Comparison of the Effect of Low-Level Laser and Phenytoin Therapy on Skin Wound Healing in Rats

Taheri, Jamileh Bigom; Bagheri, Fatemeh; Mojahedi, M; Shamloo, Nafiseh; Nakhostin, Mohammad Reza; Azimi, Somayyeh; Namazi, Zahra

doi:10.15171/jlms.2015.06

Cited by 8 publications

(14 citation statements)

References 21 publications

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“…However, considering the other outcomes in current study such as MTT analysis showing the viability of the cells, it is highly probable that these cells are proliferating rather that dying. In line with other reports showing that light energy aided wound‐healing , our results suggest that LEDs exert such an effect.…”

Section: Discussionsupporting

confidence: 92%

“…On morphological analysis, the actin level (as revealed by phalloidin staining) increased in the irradiation group (Figure A), as did the length of the major axis (Figure B) (unpaired t test, t = 3.846, df = 31, P = .0006), but not that of the minor axis or the cellular area. Thus, LED irradiation enhancing wound‐healing , increased fibroblast activity, and altered cell morphology.…”

Section: Resultsmentioning

confidence: 99%

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Light‐emitting diode irradiation using 660 nm promotes human fibroblast HSP90 expression and changes cellular activity and morphology

Choi

Chang

Biswas

et al. 2019

Journal of Biophotonics

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We evaluated changes in cell viability and morphology in response to low‐level light irradiation and underlying variations in the levels of heat shock proteins (HSPs). Human fibroblasts were irradiated with a light‐emitting diode (LED) array at 660 nm (50 mW for 15, 30, and 60 minutes). Cell viability and morphological changes were evaluated via epifluorescence analysis; we also assessed cell viability and length changes. The expression levels of adenosine triphosphate (ATP) and various HSPs (HSP27, 60, 70, and 90) were analyzed by immunohistochemical staining, Western blotting and microarray analysis. After LED irradiation, cellular viability and morphology changed. Of the several HSPs analyzed, the HSP90 level increased significantly, suggesting that this protein played roles in the morphological and cellular changes. Thus, low‐level irradiation triggered cellular changes mediated by increased HSP90 expression; this may explain why skin irradiation enhances wound‐healing.

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Section: Discussionsupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Light‐emitting diode irradiation using 660 nm promotes human fibroblast HSP90 expression and changes cellular activity and morphology

Choi

Chang

Biswas

et al. 2019

Journal of Biophotonics

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“…1 In humans, axons are generally on the scale of 1 μm thick and some are more than 1 m long, 1 and in the peripheral nerve system (PNS), they are either unmyelinated or myelinated by Schwann cells. 2 These cells are mainly wrapped around axons and form a multi-layered sheath preparing myelin structures for axons. 2,3 In case of injury, Schwann cells are activated and play a key role in nerve repair mechanism, not only concerning Wallerian degeneration and remyelination in the injured position, but also in promoting axonal growth.…”

Section: Introductionmentioning

confidence: 99%

“…2 These cells are mainly wrapped around axons and form a multi-layered sheath preparing myelin structures for axons. 2,3 In case of injury, Schwann cells are activated and play a key role in nerve repair mechanism, not only concerning Wallerian degeneration and remyelination in the injured position, but also in promoting axonal growth. 4 Wallerian degeneration occurs when an axon is cut or injured, in which the axon degenerates distal to the injury site.…”

Section: Introductionmentioning

confidence: 99%

Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat

et al. 2017

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IntroductionThe nerve fiber or axon, is responsible for transmitting information between neuron cells. 1 In humans, axons are generally on the scale of 1 μm thick and some are more than 1 m long, 1 and in the peripheral nerve system (PNS), they are either unmyelinated or myelinated by Schwann cells.2 These cells are mainly wrapped around axons and form a multi-layered sheath preparing myelin structures for axons. 2,3 In case of injury, Schwann cells are activated and play a key role in nerve repair mechanism, not only concerning Wallerian degeneration and remyelination in the injured position, but also in promoting axonal growth. 4 Wallerian degeneration occurs when an axon is cut or injured, in which the axon degenerates distal to the injury site. [5][6][7] This is followed by secondary phenomena such as breakdown of the myelin sheet and activation of the Schwann and macrophages that clear up the debris. 8,9Nerve injuries are divided into 3 basic categories of neuropraxia, axonotmesis and neurotmesis based on the extent of injury, pathology and physiology according to Sir Herbert Seddon's scheme, 5,6 neuropraxia, the mildest nerve injury, is a temporary loss of sensory function. 10The injured site usually fully recovers within few weeks or months, and no Wallerian degeneration occurs in this type of injury. Axonotmesis is when axons are more seriously damaged and interrupted. 10 In this type of injury, the surrounding epineurium and perineurium layers remain almost intact, but there could be only partial recovery depending on the extent of injury to the axons. In addition, Wallerian degeneration may occur distal to Methods:In this research work, the effect of LLLT (780 nm) on the regeneration process and reconstruction of injured peripheral right side sciatic nerve was investigated. Twelve adult male Wistar rats underwent surgery in aseptic conditions under general anesthesia to induce a lesion to their right side sciatic nerve according to standard protocol. Before suturing the location, only the experimental group was treated by laser. The damaged nerve was directly irradiated with (2 J, 100 mW, 40 seconds). The irradiation procedure was terminated in 21 days with little improvement (4 J, 200 mW, 40 seconds) across the skin surface of experimental group. Rats were selected randomly from each group to be sacrificed on different periods and histopathological examination was carried out on the extracted nerves.Results: Significant acceleration of revascularization and angiogenesis of the injury site was observed in the experimental group. Furthermore, a reduction of hemorrhages and increase in blood supply was observed. Also, Wallerian degeneration decreased while higher axonal density compared to the control rats was observed. Moreover, the cross-section analysis of the injured area on the 14th and 21st days as post-surgery showed that the nerve sheath diameter in the lesion area of the experimental group was reduced. While the ratio between thicknesses increased in the control group. Conclusion:The results of...

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“…7,11 In previous studies, several modalities have been introduced for acceleration of wound healing, such as traditional medicine 12,13 and chemical drugs like phenytoin. 14 Laser therapy, introduced in 1976 for wound healing, 15 is proposed not only as a modality to relieve pain 16 but also for its healing promotion effects. 14,17,18 The exact mechanism underlying laser efficacy in accelerated wound healing has not been demonstrated yet.…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of the Efficacy of Laser Therapy and Fibroblastic Growth Factor Injection on Mucosal Wound Healing in Rat Experimental Model

et al. 2018

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Introduction: The aim of the present study was to compare the effects of laser and basic fibroblastic growth factor (bFGF) treatment on operative wound healing in a rat model. Methods: Sixty-six male Wistar rats were employed in this study. A 10-mm surgical wound was created on the buccal mucosa of each rat, under anesthesia, and then the rats were divided into 3 groups of 22: (1) GF group (received subcutaneous injection of bFGF), (2) laser group (treated with low-level laser irradiation), and (3) control group (received no treatment). On day 5, half of the rats in each group and on day 10 the other half, were sacrificed. Afterward, samples were taken from rats' buccal mucosa for histological assay and scoring. The data were analyzed using Mann-Whitney test (α = 5%). Results: On day 5 there was not any significant difference between GF and control groups; however, the laser group showed clinically delayed wound coverage, compared to other groups (P < 0.05). On day 10, histological examination demonstrated marked vascular granulation tissue (GT) in GF group. Collagen production was significantly prominent in laser group compared to GF treated samples (P = 0.004). Inflammation of GT in GF and laser groups was significantly less than that in control samples (P = 0.005 and P = 0.001, respectively). Conclusion: The components of wound matrix induced by GF and laser treatment were significantly different. Although bFGF or laser treatment of oral wounds, under the conditions of the present study, did not accelerate wound healing, they showed some other notable effects on the quality of healing.

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Comparison of the Effect of Low-Level Laser and Phenytoin Therapy on Skin Wound Healing in Rats

Cited by 8 publications

References 21 publications

Light‐emitting diode irradiation using 660 nm promotes human fibroblast HSP90 expression and changes cellular activity and morphology

Light‐emitting diode irradiation using 660 nm promotes human fibroblast HSP90 expression and changes cellular activity and morphology

Stimulation Effect of Low Level Laser Therapy on Sciatic Nerve Regeneration in Rat

Comparative Evaluation of the Efficacy of Laser Therapy and Fibroblastic Growth Factor Injection on Mucosal Wound Healing in Rat Experimental Model

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