Comparison of the Histopathology of Immediate and Late Asthmatic and Cutaneous Responses in a Rabbit Model

Behrens, B. L.; Clark, Richard A.F.; Feldsien, Diane C.; Presley, D.; Glezen, Laurie S.; Graves, J. P.; Larsen, Gary L.

doi:10.1378/chest.87.5_supplement.153s

Cited by 15 publications

(4 citation statements)

References 3 publications

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“…periorbital swelling. The early phase of periorbital swell ing peaked at 0.5 h, the secondary phase at 6-7 h. Therefore, we conclude that ocular tis sues (like the skin [1][2][3], lung [4] and nose [8,9]) undergo a significant clinical LPR several hours after the EPR.…”

Section: Discussionmentioning

confidence: 89%

“…The subsequent inflammatory response is termed the latephase reaction (LPR). Histologic changes ac companying LPR are characterized in hu mans [1][2][3] and animals [4][5][6] by accumula-tions of inflammatory cells including eosino phils, neutrophils and lymphocytes. Human LPR have been observed in the skin [1,2], lung [7] and nose [8,9], The occurrence of the LPR in human ocular tissues has not been firmly established.…”

Section: Introductionmentioning

confidence: 99%

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Clinical and Cytologic Aspects of Ocular Late-Phase Reaction in the Guinea Pig

1992

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Conjunctival tissue of 12 guinea pigs (6 experimental animals, 6 controls) was sensitized with IgG₁ (PCA titer 1:2,000) anti sera to dinitrophenol carrier and challenged topically with hapten. Periorbital swelling, conjunctival edema and conjunctival redness were evaluated clinically at 0.5 h and hourly for 12 h, and again at 24 and 48 h. Tears were collected at each time point for cytology. Results showed an early-phase reaction (EPR) peaking at 0.5 h and a late-phase reaction (LPR) peaking at 5–7 h. Periorbital swelling was minimal 4 h after appearance of the EPR. Conjunctival edema and redness increased in both the early and late phases, but without two distinct peaks. However, individual animals in both groups did show biphasic reactions. Tear cytology showed an abnormal increase in neutrophils and eosinophils in the later time periods and was a significant way to detect ocular anaphylaxis. In conclusion, if the presence of two significant peaks of clinical inflammation after one antigen challenge is taken as the narrowest definition of a late phase in anaphylaxis, our results show an ocular LPR occurring in our animal model.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Clinical and Cytologic Aspects of Ocular Late-Phase Reaction in the Guinea Pig

1992

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“…To investigate the role of inflammation in the rabbit model of the late asthmatic response, Behrens and coworkers 41 serially reviewed pulmonary and cutaneous histopathologic changes over a 7day period after coincident intradermal injection and bronchial challenge with Alternaria antigen. In their experimental design, actively immunized animals were compared with controls at 30 minutes, 6, 24, and 48 hours, and 7 days after antigen exposure to study the progression of histologic changes in the skin and airway (Fig.…”

Section: Rabbit Model Of the Late Asthmatic Responsementioning

confidence: 99%

Inflammation and Asthma

Wilson¹,

Irvin²,

Larsen³

1987

Semin Respir Crit Care Med

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“…10,11 Animal studies using ozone exposure in dogs or antigen challenge in rabbits have documented increased numbers of neutrophils in the airways and have linked this inflammatory response to subsequent increases in airways reactivity to histamine. 12,13 When airways inflammation is blocked by neutrophil depletion, increased airways reactivity does not occur. 6,14 These data support the central role that the neutrophil may play in the production of airways inflammation, subsequent hyperreactivity, and clinical asthma.…”

Section: Cells Involved In Mediator Productionmentioning

confidence: 99%