Comparison of the Fat Elimination Between Long-Chain Triglycerides and Medium-Chain Triglycerides in Rats With Ischemic Acute Renal Failure

Abstract

To guide the administration of fat emulsion in the nutritional support of acute renal failure (ARF), pharmacokinetic analysis with an one-compartment open model after bolus intravenous injection was performed to compare the elimination kinetics of long-chain triglycerides (LCT) and medium-chain triglycerides (MCT) in ischemic acute renal failure rats. Sprague-Dawley rats were randomized into four groups, namely LCT normal group, LCT ARF group, MCT normal group and MCT ARF group. The model of ischemic acute renal failure was induced by clamping the left renal artery for 60 min and contralateral nephrectomy. All the rats were fasted with water ad libitum for 10 h before 0.3 g/kg body weight of 10% Intralipid (LCT) or 10% Lipofundin (MCT: LCT = 50:50) was injected as a bolus to them via the tail vein. The serum triglyceride concentration was determined at 2, 10, 40, 70, 100, 130 and 160 min after intravenous injection for kinetic analysis. The results showed that the elimination rate constant (ke) of LCT ARF group was significantly decreased, while the half life period (t1/2) of it was significantly longer than those of LCT normal group. The ke and t1/2 of MCT showed no statistical difference between normal and ARF groups. In the normal group the ke of LCT was significantly decreased compared with MCT whereas the t1/2 was significantly prolonged. In the ARF group the ke of LCT was much less than that of MCT while the t1/2 was much longer. The serum insulin levels of both MCT groups were significantly higher than those of LCT groups. These results indicate that MCT will be eliminated more rapidly than LCT in ARF rats. MCT may also increase the secretion of insulin. In conclusion, MCT may be more favorable than LCT in the nutritional management of ARF.

Oral Palatability Testing of a Medium-Chain Triglyceride Oil Supplement (MCT) in a Cohort of Healthy Dogs in a Non-Clinical Setting

“…Triglycerides are hydrolysed to release fatty acids, and the rate of metabolism of free fatty acids is very much dependent upon the carbon chain length. Ge et al [26] demonstrated that the maximal reactive rate of lipoprotein lipase hydrolysis on MCT was 2.2 times faster than on long chain triglycerides (LCT), and the reactive rate for the hydrolysis of hepatic triglycerides lipase on MCT was 1.2 times faster compared to LCTs. Given that MCFA have a shorter chain length, they are delivered directly to the liver via the hepatic portal vein to undergo the β-oxidation process [13].…”

Cardio Protective Effects of Lipid Emulsion against Ropivacaine-Induced Local Anesthetic Systemic Toxicity—An Experimental Study

“…One of them is the unknown plasma level of LE or the LA, although the administration was according to international guidelines for lipid rescue in LAST. However, the time interval between LE and LA administration in our setup (2 min) was much shorter than the half-life of long-chain triglycerides, the main constituent of Intralipid administered in rats, which was reported to be more than 30 min [ 22 ]. We can thus safely assume that most of the LE was still present in the circulation at the time Ropivacaine was injected.…”