Comparison of the effects of silver phosphate and selenium nanoparticles onStaphylococcus aureusgrowth reveals potential for selenium particles to prevent infection

Abstract

Interactions of silver phosphate nanoparticles (SPNPs) and selenium nanoparticles (SeNPs) with Staphylococcus aureus cultures have been studied at the cellular, molecular and protein level. Significant antibacterial effects of both SPNPs and SeNPs on S. aureus were observed. At a concentration of 300 μM, SPNPs caused 37.5% inhibition of bacterial growth and SeNPs totally inhibited bacterial growth. As these effects might have been performed due to the interactions of nanoparticles with DNA and proteins, the interaction of SPNPs or SeNPs with the amplified zntR gene was studied. The presence of nanoparticles decreased the melting temperatures of the nanoparticle complexes with the zntR gene by 23% for SeNPs and by 12% for SPNPs in comparison with the control value. The concentration of bacterial metallothionein was 87% lower in bacteria after application of SPNPs (6.3 μg mg(-1) protein) but was increased by 29% after addition of SeNPs (63 μg mg(-1) protein) compared with the S. aureus control (49 μg mg(-1) protein). Significant antimicrobial effects of the nanoparticles on bacterial growth and DNA integrity provide a promising approach to reducing the risk of bacterial infections that cannot be controlled by the usual antibiotic treatments.

Biogenic selenium nanoparticles synthesized by Stenotrophomonas maltophilia SeITE02 loose antibacterial and antibiofilm efficacy as a result of the progressive alteration of their organic coating layer

“…Despite this variable antimicrobial efficacy of untreated SeNPs towards the different bacterial strains tested, as a general rule MIC values increased with the progressive denaturation of the organic coating layer. These findings suggest that antimicrobial activity of SeNPs is size‐dependent, with higher inhibiting effects associated with the smallest ones, as already reported in previous studies (Lu et al ., 2013; Chudobova et al ., 2014; Zonaro et al ., 2015). Indeed, denaturation of the external organic cap which leads to a progressive increase in particle dimensions coincides with a gradual drop in antibacterial efficacy.…”

Effects of Stratospheric Conditions on the Viability, Metabolism and Proteome of Prokaryotic Cells

“…In terms of biological properties, the effect of UV radiation (laboratory and stratospheric) on the resistance of bacterial strains to environmental externalities, such as conventional antibiotic drugs or their alternative as selenium nanoparticles [24,25], was further monitored. It was shown that the exposure of UV significantly weakened the resistance of bacteria, and the application of antibiotic drugs or nanoparticles became more effective.…”

Complexes of Metal-Based Nanoparticles with Chitosan Suppressing the Risk of Staphylococcus aureus and Escherichia coli Infections

“…Even low concentrations of silver are very effective as a germicidal agent. The antimicrobial effect of silver ions or AgNPs was confirmed by several studies (Chudobova et al, 2013a(Chudobova et al, , b, 2014a.…”

“…Long-term exposure of AgNPs larger than 20 nm to eukaryotic cells may affect them negatively. A combination of AgNPs with a biopolymer like chitosan is a possible solution for its eukaryotic or prokaryotic toxicity (Chudobova et al, 2013b).…”