Comparison of the effectiveness of dipping agents on bacteria causing mastitis in cattle

Abstract

Introduction. Mastitis may result in physical, chemical and microbiological changes in milk and pathological lesions in the glandular tissue. Milk derived from cows with mastitis may become a cause of infections in humansw and animals. Objectives. The aim of this study was to assess the effectiveness of selected dipping agents in the inactivation of several bacteria that may cause mastitis in cattle. Materials and method. Three strains of each of the following species: Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Staphylococcus aureus and Listeria monocytogenes, isolated from milk, were used in the study. Identification of isolates was carried out using the automatic system VITEK2 Compact. Evaluation of the genetic similarity between the tested strains was made using the RAPD technique. Drug susceptibility of strains was evaluated with the disc diffusion method. Assessment of the effectiveness of iodine, stabilized iodine, povidone iodine and chlorhexidine was performed using fragments of skin from cow teats. Results. All the tested strains were genetically different. Most of them were susceptible to the studied antibiotics. Only two strains of L. monocytogenes were resistant to all the studied antibiotics. The percentage rate of reduction in the number of bacteria after using of dipping agents was very high (>90%). The most susceptible to the dipping preparations used were L. monocytogenes (99.6-99.9%). Stabilized iodine was the most effective dipping agent for all tested bacteria, causing a reduction rate in the number of bacteria from 99.80% (E. coli)-99.99% (S. aureus, L. monocytogenes). Conclusions. The results obtained may contribute to a reduction in udder infections in cows, especially mastitis, and improve the quality of the milk.

Characterization of Escherichia coli Strains Derived from Cow Milk of Subclinical and Clinical Cases of Mastitis

“…There were no significant differences in the bactericidal efficacy between the dipping agents. This supports our previous study showing the high effectiveness of all three chemicals against E. coli, S. aureus, L. monocytogenes, and S. marcescens [17]. Additionally, Pelletier et al [36] showed high effectiveness of preparations based on iodine at various concentrations in teat dip.…”

“…The efficacy of dipping substances was assessed on 30 E. coli strains of cows with a clinical form of mastitis and 30 E. coli strains of cows with subclinical mastitis. The evaluation of effectiveness of dipping agents was previously described by Skowron et al [17]. Fragments of the teat skin of slaughtered cows were used.…”

“…both between the animals and to the milk tank. One way to minimalize the risk of BM is an efficient teat predipping and postdipping, aiming to destroy the microorganisms on the udder skin and in a drop of the milk of the teat canal [17]. Dipping agents commonly applied for teat disinfection are iodine and its derivatives, and chlorhexidine.…”

Impacts of Milking and Housing Environment on Milk Microbiota

“…This correlation may easily be overlooked. A previous study demonstrated that ineffectiveness of dipping agents, dirty udders and incomplete teat dip cup cleaning are among the highest risk factors for bacterial contamination in farms [35]. Incomplete cleaning and disinfection of milk liners and teat dipping cups with automatic milking systems in dairy farms resulted in the relatively high prevalence of these pathogens in Galicia [36].…”

Infectious aetiologies of subclinical bovine mastitis and antimicrobial susceptibility on northwest of Algeria

“…Antibiotic therapy and vaccination are the commonly used methods for the treatment of mastitis, and belong to several modes of conventional and advanced therapeutic measures, which contribute to the reduction of intramammary infections in cows, and also aid in improvement of the milk quality (Skowron et al, 2019). Extensive and uncontrolled use of antibiotics for the treatment, along with the induction and persistence of biofilm-associated antibiotic resistance in mastitis, has led to the decreased response to antibiotic therapy (Park et al, 2012;Babra et al, 2013).…”