Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol

Solomon, B. O.; Zeng, An‐Ping; Biebl, Hanno; Schlieker, H.; Posten, Clemens; Deckwer, Wolf‐Dieter

doi:10.1016/0168-1656(94)00148-6

Cited by 59 publications

(43 citation statements)

References 24 publications

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“…The yield coefficients of different products, ATP and biomass can be calculated for these cultures. Table 2 summarizes merely those from the data of Solomon et al [12]. Similar results are obtained with the experimental data of Zeng et al [19].…”

Section: Comparison Of Theoretical Calculations With Experimental Ressupporting

confidence: 87%

“…In conventional continuous culture C. butyricum achieved so far only half of the maximum PD concentration (about 48.5 g/l) obtained with K. pneumoniae [9,11,12]. The reason for this is not clear.…”

Section: Introductionmentioning

confidence: 89%

“…Zeng et al [19] and Solomon et al [12] recently reported on experimental data of glycerol fermentation by C. butyricum in continuous culture under both glycerol limitation (low residua ! glycerol condition) and glycerol excess.…”

Section: Comparison Of Theoretical Calculations With Experimental Resmentioning

confidence: 97%

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Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum

Zeng¹

1996

Bioprocess Engineering

108

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Stoichiometric analysis is applied to continuous glycerol fermentation by Clostridium butyricum to calculate theoretical maximum yields and to predict preferred pathways under different conditions. The upper limits of product concentration and productivity as a function of dilution rate in continuous culture is also predicted from product inhibition kinetic. The theoretical maximum propanediol yield (0.72 mol/mol glycerol) which is calculated for a culture without hydrogen and butyric acid formation agrees well with the experimental maximum value (around 0.71 mol/mol). Comparisons of experimental results (product concentration and productivity) with theoretical calculations and those of the glycerol fermentation by Klebsiella pneumoniae reveal that the production of 1,3-propanediol by C. butyricum is far below the optimum performance available with the present strain. One of the reasons is the relatively high formation of butyric acid under the culture conditions so far applied. The distribution of reducing equivalents to propanediol and hydrogen is also suboptimal. The utilization of the reducing power from pyruvate oxidation for propanediol production is about 60-70% of the theoretical maximum under the present experimental conditions.

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Section: Comparison Of Theoretical Calculations With Experimental Ressupporting

confidence: 87%

Section: Introductionmentioning

confidence: 89%

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Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum

Zeng¹

1996

Bioprocess Engineering

108

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“…Some Klebsiella sp. are fermentative hydrogen-producers (Solomon et al, 1995;Tseng, 2004). A clone closely related to Klebsiella ornithinolytica (similarity 98%) has been found in a hydrogen-producing sludge previously (Iyer et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

Fermentative hydrogen production in packed-bed and packing-free upflow reactors

Zhang

Fang

2006

Water Science and Technology

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Fermentative hydrogen production from a synthetic wastewater containing 10 g/L of sucrose was studied in two upflow reactors at 268C for 400 days. One reactor was filled with packing rings (RP) and the other was packing free (RF). The effect of hydraulic retention time (HRT) from 2 h to 24 h was investigated. Results showed that, under steady state, the hydrogen production rate significantly increased from 0.63 L/L/d to 5.35 L/L/d in the RF when HRT decreased from 24 h to 2 h; the corresponding rates were 0.56 L/L/d to 6.17 L/L/d for the RP. In the RF, the hydrogen yield increased from 0.96 mol/mol-sucrose at 24 h of HRT to the maximum of 1.10 mol/mol-sucrose at 8 h of HRT, and then decreased to 0.68 mol/molsucrose at 2 h. In the RP, the yield increased from 0.86 mol/mol-sucrose at 24 h of HRT to the maximum of 1.22 mol/mol-sucrose at 14 h of HRT, and then decreased to 0.78 mol/mol-sucrose at 2 h. Overall, the reactor with packing was more effective than the one free of packing. In both reactors, sludge agglutinated into granules. The microbial community of granular sludge in RP was investigated using 16S rDNA based techniques. The distribution of bacterial cells and extracellular polysaccharides in hydrogen-producing granules was investigated by fluorescence-based techniques. Results indicated that most of the N-acetylgalactosamine/galactose-containing extracellular polysaccharides were distributed on the outer layer of the granules with a filamentous structure.

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“…The relatively large number of clones affiliated with P. faecium (7 clones out of 36 clones) partly explains the H 2 decline and the moderate propionate accumulation, since these bacteria decarboxylate succinate to propionate through the methylmalonyl CoA pathway (Van Gylswyk, 1995), illustrated by pathway C in Figure 1. The most abundant clones affiliated with K. pneumoniae (26%) are well-known H 2 -producers using the formatecleavage pathway (Axley et al, 1990;Solomon et al, 1995;Yoshida et al, 2006), pathways D and E in Figure 1. The large increase of other fermenting bacteria (i.e., P. faecium and K. pneumoniae), accompanied by the decrease of Clostridium sp., suggest that fermenting bacteria having optimal growth at pH 7 competed well for glucose at initial pH 7.3 (final pH 4.0).…”

Section: Correlation Of the Electron-flow Model With The Bacterial Comentioning

confidence: 97%

An electron‐flow model can predict complex redox reactions in mixed‐culture fermentative BioH₂: Microbial ecology evidence

Lee

Krajmalinik-Brown

Zhang

et al. 2009

Biotech & Bioengineering

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We developed the first model for predicting community structure in mixed-culture fermentative biohydrogen production using electron flows and NADH2 balances. A key assumption of the model is that H2 is produced only via the pyruvate decarboxylation-ferredoxin-hydrogenase pathway, which is commonly the case for fermentation by Clostridium and Ethanoligenens species. We experimentally tested the model using clone libraries to gauge community structures with mixed cultures in which we did not pre-select for specific bacterial groups, such as spore-formers. For experiments having final pHs 3.5 and 4.0, where H2 yield and soluble end-product distribution were distinctly different, we established stoichiometric reactions for each condition by using experimentally determined electron equivalent balances. The error in electron balancing was only 3% at final pH 3.5, in which butyrate and acetate were dominant organic products and the H2 yield was 2.1 mol H2/mol glucose. Clone-library analysis showed that clones affiliated with Clostridium sp. BL-22 and Clostridium sp. HPB-16 were dominant at final pH 3.5. For final pH 4.0, the H2 yield was 0.9 mol H2/mol glucose, ethanol, and acetate were the dominant organic products, and the electron balance error was 13%. The significant error indicates that a second pathway for H2 generation was active. The most abundant clones were affiliated with Klebsiella pneumoniae, which uses the formate-cleavage pathway for H2 production. Thus, the clone-library analyses confirmed that the model predictions for when the pyruvate decarboxylation-ferredoxin-hydrogenase pathway was (final pH 3.5) or was not (final pH 4.0) dominant. With the electron-flow model, we can easily assess the main mechanisms for H2 formation and the dominant H2-producing bacteria in mixed-culture fermentative bioH2.

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Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol

Cited by 59 publications

References 24 publications

Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum

Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum

Fermentative hydrogen production in packed-bed and packing-free upflow reactors

An electron‐flow model can predict complex redox reactions in mixed‐culture fermentative BioH₂: Microbial ecology evidence

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Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol

Cited by 59 publications

References 24 publications

Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum

Pathway and kinetic analysis of 1,3-propanediol production from glycerol fermentation by Clostridium butyricum

Fermentative hydrogen production in packed-bed and packing-free upflow reactors

An electron‐flow model can predict complex redox reactions in mixed‐culture fermentative BioH2: Microbial ecology evidence

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An electron‐flow model can predict complex redox reactions in mixed‐culture fermentative BioH₂: Microbial ecology evidence