Comparison of the effects of spaceflight and hindlimb-suspension on rat pituitary vasopressin and brainstem norepinephrine content

Fareh, Jeannette; S, Fagette; Cottet-Émard, J. M.; Allevard, A. M.; Viso, Michel; Gauquelin, G.; Gharib, Claude

doi:10.1016/0273-1177(94)90424-3

Cited by 2 publications

(1 citation statement)

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“…In vivo studies of the effects of microgravity on the female reproductive system of rodents are limited to the antiorthostatic suspension model, which was primarily developed for the study of skeletal and muscular systems. The use of this model for the reproductive system is possible [10,40], in particular, as a less stressful effect than space flight [41]. However, additional validation of structural and functional changes in the reproductive system of females during suspension and in real space flight is required.…”

Section: Limitations Of the Studymentioning

confidence: 99%

The Oxidative Phosphorylation and Cytoskeleton Proteins of Mouse Ovaries after 96 Hours of Hindlimb Suspension

Gorbacheva,

Sventitskaya,

Biryukov

et al. 2023

Life

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The purpose of this study was to assess oxidative phosphorylation (OXPHOS) in mouse ovaries, determine the relative content of proteins that form the respiratory chain complexes and the main structures of the cytoskeleton, and determine the mRNA of the corresponding genes after hindlimb suspension for 96 h. After hindlimb suspension, the maximum rate of oxygen uptake increased by 133% (p < 0.05) compared to the control due to the complex I of the respiratory chain. The content of mRNA of genes encoding the main components of the respiratory chain increased (cyt c by 78%, cox IV by 56%, ATPase by 69%, p < 0.05 compared with the control). The relative content of cytoskeletal proteins that can participate in the processes of transport and localization of mitochondria does not change, with the exception of an increase in the content of alpha-tubulin by 25% (p < 0.05) and its acetylated isoform (by 36%, p < 0.05); however, the mRNA content of these cytoskeletal genes did not differ from the control. The content of GDF9 mRNA does not change after hindlimb suspension. The data obtained show that short-term exposure to simulated weightlessness leads to intensification of metabolism in the ovaries.

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Section: Limitations Of the Studymentioning

confidence: 99%