Comparison of the effectiveness of polyethylenimine, polyamidoamine and chitosan in transferring plasmid encoding interleukin-12 gene into hepatocytes

Dehshahri, Ali; Alhashemi, Samira Hossaini; Jamshidzadeh, Akram; Sabahi, Zahra; Mohammadi-Samani, Soliman; Sadeghpour, Hossein; Mohazabieh, Erfaneh; Fadaei, M.

doi:10.1007/s13233-013-1180-9

Cited by 34 publications

(38 citation statements)

References 51 publications

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“…However, tight complexes are formed by wraparound of pDNA leading to the more protective effects. [8][9][10][11][12][13] As the concentration of nuclease in animal or human body is lower than that of DNase I used in this study (0.25 units), the administration of the polyplexes even at a C/P ratio of 0.25 might lead to protection at the physiological condition.…”

mentioning

confidence: 86%

“…Among the different polycationic compounds used in transfection, polyethylenimine (PEI) has been considered as the most extensively investigated cationic polymer due to its ability to interact with the negatively charged backbone of the nucleic acids and form nano-sized particles. [7][8][9] The characteristics of PEI are highly related to the high amine content, which causes a significant positive charge density on the surface of the polymer. 10,11 Thus, PEI can interact with the negatively charged components of the cell membrane, which results in its translocation into the cell.…”

Section: Introductionmentioning

confidence: 99%

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Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene

Nouri¹,

Sadeghpour²,

Heidari³

et al. 2017

IJN

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Various strategies have been utilized to improve both gene transfer efficiency and cell-induced toxicity of polyethylenimine (PEI), the most extensively investigated cationic polymeric vector. In this study, we sought to enhance transfection efficiency of low molecular weight PEI (LMW PEI) while maintaining its low toxicity by cross-linking LMW PEI via succinic acid linker. These modifications were designed to improve the hydrophilic–hydrophobic balance of the polymer, by enhancing the buffering capacity and maintaining low cytotoxic effects of the final conjugate. Decreased expression of CD200 in the central nervous system has been considered as one of the proposed mechanisms associated with neuroinflammation in multiple sclerosis; therefore, we selected plasmid-encoding CD200 gene for transfection using the modified PEI derivatives. Dynamic light scattering experiments demonstrated that the modified PEIs were able to condense plasmid DNA and form polyplexes with a size of approximately 130 nm. The highest level of CD200 expression was achieved at a carrier to plasmid ratio of 8, where the expression level was increased by 1.5 fold in the SH-SY5Y cell line, an in vitro model of neurodegenerative disorders. Furthermore, the results of in vivo imaging of the LMW PEI-based nanoparticles in the mouse model of multiple sclerosis revealed that fluorescently labeled plasmid encoding CD200 was distributed from the injection site to various tissues and organs including lymph nodes, liver, brain, and finally, kidneys. The nanoparticles also showed the ability to cross the blood–brain barrier and enter the periventricular area.

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mentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene

Nouri¹,

Sadeghpour²,

Heidari³

et al. 2017

IJN

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“…The preparation of succinylated PEI was performed according to our previous investigation. 21,22 Briefly, succinic anhydride was added dropwise to the 25 kDa PEI solution and incubated at room temperature. The dialysis (10,000 Da cutoff Spectra/Por membrane) was carried out against NaCl solution (0.25 M) followed by twice against water at 4 C. The fluffy materials obtained following the lyophilization were characterized by 1 H-NMR (D 2 O) spectroscopy using a Bruker Avance DRX-500 MHz NMR spectrometer (Bruker, Ettlingen, Germany).…”

Section: Synthesis Of Bpei Derivativesmentioning

confidence: 99%

Targeted double domain nanoplex based on galactosylated polyethylenimine enhanced the delivery of IL‐12 plasmid

Dehshahri

Sadeghpour

Mohazzabieh

et al. 2020

Biotechnology Progress

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The objective of the present investigation was to design a targeted polyethylenimine (PEI)-based polyplex by conjugating lactose bearing galactose groups on low molecular weight PEI (LMW PEI) grafted to a high molecular weight PEI (HMW PEI) via a succinic acid linker in order to restore the amine content of the whole conjugate used for ligand conjugation. The PEI conjugate was synthesized and characterized in terms of buffering capacity, particle size, zeta potential, plasmid condensation ability, and protection of DNA against degrading enzymes. Also, the transfection efficiency and cytotoxicity were evaluated in the cell line over-expressing asialoglycoprotein receptors (ASGPRs) and compared with the cells lacking the receptors. The results demonstrated the ability of PEI conjugate in condensation of plasmid DNA and protection against enzyme degradation. The PEI conjugate formed nanoparticles of around 75 nm with higher buffering capacity compared with unmodified PEI. The polyplexes prepared by the modified PEI could increase the level of transgene up to four folds in the cells over-expressing the receptor. The results demonstrated the separation of targeting and delivery domains could be considered as a strategy to restore the amine content of the PEI molecule utilized for targeting ligand conjugation.

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“…Completed correction of the memory defect in an experimental model of AD [139] PLGA functionalized with OX26-type monoclonal antibody TMZ Cytotoxicity improvement of TMZ [140] PLGA-b-PEG-ascorbic acid Galantamine (GLM) High biodistribution, therapeutic, and sustained action of the drug [141] [44,[163][164][165][166][167][168][169][170][171][172]. Among them, polyesters have great potential because of their biocompatibility and biodegradability.…”

Section: Aβ Generation Inhibitor and Curcuminmentioning

confidence: 99%

“…Synthetic polymers have been widely used for the delivery of bioactive agents and drugs [44,[163][164][165][166][167][168][169][170][171][172]. Among them, polyesters have great potential because of their biocompatibility and biodegradability.…”

Section: Polyestersmentioning

confidence: 99%

Multifunctional Polymeric Nanoplatforms for Brain Diseases Diagnosis, Therapy and Theranostics

et al. 2020

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The blood–brain barrier (BBB) acts as a barrier to prevent the central nervous system (CNS) from damage by substances that originate from the blood circulation. The BBB limits drug penetration into the brain and is one of the major clinical obstacles to the treatment of CNS diseases. Nanotechnology-based delivery systems have been tested for overcoming this barrier and releasing related drugs into the brain matrix. In this review, nanoparticles (NPs) from simple to developed delivery systems are discussed for the delivery of a drug to the brain. This review particularly focuses on polymeric nanomaterials that have been used for CNS treatment. Polymeric NPs such as polylactide (PLA), poly (D, L-lactide-co-glycolide) (PLGA), poly (ε-caprolactone) (PCL), poly (alkyl cyanoacrylate) (PACA), human serum albumin (HSA), gelatin, and chitosan are discussed in detail.

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Comparison of the effectiveness of polyethylenimine, polyamidoamine and chitosan in transferring plasmid encoding interleukin-12 gene into hepatocytes

Cited by 34 publications

References 51 publications

Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene

Preparation, characterization, and transfection efficiency of low molecular weight polyethylenimine-based nanoparticles for delivery of the plasmid encoding CD200 gene

Targeted double domain nanoplex based on galactosylated polyethylenimine enhanced the delivery of IL‐12 plasmid

Multifunctional Polymeric Nanoplatforms for Brain Diseases Diagnosis, Therapy and Theranostics

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