Comparison of the Impact of Different Administration Routes on the Efficacy of a Thermoresistant Newcastle Disease Vaccine in Chickens

Hassanzadeh, Mohammad; Abdoshah, Mohammad; Yousefi, Ali Reza; Masoudi, Shahin

doi:10.1089/vim.2019.0125

Cited by 5 publications

(8 citation statements)

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“…In terms of mortality, two early deaths on the third day pi were recorded in birds of the mixed intraocular–intranasal inoculated group [ 52 , 53 ]. A similar early higher virus systemic distribution via the ocular route was reported in a recent study [ 54 ]. Although deaths began later on the fifth day pi in the other groups, these findings are in line with some previous studies [ 18 , 55 ].…”

Section: Discussionsupporting

confidence: 87%

“…The lesions, as well as their incidence, nature, and affected organs, in relation to the infection route might yield insights for a better approach to vaccination. A previous study revealed that the immunization of chicks, through eye drop, induced the highest hemagglutination inhibition antibody titers at 20 days of age, followed by immunization through distilled water and through feed [ 54 ]. These findings revealed the efficacy of immunization through eye.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

EL-Morshidy¹,

Abdo

Elmahallawy

et al. 2021

Animals

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Newcastle disease virus (NDV) remains a constant threat to the poultry industry. There is scarce information concerning the pathogenicity and genetic characteristics of the circulating velogenic Newcastle disease virus (NDV) in Egypt. In the present work, NDV was screened from tracheal swabs collected from several broiler chicken farms (N = 12) in Dakahlia Governorate, Egypt. Real-time reverse transcriptase polymerase chain reaction (RRT-PCR) was used for screening of velogenic and mesogenic NDV strains through targeting F gene fragment amplification, followed by sequencing of the resulting PCR products. The identified strain, namely, NDV-CH-EGYPT-F42-DAKAHLIA-2019, was isolated and titrated in the allantoic cavity of 10 day old specific pathogen-free (SPF) embryonated chicken eggs (ECEs), and then their virulence was determined by mean death time (MDT) and intracerebral pathogenicity index (ICPI). The pathogenicity of the identified velogenic NDV strain was also assessed in 28 day old chickens using different inoculation routes as follows: intraocular, choanal slit, intranasal routes, and a combination of both intranasal and intraocular routes. In addition, sera were collected 5 and 10 days post inoculation (pi) for the detection of NDV antibodies by hemagglutination inhibition test (HI), and tissue samples from different organs were collected for histopathological and immunohistochemical examination. A series of different clinical signs and postmortem lesions were recorded with the various routes. Interestingly, histopathology and immunohistochemistry for NDV nucleoprotein displayed widespread systemic distribution. The intensity of viral nucleoprotein immunolabeling was detected within different cells including the epithelial and endothelium lining, as well as macrophages. The onset, distribution, and severity of the observed lesions were remarkably different between various inoculation routes. Collectively, a time-course comparative pathogenesis study of NDV infection demonstrated the role of different routes in the pathogenicity of NDV. The intranasal challenge was associated with a prominent increase in NDV lesions, whereas the choanal slit route was the route least accompanied by severe NDV pathological findings. Clearly, the present findings might be helpful for implementation of proper vaccination strategies against NDV.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

EL-Morshidy¹,

Abdo

Elmahallawy

et al. 2021

Animals

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“…The appropriate serological immune response in chickens received ND.TR.IR was supported by the result of the challenge efficacy test, where the vaccinated chickens were fully protected against vNDV infection. It has been documented that chickens with HI antibody titers ≥2 3 would be protected following the challenge efficacy test (W. Allan & Gough, 1974; Hossain et al., 2010; Hassanzadeh et al., 2020; Van Boven et al., 2008). However, there are pieces of evidence showing that the chickens with lower titer also may survive after the challenge (Meers & Spradbrow, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that the efficacy of heatresistant ND vaccines is kept up to 2 months at 9-29 • C, and 2 weeks at 30-37 • C (Alders, 2014). In this regard, previous investigations (Fentie et al, 2014;Hassanzadeh et al, 2020;Nasser et al, 2000;Van Boven et al, 2008) also showed a high protection against vNDV in chickens vaccinated by I-2.…”

Section: Introductionmentioning

confidence: 91%

Thermoresistant Newcastle disease vaccine effectively protects SPF, native, and commercial chickens in challenge with virulent virus

Abdoshah

Hassanzadeh

Masoudi

et al. 2022

Veterinary Medicine & Sci

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Background Due to the more stability and a better homogenecity in immune response, the use of thermoresistant vaccines in different chicken types has been increased. Objective This study aimed to evaluate the efficacy of a newly developed Newcastle disease vaccine (ND.TR.IR) originating from I‐2 strain in specific pathogen‐free (SPF) and native and broiler chickens. Methods Following determination of pathogenicity indices on the candidate seed, three efficacy examinations were conducted. In the first experiment, 120 1‐day‐old SPF chickens were randomly allocated to six groups and either vaccinated with ND.TR.IR via eye drop at 1, 7, and 21 days of age (V1, V7, and V21), or considered as non‐vaccinated control groups (C1, C7, and C21). At 20th post‐vaccination day, sera hemagglutination inhibition (HI) antibody titres against ND virus (NDV) were measured and then the chickens were challenged by virulent NDV (vNDV). In the second and third experiments, the efficacy of ND.TR.IR vaccine was compared to routine vaccination program (B1 and LaSota) in native and broiler chickens that were vaccinated at 10 and 20 days of age, respectively. The HI antibody titres were measured on 10, 20, 30, and 40 days of age, and also challenge efficacy test with vNDV was conducted on 30 days of age. Results The studied virus, as a vaccinal seed, complied with the pathogenicity indices of avirulent NDV and molecular identity of I‐2 strain. In the efficacy evaluation trials, the vaccinated chickens had higher HI antibody titres against NDV compared with their corresponding control chickens (p < 0.05). Results of the challenge tests indicated 95% and 100% protection against vNDV in native, SPF, and broiler‐vaccinated chickens, respectively. Conclusions The present findings indicated that administration of ND.TR.IR induced appropriate HI antibody titres against NDV in SPF, native, and broiler chickens associated with good protection in efficacy test.

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“…В данный момент на птицеводческих предприятиях, согласно плану профилактических, противоэпизоотических мероприятий, применяют до десяти различных методов вакцинации птицы: внутримышечный, интраназальный, мелкодисперсный (аэрозольный) и крупнодисперсным распылением (спрей), накожный, подкожный, пункция в перепонку крыла, пероральный, интраокулярный, клоачный [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

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Efficiency of Various Poultry Vaccination Methods in Industrial Poultry Farming

Zelenkova,

Ustyantsev,

Pakhomova

et al. 2023

Vetpat

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Introduction. Due to the growth of poultry stocking density in the allotted area in conditions of the industrial poultry farming, the issue of ensuring the epizootic welfare through vaccine-prophylaxis proves to be relevant. Another important aspect of poultry farming is reducing production costs, which could be achieved, among other things, through implementation of the cost-efficient methods of vaccination. A manual mode of spray vaccination method, which is currently used at the poultry farms, has a disadvantage because the dose of vaccine received by different birds may be unequal. However, under modern conditions, in order to increase the labour productivity and efficiency, the efforts are made to get the poultry vaccination process automated. The vaccination frame spray machines used in the industrial poultry farming have not been studied or evaluated before in a comparative aspect. Thus, the purpose of this research is to compare two methods of spray vaccination in chickens at the operating poultry farm with regard to the biological efficiency and economic profitability.Materials and Methods. The objects of the study were the young Brown Nick crossbreed chickens (n=46 heads). Vaccination was carried out in the young poultry at the age of 42-days. During the research, the dried live Newcastle disease and avian infectious bronchitis vaccines were used. To control the quality of vaccination, the amount of antibodies was estimated in the blood serum of poultry aged 56, 70 and 95 days old by ELISA method with TICAN equipment. Two groups of poultry were studied. In group 1 the “DESVAK” hand sprayer was used (“DESVAK” KIT– 2 model, produced by Ceva Santé Animale), in group 2 the automated frame spray machine was used (“SIMBIO ITERIS” model, produced by SIMBIO).Results. As a result of studies, in group 2 the higher intensity of immunity was obtained, showing on average the following figures: 56 days – antibody titre was 29 % higher (p≤0.05); 70 days – antibody titre was 18 % higher (p≤0.005); 95 days – antibody titre was 15 % higher (p≤0.05) compared to group 1. Economic efficiency was calculated based on the indicators of labour costs and labour intensity of the vaccination process: when using a frame spray machine, the costs turned out to be 2.3 times lower (1,131.4 person-rubbles) than with the DESVAK sprayer (2,590.4 person-rubbles). Additionally, the labour intensity of the vaccination process per head decreased by 5 times. In group 1 it was 0.0015 person-hour/head, in group 2 – 0.0003 person-hour/head.Discussion and Conclusion. Based on the results of the study, it was found that the spray vaccination method by means of the frame spray machine (the automated mode) is more efficient according to both biological and economic indicators compared to the manual mode. Taking into account all of the above, the frame spray machines could be recommended to the veterinary specialists for carrying out vaccine prophylaxis at the poultry farms by the spray method.

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Comparison of the Impact of Different Administration Routes on the Efficacy of a Thermoresistant Newcastle Disease Vaccine in Chickens

Cited by 5 publications

References 23 publications

Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

Pathogenesis of Velogenic Genotype VII.1.1 Newcastle Disease Virus Isolated from Chicken in Egypt via Different Inoculation Routes: Molecular, Histopathological, and Immunohistochemical Study

Thermoresistant Newcastle disease vaccine effectively protects SPF, native, and commercial chickens in challenge with virulent virus

Efficiency of Various Poultry Vaccination Methods in Industrial Poultry Farming

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