Comparison of the effects of four anaesthetics on biochemical blood profiles of perch

Abstract

This study investigated the feasibility of using clove oil, 2-phenoxyethanol, or Propiscin as an alternative to tricaine methane sulphonate (MS 222) as a ¢sh anaesthetic, particularly in regard to reducing ¢sh stress. The biochemical blood pro¢les of perch Perca £uviatilis L. anaesthetized with either MS 222 (100 mg L À 1 ), clove oil (33 mg L À 1 ), 2-phenoxyethanol (0.40 mL L À 1 ) or Propiscin (1.0 mL L À 1 ), and a non-anaesthetized control group were compared. Biochemical pro¢les were determined from blood samples collected before treatment in controls. For each anaesthetic tested, ¢sh were divided into two groups, one sampled immediately after 10-min anaesthesia and a second, sampled 24 h after 10-min anaesthesia. The values determined in the present study suggested that internal organs and tissues of perch were slightly altered by MS 222, clove oil and 2-phenoxyethanol anaesthesia, but not by Propiscin anaesthesia.

The effects of four anaesthetics on haematological and blood biochemical profiles in vimba bream, Vimba vimba

“…Velisek and Svobodova (2004a) and Velisek et al (2005a) reported decreased AST activity in rainbow trout after clove oil and 2-phenoxyethanol anaesthesia. A similar change in LDH activity was reported by Velisek et al (2009) with clove oil anaesthesia in perch. Velisek et al (2011) reported increased activity of AST with clove oil and 2-phenoxyethanol anaesthesia in rainbow trout.…”

“…Gomulka et al (2008) also reported decreased ammonia levels in Siberian sturgeon (Acipenser baerii) after eugenol (0.075 ml/l) and MS 222 (125 mg/l) anaesthesia. No changes in the levels of ammonia in rainbow trout, carp, European catfish, or perch following clove oil (30 mg/l) and 2-phenoxyethanol (0.30 ml/l) anaesthesia were observed by Velisek and Svobodova (2004a), and Velisek et al (2005bVelisek et al ( , 2007Velisek et al ( , 2009). Our differing results may be associated with the concentrations of the tested substances and the fish species used.…”

“…The analysis of blood parameters is one of the most valuable methods available for modern diagnostics (Anver Celik 2004), and can provide important information about the internal environment of the organism (Anver Celik 2004;Velisek et al 2005aVelisek et al ,b, 2006Velisek et al , 2009Velisek et al , 2011Kristan et al 2012). Haematological and biochemical profiles are frequently used for evaluation of the effect of anaesthetics (Iwama et al 1989;Velisek and Svobodova 2004a,b;Velisek et al 2005aVelisek et al ,b, 2006Velisek et al , 2007Velisek et al , 2009Velisek et al , 2011Kristan et al 2012).…”

“…Haematological and biochemical profiles are frequently used for evaluation of the effect of anaesthetics (Iwama et al 1989;Velisek and Svobodova 2004a,b;Velisek et al 2005aVelisek et al ,b, 2006Velisek et al , 2007Velisek et al , 2009Velisek et al , 2011Kristan et al 2012). To our knowledge, no other data on biochemical and haematological profiles in vimba bream anaesthetized with MS 222, Propiscin, 2-phenoxyethanol, or clove oil are available.…”

“…A variety of anaesthetics with differing properties have been used in aquaculture (Cho and Heath 2000;Kazun and Siwicki 2001;Velisek and Svobodova 2004a,b;Velisek et al 2005aVelisek et al ,b, 2006Velisek et al , 2007Velisek et al , 2009Velisek et al , 2011. Chemicals used in aquaculture are subject to strict control, particularly with regard to safety and efficacy (Taylor and Roberts 1999).…”