Comparison of the hamster sperm motility assay to the mouse one-cell and two-cell embryo bioassays as quality control tests for in vitro fertilization

Gorrill, Marsha J.; Rinehart, John S.; Tamhane, Ajit C.; Gerrity, Marybeth

doi:10.1016/s0015-0282(16)54128-4

Cited by 22 publications

(9 citation statements)

References 22 publications

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“…The oocyte comet assay presented in this study was based on a novel modification of the original comet assay for white blood cells (16)(17)(18)(19)(20). The results showed that the oocyte comet assay could be used to quality control test materials used in assisted reproductive technologies, except that it was considerably faster to perform when compared with the mouse one-cell embryo bioassay (1)(2)(3)(4)(5)(6)(7)(8). The sensitivity in detecting toxicity appeared to be greater in the oocyte comet assay as suggested by the failing grade for two materials that had passed the mouse bioassay.…”

Section: Discussionmentioning

confidence: 97%

“…Testing of water, culture media, protein supplements, and contact materials at the centers of assisted reproductive technologies are presently performed using either the one-or two-cell mouse embryo bioassay (1)(2)(3)(4)(5)(6)(7)(8), the hamster epididymal sperm motility bioassay (9,10), the human sperm survival bioassay (11,12) or the hybridoma cell culture assay (13). Thus, the centers routinely perform bioassays and have become proficient in cytogenotoxic testing.…”

Section: Introductionmentioning

confidence: 99%

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Untitled

Chan

Calinisan

Corselli

et al. 2001

Journal of Assisted Reproduction and Genetics

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Untitled

Chan

Calinisan

Corselli

et al. 2001

Journal of Assisted Reproduction and Genetics

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“…Gorrill and colleagues also compared the hamster SVB to the one-cell and two-cell MEB. They reported that the hamster SVB was a more sensitive test, could be performed within 6 hours compared to 3 to 4 days required for the MEB and was less expensive to perform (Gorrill et al, 1991).…”

Section: Toxicity Testingmentioning

confidence: 99%

Quality Management Issues in the Assisted Reproduction Laboratory

Boone

Higdon

Johnson

2010

Journal of Reproductive and Stem Cell Biotechnology

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In the United States, the Clinical Laboratory Improvement Act (CLIA) of 1988 describes requirements and guidelines for implementing a quality control/quality assurance (QC/QA) program for moderate and high complexity laboratories. These requirements and guidelines apply to Assisted Reproductive Technology (ART) laboratories as well. The general topic of QC and QA as it pertains to in vitro fertilization (IVF) and embryo transfer (ET) is extensively reviewed. This review summarizes many of the QC and QA events that contribute to the advancement of knowledge in this biotechnological field. These events include control of the culture environment inside and outside of the incubator, as well as factors that affect culture media. This review also discusses, in considerable detail, the QC and the QA that pertain to equipment used within the laboratory and how to control for potential contaminants, which reside within the laboratory. This review provides evidence to indicate the need for laboratory personnel to monitor quality improvement issues on a continuous basis. Personnel must be willing to change as improvements in technology occur in order to meet the ever-evolving demands of a more difficult patient population. Suggestions for meeting these demands are offered.

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“…Die bessere Verfügbarkeit von menschlichen Samenzellen und der geringere technische Aufwand bei der Durchführung des Testverfahrens wurden als Vorteile dieser Methode genannt. Im direkten Vergleich mit der Kultivierung von Einzellembryonen oder 2-Zell-Embryonen der Maus erwies sich die Motilitätsrate von aufbereiteten Samenzellen des Hamsters oder des Menschen als signifikant sensitiver (Gorrill et al 1991).…”

Section: Beobachtung Der Motilität Von Aufbereiteten Samenzellenunclassified