Comparison of the Effects of Contractubex Gel and Benzothiazole After Topical Application in an Experimental Model of Epidural Fibrosis in Rats

Bozkurt, Hüseyin; Bozkurt, Emel Çiçek; Özpınar, Hülya; Arac, Densel; Kaya, İsmail; Özer, Hıdır; Eğılmez, Reyhan

doi:10.1016/j.wneu.2018.06.044

Cited by 6 publications

(1 citation statement)

References 22 publications

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“…Prior to staining, wells were aspirated and washed again. Two stock solutions of stain SYTO9 and propidium iodide [22] were each diluted in MilliQ water to give a 1 mL:1.5 µL:1.5 µL ratio and 400 µL applied to each well for 15 min before aspirating and washing wells. Live SYTO9-stained cells and dead PI-stained cells were visualised with a confocal laser microscope at 20X magnification, and the percentage of cell death was determined (Zeiss LSM700, Carl Zeiss AG, Oberkochen, Germany).…”

Section: Live/dead Stainingmentioning

confidence: 99%

APTC-EC-2A: A Lytic Phage Targeting Multidrug Resistant E. coli Planktonic Cells and Biofilms

Hon

Camens

et al. 2022

Microorganisms

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Escherichia coli (E. coli) are common bacteria that colonize the human and animal gastrointestinal tract, where they help maintain a balanced microbiome. However, some E. coli strains are pathogenic and can cause serious infectious diseases and life-threatening complications. Due to the overuse of antibiotics and limited development of novel antibiotics, the emergence of antibiotic-resistant strains has threatened modern medicine, whereby common infections can become lethal. Phage therapy has once again attracted interest in recent years as an alternative treatment option to antibiotics for severe infections with antibiotic-resistant strains. The aim of this study was to isolate and characterize phage against multi-drug resistant E. coli isolated from clinical samples and hospital wastewater. For phage isolation, wastewater samples were collected from The Queen Elizabeth Hospital (Adelaide, SA, Australia) followed by phage enrichment as required. Microbiological assays, electron microscopy and genomic sequencing were carried out to characterize the phage. From the 10 isolated E. coli phages, E. coli phage APTC-EC-2A was the most promising and could lyse 6/7 E. coli clinical isolates. APTC-EC-2A was stable at a broad pH range (3–11) and could lyse the host E. coli at temperatures ranging between 30–50 °C. Furthermore, APTC-EC-2A could kill E. coli in planktonic and biofilm form. Electron microscopy and genomic sequencing indicated the phage to be from the Myoviridae family and of lytic nature. In conclusion, the newly isolated phage APTC-EC-2A has the desired properties that support its potential for development as a therapeutic agent against therapy refractory E. coli infections.

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Section: Live/dead Stainingmentioning

confidence: 99%