Comparison of the effects of multiwall carbon nanotubes on the epithelial cells and macrophages

Horie, Masanori; Tabei, Yosuke; Sugino, Sakiko; Fukui, Hitoshi; Nishioka, Ayako; Hagiwara, Yoshimichi; Sato, Kei; Yoneda, Tetsuro; Tada, Atsumi; Koyama, Tamami

doi:10.1080/17435390.2019.1592258

Cited by 12 publications

(14 citation statements)

References 36 publications

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“…S6). In contrast to the present study, NRCWE-006 (Mitsui-7) did not induce IL-8 expression in A549 cells exposed to MWCNT, when these were dispersed in Pluronic [35]. Different dispersion media have been shown to affect the dispersion state and ultimately the endpoints in vitro [55] and in vivo [56], which might explain some diverging results.…”

Section: Discussioncontrasting

confidence: 99%

“…We assessed the pro-inflammatory response in vitro by quantifying expression levels of IL-8, a neutrophil chemoattractant highly expressed during inflammation in both human and murine cells (mouse homologue gene is Cxcl-1), as a result of the NF-kβ inflammatory pathway [35,36]. IL-8 mRNA levels correlate with IL-8 protein levels in A549 cells [26].…”

Section: Discussionmentioning

confidence: 99%

“…To assess the pro-inflammatory response and correlate to neutrophil influx in vivo, we quantified gene expression of the neutrophil chemo-attractant interleukin-8 (IL-8, also known as C-X-C motif chemokine ligand 8 (CXCL8)). IL-8 is an early proinflammatory biomarker and highly expressed during NM-induced inflammation in both human and murine cells (mouse homologue gene is Cxcl-1), as a result of the NF-kβ inflammatory pathway [35][36][37][38]. IL-8 mechanistic relevance has been further indicated in Adverse Outcome Pathways frameworks [26].…”

Section: Introductionmentioning

confidence: 99%

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In vitro-in vivo correlations of pulmonary inflammogenicity and genotoxicity of MWCNT

et al. 2021

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Background Multi-walled carbon nanotubes (MWCNT) have received attention due to extraordinary properties, resulting in concerns for occupational health and safety. Costs and ethical concerns of animal testing drive a need for in vitro models with predictive power in respiratory toxicity. The aim of this study was to assess pro-inflammatory response (Interleukin-8 expression, IL-8) and genotoxicity (DNA strand breaks) caused by MWCNT with different physicochemical properties in different pulmonary cell models and correlate these to previously published in vivo data. Seven MWCNT were selected; two long/thick (NRCWE-006/Mitsui-7 and NM-401), two short/thin (NM-400 and NM-403), a pristine (NRCWE-040) and two surface modified; hydroxylated (NRCWE-041) and carboxylated (NRCWE-042). Carbon black Printex90 (CB) was included as benchmark material. Human alveolar epithelial cells (A549) and monocyte-derived macrophages (THP-1a) were exposed to nanomaterials (NM) in submerged conditions, and two materials (NM-400 and NM-401) in co-cultures of A549/THP-1a and lung fibroblasts (WI-38) in an air-liquid interface (ALI) system. Effective doses were quantified by thermo-gravimetric-mass spectrometry analysis (TGA-MS). To compare genotoxicity in vitro and in vivo, we developed a scoring system based on a categorization of effects into standard deviation (SD) units (< 1, 1, 2, 3 or 4 standard deviation increases) for the increasing genotoxicity. Results Effective doses were shown to be 25 to 53%, and 21 to 57% of the doses administered to A549 and THP-1a, respectively. In submerged conditions (A549 and THP-1a cells), all NM induced dose-dependent IL-8 expression. NM-401 and NRCWE-006 caused the strongest pro-inflammatory response. In the ALI-exposed co-culture, only NM-401 caused increased IL-8 expression, and no DNA strand breaks were observed. Strong correlations were found between in vitro and in vivo inflammation when doses were normalized by surface area (also proxy for diameter and length). Significantly increased DNA damage was found for all MWCNT in THP-1a cells, and for short MWCNT in A549 cells. A concordance in genotoxicity of 83% was obtained between THP-1a cells and broncho-alveolar lavaged (BAL) cells. Conclusion This study shows correlations of pro-inflammatory potential in A549 and THP-1a cells with neutrophil influx in mice, and concordance in genotoxic response between THP-1a cells and BAL cells, for seven MWCNT.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro-in vivo correlations of pulmonary inflammogenicity and genotoxicity of MWCNT

et al. 2021

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“…Obviously, in vitro systems never fully recapitulate the in vivo situation but nevertheless can be very useful for hazard ranking. Indeed, the NR8383 cell line has also proven to be useful for (nano)particle hazard grouping strategies, showing good comparability with outcomes from in vivo studies [ 44 , 45 , 46 ]. To evaluate the suitability of the NR8383 cells for the investigation of the inflammasome activating properties of the TiO 2 NMs, at first, experiments were performed in the absence of LPS priming.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the NLRP3 Inflammasome Activating Effects of a Large Panel of TiO2 Nanomaterials in Macrophages

et al. 2020

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TiO2 nanomaterials are among the most commonly produced and used engineered nanomaterials (NMs) in the world. There is controversy regarding their ability to induce inflammation-mediated lung injuries following inhalation exposure. Activation of the NACHT, LRR and PYD domains-containing protein 3 (NALP3) inflammasome and subsequent release of the cytokine interleukin (IL)-1β in pulmonary macrophages has been postulated as an essential pathway for the inflammatory and associated tissue-remodeling effects of toxic particles. Our study aim was to determine and rank the IL-1β activating properties of TiO2 NMs by comparing a large panel of different samples against each other as well as against fine TiO2, synthetic amorphous silica and crystalline silica (DQ12 quartz). Effects were evaluated in primary bone marrow derived macrophages (BMDMs) from NALP3-deficient and proficient mice as well as in the rat alveolar macrophage cell line NR8383. Our results show that specific TiO2 NMs can activate the inflammasome in macrophages albeit with a markedly lower potency than amorphous SiO2 and quartz. The heterogeneity in IL-1β release observed in our study among 19 different TiO2 NMs underscores the relevance of case-by-case evaluation of nanomaterials of similar chemical composition. Our findings also further promote the NR8383 cell line as a promising in vitro tool for the assessment of the inflammatory and inflammasome activating properties of NMs.

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“…Previous studies also indicate that certain MWCNTs can result in increased oxidative stress and inflammation which play vital roles in PF. Pro-inflammatory cytokines such as interleukin-8 (IL-8) are secreted from both the epithelium and resident immune cells in response to MWCNT exposure [ 15 , 16 ]. IL-8 is a potent neutrophil chemoattractant that is elevated in PF [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Walled Carbon Nanotubes (MWCNTs) Cause Cellular Senescence in TGF-β Stimulated Lung Epithelial Cells

Lucas

Wang

Muthumalage

et al. 2021

Toxics

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Multi-walled carbon nanotubes are engineered nanomaterials (ENMs) that have a fiber-like structure which may be a concern for the development of cellular senescence. Premature senescence, a state of irreversible cell cycle arrest, is implicated in the pathogenesis of chronic lung diseases such as pulmonary fibrosis (PF). However, the crosstalk between downstream pathways mediating fibrotic and senescent responses of MWCNTs is not well-defined. Here, we exposed human bronchial epithelial cells (BEAS-2B) to MWCNTs for up to 72 h and demonstrate that MWCNTs increase reactive oxygen species (ROS) production accompanied by inhibition of cell proliferation. In addition, MWCNT exposure resulted in the increase of p21 protein abundance and senescence associated β-galactosidase (SA β-gal) activity. We also determined that co-exposure with the cytokine, transforming growth factor-β (TGF-β) exacerbated cellular senescence indicated by increased protein levels of p21, p16, and γH2A.X. Furthermore, the production of fibronectin and plasminogen activator inhibitor (PAI-1) was significantly elevated with the co-exposure compared to MWCNT or TGF-β alone. Together, our study suggests that the cellular senescence potential of MWCNTs may be enhanced by pro-fibrotic mediators, such as TGF-β in the surrounding microenvironment.

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Comparison of the effects of multiwall carbon nanotubes on the epithelial cells and macrophages

Cited by 12 publications

References 36 publications

In vitro-in vivo correlations of pulmonary inflammogenicity and genotoxicity of MWCNT

In vitro-in vivo correlations of pulmonary inflammogenicity and genotoxicity of MWCNT

Evaluation of the NLRP3 Inflammasome Activating Effects of a Large Panel of TiO2 Nanomaterials in Macrophages

Multi-Walled Carbon Nanotubes (MWCNTs) Cause Cellular Senescence in TGF-β Stimulated Lung Epithelial Cells

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