Comparison of the effect of activated or non-activated PRP in various concentrations on osteoblast and fibroblast cell line proliferation

Abstract

Platelet-rich plasma (PRP) contains growth factors which positively affect cell proliferation, cell differentiation, chemotaxis and intracellular matrix synthesis. All these processes are involved in wound healing and tissue regeneration; thus, PRP as a source of growth factors can be used in periodontal regenerative therapies. The purpose of the present study was to assess the effect of various concentrations of activated and non-activated PRP on proliferation of osteoblasts and fibroblasts in vitro. PRP was obtained from three healthy volunteers. 75, 50, 25, and 10% concentrations of f PRP were prepared by dilution in Dulbecco's modified Eagle's medium. In activated PRP groups, PRP concentrations were activated by adding calcium gluconate. Human gingival fibroblast (HGF) cell line and MG-63 (osteosarcoma) human osteoblast-like cell line were used in the study. The MTT proliferation assay was used to assess the effect of different types of PRP concentrates on proliferation of HGF and MG-63 cells, in 24, 48 and 72 h. After 24, 48, and 72 h, the proliferation rate of both cell lines was higher in the positive control group, except in 72 h in HGF cell lines, that 10% non-activated PRP group and 10 and 25% activated PRP groups has higher proliferation rate than the positive control group, which it was not significant. Proliferation rate in cells with 10% activated PRP was highest among samples containing PRP. The current study failed to show the significant effect of activated or non-activated PRP on proliferation of HGFs or MG-63 osteoblast-like cells. However, our results showed that activated PRP had a greater effect than non-activated PRP.

Effects of platelet-rich plasma and platelet-poor plasma on human dermal fibroblasts

“…Longer PRP evaluation periods may be required as fibroblast cell inhibition occurred between 0 to 24h, and on day seven fibroblast proliferation achieved statistical significance [16]. Another study realised no significance occurred in fibroblast proliferation from 24 to 72-h after the co-culture of PRP, and at this time the higher concentrations exhibited cell inhibition [50]. To the contrary, another study displayed that high concentrations (0.3-5 x 10 6 plt/l) did not cause cell inhibition for up to 72-h and achieved the greatest proliferation up to 2 x 10 6 plt/l.…”

Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

“…For this purpose, many physicians prefer to activate PRP using various activation methods, such as use of calcium chloride, collagen, thrombin, calcium chloride/thrombin mixture, or freeze/thaw cycle . However, these methods are associated with concerns of early washout of the released growth factors, prompting other physicians to use non‐activated PRP, thereby relying on native collagen at the desired site to promote activation, resulting in slow growth factor release . To clarify the effect of activation on growth factor release, we evaluated the growth factor concentration with and without activation in the present study.…”

“…25,27 However, these methods are associated with concerns of early washout of the released growth factors, JOURNAL OF ORTHOPAEDIC RESEARCH ® APRIL 2020 prompting other physicians to use non-activated PRP, thereby relying on native collagen at the desired site to promote activation, resulting in slow growth factor release. 20,[22][23][24] To clarify the effect of activation on growth factor release, we evaluated the growth factor concentration with and without activation in the present study. In accordance with the findings of Oh et al, 26 our study showed that some degree of growth factor existed before the intentional exogenous activation of PRP.…”

“…However, others prefer it without activation as ex vivo activation is associated with concerns of early washout of the released growth factors, whereas PRP activation by the native collagen of connective tissue in vivo results in slow release of growth factors. [20][21][22][23][24] Thus, to clarify the effect of PRP, it is important to evaluate growth factor concentration with and without activation under different storage conditions.…”

Platelet‐rich plasma, a powerful tool in dermatology

“…Vahabi et al made this comparison using calcium gluconate as an activator, by means of cell proliferation determination (of osteoblasts and fibroblasts in vitro ). They observed that in the function of time, activated PRP had a higher effect on cell proliferation than nonactivated PRP, although these results were not statistically significant (Vahabi, Yadegari, & Mohammad‐Rahimi, ).…”