Comparison of the Effects between Tannins Extracted from Different Natural Plants on Growth Performance, Antioxidant Capacity, Immunity, and Intestinal Flora of Broiler Chickens

Abstract

In this study, four plant tannins, including AT (Acacia mearnsii tannin, 68%), CT (Castanea sativa tannin, 60%), QT (Schinopsis lorenzii tannin, 73%) and TT (Caesalpinia spinosa tannin, 50%) were added to broiler diets for 42 days to evaluate and compare their effects on growth performance, antioxidant capacity, immune performance and gut microbiota in broilers. The results showed that the supplementation of five tannins could increase the production of T-AOC, GSH-Px, SOD and CAT and reduce the production of MDA in the serum of broilers (p < 0.01), but the antioxidant effect of the AT group was lower than that of the other three groups (p < 0.01). All four tannins decreased the level of the pro-inflammatory factor IL-1β and increased the level of the anti-inflammatory factor IL-10 (p < 0.01). CT, QT and TT decreased the levels of pro-inflammatory factors IL-6 and TNF-α (p < 0.01), while AT and CT increased the level of IL-2 in serum (p < 0.01). Supplementation with four tannins also increased the levels of IgG, IgM, IgA and sIgA in serum (p < 0.01) and the levels of ZO-1, claudin-1 and occludin in the jejunum (p < 0.01). The detection results of ALT and AST showed that CT, QT and TT decreased the concentrations of ALT and AST in serum (p < 0.01). The results of the gut microbiota showed that the abundance of Clostridia and Subdoligranulum increased, and the abundance of Oscillospiraceae decreased, compared to the control group after adding the four tannins to the diets (p > 0.05). In addition, CT, QT and TT decreased the abundance of Lactobacillus and increased the abundance of Bacteroides compared to the control group, while AT showed the opposite result (p > 0.05). Overall, our study shows that tannins derived from different plants have their own unique effects on broilers. AT and CT can promote broilers’ growth better than other tannins, CT has the best ability to improve immune and antioxidant properties, and QT and TT have the best effect on broilers’ liver protection.

Effect of Tannic Acid on Antioxidant Function, Immunity, and Intestinal Barrier of Broilers Co-Infected with Coccidia and Clostridium perfringens

“…This study found that jejunal pro-inflammatory mediators (IL-8, IL-1β, iNOS, IFN-γ) were significantly upregulated, the anti-inflammatory mediator (IL-10) was significantly downregulated in broilers with the CCP challenge, and these results suggested that CCP-infected broilers elicited an intestinal inflammatory response in the jejunum. In our study, dietary supplementation with 1000 mg/kg TA significantly diminished pro-inflammatory mediators and elevated anti-inflammatory mediators with CCP challenge, consistent with previous studies [ 43 , 44 , 45 ]. These results suggest that dietary supplementation with 1000 mg/kg TA is beneficial in alleviating intestinal inflammatory responses in broilers co-infected with CCP.…”

Combination of Hydrolysable Tannins and Zinc Oxide on Enterocyte Functionality: In Vitro Insights

“…Our investigation found that the ZnO/HTs treatment preserved the cellular expression and localization of tight junction proteins ZO-1 and OCLN. This finding is consistent with various in vivo and in vitro studies concerning HTs, tannic acid, and other polyphenolic compounds [20,49,50]. Conversely, existing literature on ZnO primarily focuses on nanoparticle formulations, which may induce increased cellular cytotoxicity [51].…”

The effect of Phyllanthus emblica (Amla) fruit supplementation on the rumen microbiota and its correlation with rumen fermentation in dairy cows

“…The relative abundance of the family Oscillospiraceae increased significantly at 400 g/d FAF feeding dose, and it was the only potential biomarker identified for this treatment group ( Figures 7A , B ). Liu et al (2023) discovered that different tannin sources added to broiler diets had varying effects on Oscillospiraceae, with enrichment observed only in Castanea sativa tannin and Schinopsis lorentzii tannin; these specific tannins degrade various structural carbohydrates and produce butyrate, positively impacting broilers’ liver protection. Furthermore, Aurantii Fructus immaturus flavonoid extract increased the proportion of Oscillospiraceae and ameliorated colitis induced by dextran sulfate sodium in mice ( Chen S-.Y.…”