Comparison of the in vitro adhesion of K88, K99, F41 and P987 positive Escherichia coli to intestinal villi of 4- to 5-week-old pigs

Cox, Eric; Houvenaghel, A.

doi:10.1016/0378-1135(93)90003-p

Cited by 46 publications

(24 citation statements)

References 26 publications

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“…Such conditions would be optimal for the expression of both K99 and 987P, suggesting that these fimbriae may be preferentially expressed in the distal small intestine. Moreover, it has been shown that the host receptors for 987P fimbriae are located throughout the piglet intestine and not limited to any particular intestinal region (Cox and Houvenaghel, 1993), despite the fact that 987P fimbriated ETEC are found mainly to bind to distal regions in vivo (Nagy et al, 1976;Moon et al, 1979). This supports a model whereby regulation of 987P fimbrial expression is a major factor in determining the initial site of intestinal binding.…”

Section: Discussionmentioning

confidence: 86%

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Edwards

Schifferli

1997

Molecular Microbiology

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SummaryAn early process in the pathogenesis of enteric bacteria is colonization of the intestinal epithelium leading to local multiplication, pathophysiological interactions with the host and further spreading. Attachment is typically mediated by bacterial fimbriae, which are selectively expressed during growth in the intestine. Here we report an analysis of the regulation of 987P fimbrial expression of enterotoxigenic Escherichia coli (ETEC). Expression of both fasH, the transcriptional activator of the 987P fimbrial genes, and fasA, the major fimbrial subunit, is regulated in response to a variety of environmental stimuli. We have found that expression of fasH is regulated in response to the carbon status of the growth medium by the cAMP-CRP complex. Moreover, fasH is regulated in response to both the nitrogen status of the growth medium and the external pH. Expression of fasA is activated by FasH, and is also selectively regulated in response to growth temperature by HNS. Regulation of fimbrial expression by carbon and/or nitrogen gradients is proposed to provide a mechanism that allows preferential colonization of different segments of the intestine by various enteropathogens, such as ETEC, enteropathogenic E. coli and Vibrio cholerae.

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Section: Discussionmentioning

confidence: 86%

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Edwards

Schifferli

1997

Molecular Microbiology

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“…For a cut-off value of 95 for instance, the estimated sensitivity and specificity for the adhesion test F4ac equal, is respectively 23% and 84%. Based on the experiments of Cox and Houvenaghel (1993), a cut-off value of 5 bacteria was chosen. With this cutoff value the estimated sensitivity and specificity for the adhesion test for both F4ab and F4ac equal respectively 100% and 24%.…”

Section: Resultsmentioning

confidence: 99%

“…The adhesion of bacteria was evaluated by counting the number of bacteria adhering along a 50 mm villous brush border length at 20 randomly selected places with a phase-contrast microscope at a magnification of 600. Adhesion of more than 5 bacteria per 250 mm villous brush border length was noted as positive (Cox and Houvenaghel, 1993). Each villous adhesion test included some negative controls.…”

Section: The In Vitro Villous Adhesion Assay For F4r Characterizationmentioning

confidence: 99%

Screening of pigs resistant to F4 enterotoxigenic Escherichia coli (ETEC) infection

Rasschaert

Verdonck

Goddeeris

et al. 2007

Veterinary Microbiology

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The present study analysed quantitatively the mucin 4 polymorphism for determing the F4ac/ab receptor status of a total of 63 pigs by comparing it with the in vitro villous adhesion assay. The probability of a susceptible genotype for the mucin 4 increases significantly with increasing F4ab or F4ac ETEC adhesion per 250 mm villi (P = 0.029 for F4ab, P = 0.030 for F4ac), with the odds ratio for each unit increase of F4ab or F4ac equal to, respectively, 1.036 (95% CI [1.004-1.069]) and 1.018 (95% CI [1.002-1.034]). In the phenotypic in vitro villous adhesion test, a cut-off value of 5 bacteria was chosen as a criteria for the distinction between an F4R positive and F4R negative pig. The sensitivity and specificity for the in vitro villous adhesion test, with the genotyping test for mucin 4 as golden standard, is 100% and 24%, respectively, for F4ab as well as F4ac. Absence of adhesion of F4ac and F4ab ETEC to the villous brush borders was not associated with genotypic resistance suggesting that there is at least one other receptor for F4ab/ac Escherichia coli. As a consequence, not only mucin 4 gene polymorphism but also expression of these other receptor(s) has to be included in a screening assay for F4ac/ab receptor negative pigs. #

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“…In order to determine the presence of the F18R on small intestinal villous enterocytes, an in vitro villous adhesion assay was performed as described previously (Cox and Houvenaghel, 1993;Verdonck et al, 2002). After euthanasia of the pigs, a 20 cm intestinal segment was excised from the mid jejunum of the pig and rinsed three times with icecold PBS.…”

Section: In Vitro Villous Adhesion Assaymentioning

confidence: 99%

The age-dependent expression of the F18+ E. coli receptor on porcine gut epithelial cells is positively correlated with the presence of histo-blood group antigens

Coddens

Verdonck

Tiels

et al. 2007

Veterinary Microbiology

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F18+ Escherichia coli have the ability to colonize the gut and cause oedema disease or post-weaning diarrhoea by adhering to specific F18 receptors (F18R) on the porcine epithelium. Although it is well established that a DNA polymorphism on base pair 307 of the FUT1 gene, encoding an a(1,2)fucosyltransferase, accounts for the F18R phenotype, the F18R nature is not elucidated yet. The aim of the present study was to investigate the correlation between the presence of H-2 histo-blood group antigens (HBGAs) or its derivative A-2 HBGAs on the porcine gut epithelium and F18 + E. coli adherence. A significant positive correlation was found between expression of both the H-2 (r = 0.586, P < 0.01) and A-2 (r = 0.775, P < 0.01) HBGAs and F18 + E. coli adherence after examination of 74 pigs aged from 0 to 23 weeks. The majority of the genetically resistant pigs (FUT1M307 A/A ) showed no HBGA expression (91.7%) and no F18 + E. coli adherence (83.3%). In addition, it was found that F18R expression levels rise with increasing age during the first 3 weeks after birth and that F18R expression is maintained in older pigs (3-23 weeks old). Taken together, these data suggest that, apart from H-2 HBGAs, A-2 HBGAs might be involved in F18 + E. coli adherence.

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Comparison of the in vitro adhesion of K88, K99, F41 and P987 positive Escherichia coli to intestinal villi of 4- to 5-week-old pigs

Cited by 46 publications

References 26 publications

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Differential regulation of fasA and fasH expression of Escherichia coli 987P fimbriae by environmental cues

Screening of pigs resistant to F4 enterotoxigenic Escherichia coli (ETEC) infection

The age-dependent expression of the F18+ E. coli receptor on porcine gut epithelial cells is positively correlated with the presence of histo-blood group antigens

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