Comparison of the
            <i>Bordetella pertussis</i>
            and
            <i>Bordetella parapertussis</i>
            Isolates Circulating in Saint Petersburg between 1998 and 2000 with Russian Vaccine Strains

Kourova, Natacha; Caro, Valérie; Weber, Christian; Thiberge, Sabine; Чупринина, Р П; Tseneva, Galina; Guiso, Nicole

doi:10.1128/jcm.41.8.3706-3711.2003

Cited by 29 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it is important to note that pursuing culture is essential in order to analyze the spatio-temporal evolution of Bordetella pertussis according to the immunity of the human population. We observed that the collected isolates were very similar to each other (all PFGE group IV), confirming our previous results obtained in Argentina (4), Europe (7), Japan (14), Russia (17), and France (6,26). We demonstrated that the PFGE group IV subgroup gamma, first observed in Finland and more recently in France (5, 7), is now also circulating in the United States.…”

Section: Discussionsupporting

confidence: 80%

Comparison of Serological and Real-Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007

et al. 2008

Self Cite

View full text Add to dashboard Cite

Bacterial culture for diagnosing pertussis infection has high specificity but poor sensitivity and is slow. Highly sensitive real-time PCR assays and single-serum pertussis serology have been developed to overcome these limitations, but there are few data available on the relative sensitivities and specificities of such assays for pertussis diagnosis. Using data on 195 participants (>7 years old) from an epidemiological study, we assessed the sensitivity, specificity, and performance (Youden index) for pertussis diagnosis of the pertussis toxin enzyme-linked immunosorbent assay (using single and paired serology) and of real-time PCR assays (using the IS481 and ptxA-Pr targets). All available diagnostic information (clinical and laboratory) was pooled to serve as the gold standard. Single serology was the most efficient diagnostic test (Youden index, 0.57 to 0.58), with relatively high sensitivity (>64%) and high specificity (>90%), independent of the cutoff level. IS481 PCR performance was superior to that of ptxA-Pr PCR, and it was the second-most-efficient tool (Youden index, 0.30). Performing both ptxA-Pr and IS481 PCRs did not improve diagnostic performance. The greatest test efficiency (Youden index, 0.69 to 0.74) was achieved when single-serum serology was used in combination with IS481 or ptxA-Pr PCR or paired serology. Combining single serology with one PCR or paired serology increased the sensitivity with an associated limited decrease in specificity. The most specific tests for diagnosis of pertussis were single serology and ptxA-Pr PCR, and the most sensitive diagnostic tool was the combination of IS481 PCR with single serology.Currently there is no satisfactory gold standard technique for laboratory confirmation of a pertussis infection. Although culture is highly specific, sensitivity is low and declines with the duration of illness, and the method may take up to 7 days to provide a result. Using culture alone to diagnose pertussis is likely to lead to underreporting of pertussis cases (8). Serological assays, using one or two serum samples, have been developed to improve the sensitivity of the pertussis diagnosis, but they have a lower specificity than culture (3,9,20,25). PCR techniques were developed to overcome these limitations, but despite a consensus meeting in 2005 (24), these techniques still require further standardization and optimization. To date there are few reports (8) in which the sensitivities and specificities of these different techniques are compared using a population exposed to pertussis.The objective of this study was to compare the sensitivities and specificities of the current most widely used techniques to diagnose pertussis in exposed populations, namely, two PCR methods (IS481 and ptxA-Pr targets) and single and paired serology for detection of antipertussis toxin (PT) antibodies in the serum of the patients using enzyme-linked immunosorbent assay (ELISA) with two different cutoffs (two-or fourfold change for paired serology and Ն100 or Ն125 ELISA units [EU]/ml for single...

show abstract

Section: Discussionsupporting

confidence: 80%

Comparison of Serological and Real-Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…Researchers point to many reasons for the increased incidence of pertussis, including improved surveillance, waning immunity, and pathogen adaptation. The last possibility is supported by the fact that divergence in the protective antigens pertussis toxin (Ptx) and pertactin (Prn) has been observed between vaccine strains and the strains circulating in many countries (2,7,9,12,13,17,18,20,24,(31)(32)(33). In general, it was found that "non-vaccine-type" alleles of ptxA and prn (i.e., ptxA1, prn2, and prn3) have gradually replaced the "vaccinetype" alleles (i.e., ptxA2, ptxA4, and prn1) that were in circulation earlier.…”

mentioning

confidence: 94%

“…In general, it was found that "non-vaccine-type" alleles of ptxA and prn (i.e., ptxA1, prn2, and prn3) have gradually replaced the "vaccinetype" alleles (i.e., ptxA2, ptxA4, and prn1) that were in circulation earlier. Analysis of strains isolated in Russia (Moscow and St. Petersburg) from 1998 to 2002 also revealed that strains with the ptxA1 and the prn2 or prn3 alleles prevailed in circulation (12,14). Possible variations in nucleotide sequences in 15 other genes encoding surface-exposed proteins have also been studied.…”

mentioning

confidence: 99%

Antigenic Divergence between Bordetella pertussis Clinical Isolates from Moscow, Russia, and Vaccine Strains

Borisova

Kombarova

Zakharova

et al. 2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

We analyzed temporal changes in the frequencies of the ptxA, prn, fim2, and fim3 alleles in Bordetella pertussis strains isolated from pertussis patients in Moscow, Russia, from 1948 to 2004. The three strains used for the whole-cell vaccine harbored the alleles ptxA2, ptxA4, prn1, fim2-1, and fim3A. Vaccine-type alleles of ptxA (ptxA2 and ptxA4) were characteristic for all prevaccination strains and for 96% of the strains isolated in the 1960s and 1970s. At the beginning of the 1970s, ptxA2 and ptxA4 were replaced by the ptxA1 allele. In the 1980s and to the present, strains with ptxA1 were predominant in the B. pertussis population. All prevaccination strains harbored the prn1 allele, which corresponds to the vaccine-type allele. In subsequent years, the proportion of strains with the prn1 allele decreased and the proportion of prn3 and prn2 strains increased. From 2002 to 2004 strains with prn2 or prn3 were predominant in the B. pertussis population. The vaccine-type alleles fim2-1 and fim3A were found in all prevaccination strains and in 92% of the strains isolated from 1960 to 1989. The fim2-2 and fim3B alleles were first observed at the beginning of the 1980s. In subsequent years, these strains became predominant. Together with waning immunity, the antigenic divergence between vaccine strains and clinical isolates observed in the Moscow area may explain the persistence of pertussis, despite the high rates of vaccine coverage. The results demonstrate that the selection of B. pertussis strains for vaccine manufacturing must be based on a thorough study of the B. pertussis population.

show abstract

“…Since Mooi et al (20) found that the circulating clinical strains had antigens distinct from those of vaccine strains, they proposed that the circulating clinical strains might have escaped the immunity provided by pertussis vaccination. The antigenic divergence between recently circulating strains and vaccine strains has been reported in European and North American countries (3,6,9,16,19,22,(29)(30)(31) but not in Asian countries.…”

mentioning

confidence: 99%

Antigenic Divergence Suggested by Correlation between Antigenic Variation and Pulsed-Field Gel Electrophoresis Profiles of Bordetella pertussis Isolates in Japan

et al. 2004

View full text Add to dashboard Cite

Antigenic divergence has been found between Bordetella pertussis vaccine strains and circulating strains in several countries. In the present study, we analyzed B. pertussis isolates collected in Japan from 1988 to 2001 using pulsed-field gel electrophoresis (PFGE) and sequencing of two virulence-associated proteins. The 107 isolates were classified into three major groups by PFGE analysis; 87 (81%) were type A, 19 (18%) were type B, and 1 (1%) was type C. Sequence analysis of the S1 subunit of pertussis toxin (ptxS1) and adhesion pertactin (prn) genes revealed the presence of two (ptxS1A and ptxS1B) and three (prn1, prn2, and prn3) variants, respectively, in the isolates. Among those isolates, 82 (95%) of the 87 type A strains and the type C strain had the same combination of ptxS1B and prn1 alleles (ptxS1B/prn1) as the Japanese vaccine strain. On the other hand, 17 (90%) of 19 type B strains had an allele (ptxS1A/prn2) distinct from that of the vaccine strain. A correlation was found between the antigenic variation and the PFGE profile in the isolates. In addition, the frequency of the type B strain was 0, 27, 0, 42, and 37% of the isolates in the periods

show abstract

Comparison of the Bordetella pertussis and Bordetella parapertussis Isolates Circulating in Saint Petersburg between 1998 and 2000 with Russian Vaccine Strains

Cited by 29 publications

References 22 publications

Comparison of Serological and Real-Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007

Comparison of Serological and Real-Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007

Antigenic Divergence between Bordetella pertussis Clinical Isolates from Moscow, Russia, and Vaccine Strains

Antigenic Divergence Suggested by Correlation between Antigenic Variation and Pulsed-Field Gel Electrophoresis Profiles of Bordetella pertussis Isolates in Japan

Contact Info

Product

Resources

About