Comparison of the Continuous Mononuclear Cell and Mononuclear Cell Protocols on the Spectra Optia Apheresis System for Collecting Autologous and Allogeneic Peripheral Blood Stem Cells (PBSCs)

Abstract

Mobilization failure rates were <5% across all thresholds. Mobilization costs were comparable. Conclusion: We demonstrate that a liberal strategy for plerixafor administration correlates with fewer apheresis days while having similar mobilization costs. There were 26 fewer days of collection per 100 mobilization attempts with a pCD34 threshold of 40/μL compared to 15/μL at our institution. More patients completed collection in one day, and more reached an optimal collection yield.

Impact of custom prime on the PBSC collection efficiency and procedure outcome on Spectra Optia apheresis device by using the CMNC program in low-weight pediatric oncology patients. A single-center experience

“…Moreover, CMNC protocol had higher predictability of cell yield concerning the pre-apheresis CD34+ cells [2,4], higher throughput [5] (collection rate of cells per time), and shorter run times with lower and more predictable product volume [2][3][4]. On the other hand, the higher product hematocrit [2] and higher maximal extracorporeal volume (ECV) of CMNC (297 ml) versus one in MNC (191 ml), could be seen as the potential drawbacks, but both disadvantages can be managed relatively easy. According to literature, the RBC in product never exceeded 20 ml 2 , which is suitable even in allogeneic settings and, custom prime with allogeneic blood (BP) of the extracorporeal circuit (ECC) can effectively prevent severe hemodynamic changes.…”

“…The CMNC protocol in pediatrics is still of limited experience, but in practice, it showed comparable [2,3] or even higher [4] collection efficiency (CE) in comparison with MNC. Moreover, CMNC protocol had higher predictability of cell yield concerning the pre-apheresis CD34+ cells [2,4], higher throughput [5] (collection rate of cells per time), and shorter run times with lower and more predictable product volume [2][3][4]. On the other hand, the higher product hematocrit [2] and higher maximal extracorporeal volume (ECV) of CMNC (297 ml) versus one in MNC (191 ml), could be seen as the potential drawbacks, but both disadvantages can be managed relatively easy.…”