Comparison of the functional features of the pump organs of Anopheles sinensis and Aedes togoi

Ha, Young-Ran; Lee, Seung−Chul; Seo, Sang Woo; Ryu, Jeongeun; Lee, Dong‐Kyu; Lee, Sang-Joon

doi:10.1038/srep15148

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…In the head, the aorta associates with the dorsal plate of the pharyngeal pump ( Fig. 2A-C), which is one of two pumps that facilitate feeding (Kim et al, 2011;Ha et al, 2015). Upon entering the prothorax, the aorta bends dorsally and then straightens such that it travels near the lateral midline with a slight, ventral slant as it crosses the mesothorax and metathorax ( Fig.…”

Section: The Location Of the Aortamentioning

confidence: 99%

Structural and functional characterization of the contractile aorta and associated hemocytes of the mosquito Anopheles gambiae

Sigle

Hillyer

2018

Journal of Experimental Biology

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The primary pump of the circulatory system of insects is a dorsal vessel that traverses the length of the insect. The anterior portion, located in the head, neck and thorax, is the aorta, and the posterior portion, located in the abdomen, is the heart. Here, we characterize the structure and function of the aorta and conical chamber of the mosquito, The aorta begins in the head with an excurrent opening located above the dorsal pharyngeal plate and ends at the thoraco-abdominal junction where it joins the conical chamber of the heart. The aorta lacks ostia, and based on the diameter of the vessel as well as the density and helical orientation of muscle, consists of three regions: the anterior aorta, the bulbous chamber, and the posterior aorta. The aorta contracts in the anterograde direction, but these contractions are independent of heart contractions and do not play a major role in hemolymph propulsion. Intravital imaging of the venous channels, the first abdominal segment and the neck revealed that hemolymph only travels through the aorta in the anterograde direction, and does so only during periods of anterograde heart flow. Furthermore, hemolymph only enters the thoraco-abdominal ostia of the conical chamber when the heart contracts in the retrograde direction, propelling this hemolymph to the posterior of the body. Finally, very few hemocytes associate with the aorta, and unlike what is seen in the periostial regions of the heart, infection does not induce the aggregation of hemocytes on the aorta.

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Section: The Location Of the Aortamentioning

confidence: 99%

Structural and functional characterization of the contractile aorta and associated hemocytes of the mosquito Anopheles gambiae

Sigle

Hillyer

2018

Journal of Experimental Biology

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“…A valve-like organ is located between both of these pumps and prevents backflow in the conduit connecting both structures. Relevant to blood feeding behaviours, the recent implementation of synchrotron X-ray microscopic computed tomography in malaria research, allowed for a detailed description of the feeding pump system located inside the head of Anopheles and Aedes mosquitoes (Ha et al, 2015). The study concluded that morphological and functional features of the pump system strongly influence the feeding behaviour of female mosquitoes, therefore influencing the transmission success of various pathogens to humans.…”

Section: Opt Enables Visualization Of the Entire Anatomy Of Intact Admentioning

confidence: 99%

3D imaging of undissected optically clearedAnopheles stephensimosquitoes infected withPlasmodiumparasites

Niz

Kehrer

Brancucci

et al. 2019

Preprint

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Malaria is a life-threatening disease, caused by Apicomplexan parasites of the Plasmodium genus. The Anopheles mosquito is necessary for the sexual replication of these parasites and for their transmission to vertebrate hosts, including humans. Imaging of the parasite within its insect vector has been attempted using multiple microscopy methods, yet most have been hampered by the presence of the mosquito's opaque cuticle, which scatters light. So far, imaging of the Plasmodium mosquito stages has depended on either sectioning or surgical dissection of important anatomical sites, such as the midgut and the salivary glands. Optical projection tomography (OPT) and light sheet fluorescence microscopy (LSFM) enable imaging fields of view in the centimeter scale whilst providing micrometer resolution. In this paper, we present reconstructions of the whole body of Plasmodium-infected, optically cleared Anopheles stephensi mosquitoes and midguts. The 3D-reconstructions from OPT imaging show detailed features of the mosquito anatomy relevant for transmission, and enable overall localization of the parasite within the vector throughout the entire mosquito life cycle. Additionally, LSFM imaging of mosquito midguts shows detailed distribution of oocysts in the midgut of intact mosquitoes.

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“…To date, many techniques such as micro-computed tomography [7], microscopic and histological analysis [8,9], and X-ray imaging [10] have been implemented for mosquito studies. However, these techniques require time-consuming sectioning processes, a complex system design, or a long imaging acquisition time.…”

Section: Introductionmentioning

confidence: 99%

OCT for non-destructive examination of the internal biological structures of mosquito specimen

Ravichandran¹,

Jeon²,

Lee³

et al. 2018

EECSI

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The Study of mosquitoes and their behavioral analysis are of crucial importance to control the alarmingly increasing mosquito-borne diseases. Conventional imaging techniques use either dissection, exogenous contrast agents. Non-destructive imaging techniques, like x-ray and microcomputed tomography uses ionizing radiations. Hence, a non-destructive and real-time imaging technique which can obtain high resolution images to study the anatomical features of mosquito specimen can greatly aid researchers for mosquito studies. In this study, the three-dimensional imaging capabilities of optical coherence tomography (OCT) for structural analysis of Anopheles sinensis mosquitoes has been demonstrated. The anatomical features of An. sinensis head, thorax, and abdomen regions along with internal morphological structures like foregut, midgut, and hindgut were studied using OCT imaging. Two-dimensional (2D) and three-dimensional (3D) OCT images along with histology images were helpful for the anatomical analysis of the mosquito specimens. From the concurred results and by exhibiting this as an initial study, the applicability of OCT in future entomological researches related to mosquitoes and changes in its anatomical structure is demonstrated.

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Comparison of the functional features of the pump organs of Anopheles sinensis and Aedes togoi

Cited by 7 publications

References 16 publications

Structural and functional characterization of the contractile aorta and associated hemocytes of the mosquito Anopheles gambiae

Structural and functional characterization of the contractile aorta and associated hemocytes of the mosquito Anopheles gambiae

3D imaging of undissected optically clearedAnopheles stephensimosquitoes infected withPlasmodiumparasites

OCT for non-destructive examination of the internal biological structures of mosquito specimen

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