Comparison of the Effectiveness of Autologous Fibrin Glue and Macroporous Biphasic Calcium Phosphate as Carriers in the Osteogenesis Process With or Without Mesenchymal Stem Cells

Lee, Li-Tzu; Kwan, Po-Cheung; Chen, Ya-Fang; Wong, Yong–Kie

doi:10.1016/s1726-4901(08)70077-7

Cited by 27 publications

(21 citation statements)

References 31 publications

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“…Fibrin is a natural biopolymer that has been used alone or in combination with other materials to generate bone tissue successfully . Fibrin has been paired with more mechanically stable materials such as bioceramics in bone tissue engineering; however, fibrin has also been investigated in the form of a fibrin hydrogel or fibrin glue as a carrier for cells in cell‐based therapy …”

Section: Discussionmentioning

confidence: 99%

Osteoprogenitor Cell Therapy in an Equine Fracture Model

et al. 2012

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Objective To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model. Study Design Experimental. Animals Adult horses (n = 10). Methods Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses. There was 1 treatment and 1 control limb in each horse. Bone defects were randomly injected with either fibrin glue and osteoprogenitor cells or fibrin glue alone. Radiography was performed every week until the study endpoint at 12 weeks. After euthanasia, bone healing was evaluated using radiography and histology. Analysis of radiographic data was conducted using a linear‐mixed model. Analysis of histologic data was conducted using a general linear model. Statistical significance was set at P < .05. Results Radiographic grayscale data as a measure of bone healing revealed no significant difference between treatment and control limbs. Radiographic scoring results also showed that the treatment effect was not significant. Histologic analysis was consistent with radiographic analysis showing no significant difference between the area of bone present in treatment and control limbs. Conclusion Injection of periosteal‐derived osteoprogenitors in a fibrin glue carrier into surgically created ostectomies of MC4 does not accelerate bone healing when compared with fibrin glue alone.

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Section: Discussionmentioning

confidence: 99%

Osteoprogenitor Cell Therapy in an Equine Fracture Model

et al. 2012

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“…Because they are natural materials, fibrin sealants are completely biocompatible [30]. A number of authors have investigated fibrin for tissue engineering applications and demonstrated successful growth and proliferation of encapsulated MSCs [31] [32] [33] and myoblasts [34]. However, the main drawback to this class of adhesives is a low cohesive strength [35] [36].…”

Section: Introductionmentioning

confidence: 99%

Thermogelling bioadhesive scaffolds for intervertebral disk tissue engineering: Preliminary in vitro comparison of aldehyde-based versus alginate microparticle-mediated adhesion

et al. 2015

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Tissue engineering of certain load-bearing parts of the body can be dependent on scaffold adhesion or integration with the surrounding tissue to prevent dislocation. One such area is the regeneration of the intervertebral disc (IVD). In this work, poly(N-isopropylacrylamide) (PNIPAAm) was grafted with chondroitin sulfate (CS) (PNIPAAm-g-CS) and blended with aldehyde-modified CS to generate an injectable polymer that can form covalent bonds with tissue upon contact. However, the presence of the reactive aldehyde groups can compromise the viability of encapsulated cells. Thus, liposomes were encapsulated in the blend, designed to deliver the ECM derivative, gelatin, after the polymer has adhered to tissue and reached physiological temperature. This work is based on the hypothesis that the discharge of gelatin will enhance the biocompatibility of the material by covalently reacting with, or “end-capping”, the aldehyde functionalities within the gel that did not participate in bonding with tissue upon contact. As a comparison, formulations were also created without CS aldehyde and with an alternative adhesion mediator, mucoadhesive calcium alginate particles. Gels formed from blends of PNIPAAm-g-CS and CS aldehyde exhibited increased adhesive strength compared to PNIPAAm-g-CS alone (p<0.05). However, the addition of gelatin-loaded liposomes to the blend significantly decreased the adhesive strength (p<0.05). The encapsulation of alginate microparticles within PNIPAAm-g-CS gels caused the tensile strength to increase two-fold over that of PNIPAAm-g-CS blends with CS aldehyde (p<0.05). Cytocompatibility studies indicate that formulations containing alginate particles exhibit reduced cytotoxicity over those containing CS aldehyde. Overall, the results indicated that the adhesives composed of alginate microparticles encapsulated in PNIPAAm-g-CS have the potential to serve as a scaffold for IVD regeneration.

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“…104 Fibrin glue encapsulation alone likely imparts a benefit in the delivery and retention of cells within the scaffold or defect. 48,105,106 In previous studies demonstrating a benefit for PRP and MSCs in combination but not in comparison with other delivery methods or encapsulation substrates, 107,108 the benefit may be due in large part to the role of the PRP as a delivery vehicle for the MSCs, 109 given the otherwise short-term survival of seeded MSCs. 110 The need for a cell delivery vehicle may be dependent on the type of scaffold used; in a study by Haasper et al, no benefit of cellular delivery by fibrin glue to scaffolds to which cells adhere more efficiently, such as bone matrix, has been demonstrated.…”

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confidence: 99%

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Kretlow

Spicer

Jansen

et al. 2010

Tissue Engineering Part A

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For bone tissue engineering, the benefits of incorporating mesenchymal stem cells (MSCs) into porous scaffolds are well established. There is, however, little consensus on the effects of or need for MSC handling ex vivo. Culture and expansion of MSCs adds length and cost, and likely increases risk associated with treatment. We evaluated the effect of using uncultured bone marrow mononuclear cells (bmMNCs) encapsulated within fibrin glue hydrogels and seeded into porous scaffolds to regenerate bone over 12 weeks in an 8-mm-diameter, criticalsized rat cranial defect. A full factorial experimental design was used to evaluate bone formation within model poly(L-lactic acid) and corraline hydroxyapatite scaffolds with or without platelet-rich plasma (PRP) and bmMNCs. Mechanical push-out testing, microcomputed tomographical analyses, and histology were performed. PRP showed no benefit for bone formation. Cell-laden poly(L-lactic acid) scaffolds without PRP required significantly greater force to displace from surrounding tissues than control (cell-free) scaffolds, but no differences were observed during push-out testing of coral scaffolds. For bone volume formation as analyzed by microcomputed tomography, significant positive overall effects were observed with bmMNC incorporation. These data suggest that bmMNCs may provide therapeutic advantages in bone tissue engineering applications without the need for culture, expansion, and purification.

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Comparison of the Effectiveness of Autologous Fibrin Glue and Macroporous Biphasic Calcium Phosphate as Carriers in the Osteogenesis Process With or Without Mesenchymal Stem Cells

Abstract: Using stem cells plus autologous fibrin glue as the carrier may accelerate new bone regeneration.

Cited by 27 publications

References 31 publications

Osteoprogenitor Cell Therapy in an Equine Fracture Model

Osteoprogenitor Cell Therapy in an Equine Fracture Model

Thermogelling bioadhesive scaffolds for intervertebral disk tissue engineering: Preliminary in vitro comparison of aldehyde-based versus alginate microparticle-mediated adhesion

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

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