Comparison of the Effects of Osteochondral Autograft Transplantation With Platelet-Rich Plasma or Platelet-Rich Fibrin on Osteochondral Defects in a Rabbit Model

Maruyama, Masahiro; Satake, Hiroshi; Suzuki, Tomoto; Honma, Ryusuke; Naganuma, Yasushi; Takakubo, Yuya; Takagi, Michiaki

doi:10.1177/0363546517721188

Cited by 25 publications

(48 citation statements)

References 41 publications

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“…Often by passing the in vitro study phase, the pre-clinical implanting of PRF for cartilage repair has been evaluated by several Authors (Table 2). The hemocomponent was tested for the treatment of chondral [62,66,69,70,71,72], osteochondral [53,61,73,74,75,76] or meniscal [67] defects of the knee joint, as well as full-thickness cartilage defects of the ear [77]; the investigated animal models include rabbits [61,62,66,67,69,73,77], pigs [75], dogs [53,70,71,74,76] and horses [72].…”

Section: Prf In Cartilage Tissue Engineeringmentioning

confidence: 99%

“…Although PRF alone has been shown to positively influence cartilage repair in terms of the improvement of macroscopic and histological grading scores [70,71], most studies considered the possibility of implementing its regenerative potential by combining the implantation of the hemocomponent with (a) cartilage granules/fragments [66,69,75], (b) bone marrow-derived MSCs [53,72,74,76,77] and (c) the chemokine Stromal cell-derived factor (SDF)-1 [62]. In addition, PRF implantation was also investigated in association with other therapeutic strategies, such as suture repair of meniscal defects [67] and osteochondral autograft [73].…”

Section: Prf In Cartilage Tissue Engineeringmentioning

confidence: 99%

“…Remarkably, some pre-clinical investigations also reported comparisons between PRF and PRP therapeutic effects on cartilage regeneration, mainly with the aim of verifying whether the fibrin network offered better structural support to cartilage and subchondral bone repair [61,62,71,73].…”

Section: Prf In Cartilage Tissue Engineeringmentioning

confidence: 99%

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Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

Barbon

Stocco

Macchi

et al. 2019

IJMS

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Nowadays, research in Tissue Engineering and Regenerative Medicine is focusing on the identification of instructive scaffolds to address the requirements of both clinicians and patients to achieve prompt and adequate healing in case of injury. Among biomaterials, hemocomponents, and in particular Platelet-rich Fibrin matrices, have aroused widespread interest, acting as delivery platforms for growth factors, cytokines and immune/stem-like cells for immunomodulation; their autologous origin and ready availability are also noteworthy aspects, as safety- and cost-related factors and practical aspects make it possible to shorten surgical interventions. In fact, several authors have focused on the use of Platelet-rich Fibrin in cartilage and tendon tissue engineering, reporting an increasing number of in vitro, pre-clinical and clinical studies. This narrative review attempts to compare the relevant advances in the field, with particular reference being made to the regenerative role of platelet-derived growth factors, as well as the main pre-clinical and clinical research on Platelet-rich Fibrin in chondrogenesis and tenogenesis, thereby providing a basis for critical revision of the topic.

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Section: Prf In Cartilage Tissue Engineeringmentioning

confidence: 99%

Section: Prf In Cartilage Tissue Engineeringmentioning

confidence: 99%

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Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

Barbon

Stocco

Macchi

et al. 2019

IJMS

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“…Em lesões muito extensas, o uso de enxertos osteocondrais íntegros pode se tornar inviável devido à morbidade do local doador. Desta forma, uma alternativa seria a utilização do enxerto osteocondral macerado, que recobre uma área maior e atua como suporte e fonte celular no processo de reparação [10,13,14]. No presente estudo, foram obtidos resultados satisfatórios com a utilização deste tipo de enxerto, associado ou não ao IGF-1.…”

Section: Discussionunclassified

“…A utilização do enxerto osteocondral autógeno possibilita a implantação da cartilagem hialina de forma direta no interior do defeito, em um procedimento único, permitindo o movimento articular precoce e o apoio do membro lesionado [7,14,24]. Alguns fatores de crescimento têm sido avaliados em associação aos tecidos enxertados, tendo destaque para a utilização do fator de crescimento semelhante à insulina tipo 1 (IGF-1), que estimula a proliferação de vários tipos celulares e auxilia na formação de tecido novo [1,11,12,15,16,20,25].…”

Section: Introductionunclassified

Autogenous Osteochondral Graft Associated with IGF-1 in Induced Articular Cartilage Lesion in Rabbits

Kawamoto

Muzzi

Junior

et al. 2018

Acta Scientiae. Vet.

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Background: Articular cartilage has a limited capacity for regeneration and of the various treatments proposed, none have reached appropriate therapeutic effectiveness. This study aimed to evaluate autogenous osteochondral grafts in intact or macerated format, in association with or without insulin-like growth factor type-1 (IGF-1) in the repair of osteochondral defects induced in the femoral trochlear groove of rabbits.Materials, Methods & Results: Seventeen healthy White New Zealand rabbits were selected for this study. The rabbits were female, six months old, and had an average body weight of 4.5 kg. All 34 stifle joints were subjected to autogenous osteochondral grafting in the femoral trochlear groove. The joints were divided into four groups designated as intact osteochondral graft with IGF-1 (INT + IGF), intact osteochondral graft with physiological solution (INT + FIS), macerated osteochondral graft with IGF-1 (MAC + IGF), and macerated osteochondral graft with physiological solution (MAC + FIS). Serial evaluations were performed by orthopedic and radiographic examination. After 6 and 12 weeks postoperatively, the grafted area was subjected to macroscopic, histological, and immunohistochemical analyses. Although no statistically significant differences were found between the groups in relation to clinical, macroscopic, histological, and immunohistochemical aspects, a tendency of IGF-1 to promote tissue repair was evident. In the radiographic evaluation, the articular surface and the recipient site in both groups with IGF-1 showed significantly more effective filling (P ≤ 0.05). Regardless of the group, collagen type 2 production, as assessed by immunohistochemistry, was found to be appropriate on the grafted articular surface.Discussion: In extensive cartilage lesions, the use of intact osteochondral grafts may be infeasible due to donor site morbidity. An alternative is the use of macerated osteochondral grafts, which cover a larger area and act as a support and cellular source in the repair process. Growth factors have been evaluated in association with grafted tissues to aid tissue repair, and IGF-1 is currently prominent. In the radiographic analysis of the present study, when comparing sites subjected to osteochondral grafting, presence of the whole graft evidenced adequate local filling in all groups. However, graft integration was apparently rapid and effective in the INT + IGF and MAC + IGF groups from the sixth and ninth weeks of the procedure, respectively. In the macroscopic evaluation at the twelfth week, graft integration with the original cartilaginous tissue was more evident, especially in both groups treated with IGF-1. It is likely that the property of IGF-1 to increase chondrogenesis in the cartilage repair of articular lesions in vivo may have contributed to these results in radiographic and macroscopic examinations. Histological examination showed no significant difference between groups in the same period of time; however, it was observed that addition of IGF-1 promoted a more evident tissue reaction and cellular activation, potentiating the process of reabsorption and repair in the grafted tissue. Immunohistochemical analysis showed similar immunoreactivity for collagen type 2 in all groups as early as the sixth week. However, a small portion of these tissues cannot be considered true hyaline cartilage due to the absence of some typical features. In summary, addition of IGF-1 to the autogenous osteochondral graft seemed to stimulate reabsorption and replacement processes in the grafted tissue. The grafts showed adequate ability to repair articular cartilage, displaying formation of collagen type 2 similar to that in the original tissue.

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Cartilage Injury and Repair

Li¹,

Wang²,

Liu³

et al. 2022

Biofabrication for Orthopedics

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Comparison of the Effects of Osteochondral Autograft Transplantation With Platelet-Rich Plasma or Platelet-Rich Fibrin on Osteochondral Defects in a Rabbit Model

Abstract: PRF has the potential to improve clinical outcomes of OAT used to treat osteochondral lesions.

Cited by 25 publications

References 41 publications

Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

Platelet-Rich Fibrin Scaffolds for Cartilage and Tendon Regenerative Medicine: From Bench to Bedside

Autogenous Osteochondral Graft Associated with IGF-1 in Induced Articular Cartilage Lesion in Rabbits

Cartilage Injury and Repair

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