levels as compared to the other diet groups regardless of media supplementation. Capping experiments designed to directly assess membrane fluidity in lymphocytes from mice fed either the L, FO or lab chow diets revealed no differences. Although in vitro membrane turnover undoubtedly occurs during blastogenesis, we found that culturing cells in media with different serum supplements did not alter relative mitogen responses.

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Comparison of the in vitro effect of eicosapentaenoic acid (EPA)-derived lipoxygenase metabolites on human platelet function with those of arachidonic acid

Cited by 48 publications

References 21 publications

The expansive role of oxylipins on platelet biology

The expansive role of oxylipins on platelet biology

12-hydroxyeicosatetraenoic acid is associated with variability in aspirin-induced platelet inhibition

Dietary Fat Modulation of in vitro Lymphocyte Function

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