Comparison of the developmental stages of some European allocreadiid trematode species and a clarification of their life-cycles based on ITS2 and 28S sequences

Abstract

Genetic data were used to examine the diversity in some allocreadiid trematodes. Nuclear ribosomal DNA (ITS2 and partial sequences of 5.8S and 28S) was sampled from sexual adult and 'larval' stages. From these and previous reference datasets phylogenetic trees were constructed. The results uncovered genetically distinct lineages within Bunodera luciopercae (Müller, 1776), suggesting that the two Palaearctic subspecies, B. l. luciopercae and B. l. acerinae Roitman & Sokolov, 1999, and Nearctic B. luciopercae from Perca flavescens may represent distinct species with a restricted host-specificity. Identical rDNA was revealed for the sexual adult of B. l. acerinae and 'larval' B. luciopercae described by Wiśniewski (1958). An unexpected match between the rDNA sequences of adult B. l. luciopercae and 'larval' Allocreadium isoporum (sensu Wiśniewski, 1958) was also detected. The adult A. isoporum (Looss, 1894) differs significantly from the 'larval' A. isoporum, the level of rDNA sequence divergence between them (8.6 % for 5.8S-ITS2-28S sequences and 6.26% for 28S) being consistent with the level expected for intergeneric variation. These results revealed the possible existence of a cryptic species complex within the nominal species B. luciopercae and a clear need for reconsideration of some of the accepted, but largely untested, tenets regarding allocreadiid life-cycles.

Genetic characterization of far eastern species of the genus <i>Crepidostomum</i> (Trematoda: Allocreadiidae) by means of 28S ribosomal DNA sequences

“…auriculatum not so far. Such genetic stability irrespective of the host species was shown for other trematode species which infect different species of animals [35][36][37]. Moreover, C. cf.…”

DNA barcodes show cryptic diversity and a potential physiological basis for host specificity among Diplostomoidea (Platyhelminthes: Digenea) parasitizing freshwater fishes in the St. Lawrence River, Canada

“…2000; Poulin & Keeney 2007; Steinauer et al. 2007; Petkevičiūtė et al 2010). To our knowledge, this approach has not been applied previously to trematode metacercariae and few molecular surveys of any helminth parasites have been conducted on a comparable scale.…”

“…2005, 2006; Poulin & Keeney 2007; Steinauer et al. 2007; Petkevičiūtė et al 2010), but this trend has not emerged from molecular studies of diplostomoids (Table S1, Supporting information).…”

Diversity and phylogenetic relationships of European species of Crepidostomum Braun, 1900 (Trematoda: Allocreadiidae) based on rDNA, with special reference to Crepidostomum oschmarini Zhokhov & Pugacheva, 1998

“… Sphaerium corneum Ukraine: River Belka, Dnieper River basin GU462121 [ 44 ] FJ874919 [ 44 ] Allocreadium sp. a Pisidium amnicum Russia: River Tvertsa, upper Volga River basin FJ874923 [ 44 ] Allocreadium gotoi Misgurnus anguillicaudatus Japan: Nagano, Iiyama, Midori LC215274 [ 61 ] Allocreadium isoporum Alburnus alburnus Russia: Lake Oster, Karelia GU462125, GU462126 [ 44 ] FJ874921 [ 44 ] Allocreadium isoporum Barbatula barbatula Russia: River Il’d, upper Volga River basin MH143102 MH143096 Allocreadium lobatum Semotilus corporalis USA: Moosehead Lake, Maine EF032693 [ 62 ] Allocreadium neotenicum Hydroporus rufifrons United Kingdom: Lake District, Cumbria JX977132 [ 43 ] Allocreadium neotenicum Oreodytes sanmarkii Norway: Lake Takvatn KY513133 [ 18 ] Allocreadium neotenicum a Pisidium casertanum Ukraine: River Burulcha, Crimea MH143103 MH143075 Allocreadium neotenicum a P. casertanum Norway: Lake Takvatn MH143104 MH143076 Allocreadium neotenicum a Pisidium sp. Norway: Lake Nordersjoen MH143105 MH143077 Auriculostoma sp.…”