Comparison of the ColiPlate™ Kit with Two Common E. coli Enumeration Methods for Water

Gibson, Cassi J.; Maritim, Abraham K.; Marion, Jason W.

doi:10.3390/w13131804

Cited by 5 publications

(6 citation statements)

References 40 publications

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“…Previous studies have shown the efficacy of defined substrate technologies (DST) and their reliability in testing for E. coli in water samples. DSTs are known to be comparably sensitive to traditional membrane filtration methods [46], and similar results have been observed using ColiPlates™ [47,48]. Further, defined substrate detection and enumeration methods for coliforms and E. coli have been widely accepted by various regulatory and scientific entities [48,49].…”

Section: Enumeration Of Escherichia Coli Across Field Sitesmentioning

confidence: 57%

“…DSTs are known to be comparably sensitive to traditional membrane filtration methods [46], and similar results have been observed using ColiPlates™ [47,48]. Further, defined substrate detection and enumeration methods for coliforms and E. coli have been widely accepted by various regulatory and scientific entities [48,49]. ColiPlate™ kits enumerate and quantify E. coli abundance but do not differentiate between species, as is the case with traditional membrane filtration methods used in recreational water monitoring.…”

Section: Enumeration Of Escherichia Coli Across Field Sitesmentioning

confidence: 62%

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Examining the potential impacts of a coastal renourishment project on the presence and abundance of Escherichia coli

Lewis,

Frost,

Heard

2024

PLoS ONE

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Erosion poses a significant threat to oceanic beaches worldwide. To combat this threat, management agencies often utilize renourishment, which supplements eroded beaches with offsite sand. This process can alter the physical characteristics of the beach and can influence the presence and abundance of microbial communities. In this study, we examined how an oceanic beach renourishment project may have impacted the presence and abundance of Escherichia coli (E. coli), a common bacteria species, and sand grain size, a sediment characteristic that can influence bacterial persistence. Using an observational field approach, we quantified the presence and abundance of E. coli in sand (from sub-tidal, intertidal, and dune zones on the beach) and water samples at study sites in both renourished and non-renourished sections of Folly Beach, South Carolina, USA in 2014 and 2015. In addition, we also measured how renourishment may have impacted sand grain size by quantifying the relative frequency of grain sizes (from sub-tidal, intertidal, and dune zones on the beach) at both renourished and non-renourished sites. Using this approach, we found that E. coli was present in sand samples in all zones of the beach and at each of our study sites in both years of sampling but never in water samples. Additionally, we found that in comparison to non-renourished sections, renourished sites had significantly higher abundances of E. coli and coarser sand grains in the intertidal zone, which is where renourished sand is typically placed. However, these differences were only present in 2014 and were not detected when we resampled the study sites in 2015. Collectively, our findings show that E. coli can be commonly found in this sandy beach microbial community. In addition, our results suggest that renourishment has the potential to alter both the physical structure of the beach and the microbial community but that these impacts may be short-lived.

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Section: Enumeration Of Escherichia Coli Across Field Sitesmentioning

confidence: 57%

Section: Enumeration Of Escherichia Coli Across Field Sitesmentioning

confidence: 62%

Examining the potential impacts of a coastal renourishment project on the presence and abundance of Escherichia coli

Lewis,

Frost,

Heard

2024

PLoS ONE

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“…These tests were developed and validated in a narrow range of water types, despite being the standard of practice, so it is also possible that environmental bacteria in Lake Victoria waters could result in false positives, as well. However, they do serve as an overall relative proxy for human health risk (Ramoutar, 2020;Gibson et al, 2021).…”

Section: Laboratory Analysesmentioning

confidence: 99%

Local-scale impacts of water hyacinth on water quality in a hypereutrophic lake

et al. 2023

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Increased nutrient concentrations have been linked to water quality impairments across the world. In Lake Victoria, the second largest freshwater lake in the world, decades of eutrophication have challenged water quality management and led to conditions with compromising levels of cyanobacteria and cyanotoxins. Our study focuses on Winam Gulf, where a resurgence of water hyacinth has led to renewed challenges with navigation and anoxia, but also may present a local-scale opportunity for water quality improvements. Over the course of 1.5 years, we conducted three, in situ experiments to vary levels of water hyacinth density and determined its effects on lake water nutrient composition, biochemical parameters (chlorophyll a, cyanotoxins), and fecal indicators (fecal coliforms and Escherchia coli). While our study did not show overwhelming improvements in water quality with increasing water hyacinth density, we did find that small-scale removal of water hyacinth led to decreases in coliforms (F1, 11 = 11.14, p < 0.01), E. coli (F1, 11 = 22.95, p < 0.001), and total microcystins (F1, 9 = 3.47, p = 0.095). Throughout the experiment, the majority of samples collected had both E. coli and microcystin concentrations, notably exceeding World Health Organization guidelines for drinking water. Our data suggest that magnitude of scale, as well as season considerations such as rainfall and background level of water hyacinth, will strongly influence the phytoremediation potential of water hyacinth. The data encourages further exploration of remediation potential of native and introduced macrophytes.

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“…Second, water quality is evaluated by the "most probable number" method which is based on the growth of target organisms in a liquid medium and the subsequent calculation of the "most probable number" (MPN) of organisms with reference to MPN tables. This approach for quantifying is used in the Bluewater Biosciences ColiPlate™ kit [6]. In addition, the US Environmental Protection Agency (EPA)-approved Colilert Quanti-Tray is used to monitor the presence or absence of coliforms and E. coli, as well as to quantify their levels within 18 to 21 h [http://www.idexx.de/water/water-testing-solutions.html] (accessed on 25 October 2021) [6].…”

Section: Introductionmentioning

confidence: 99%

“…This approach for quantifying is used in the Bluewater Biosciences ColiPlate™ kit [6]. In addition, the US Environmental Protection Agency (EPA)-approved Colilert Quanti-Tray is used to monitor the presence or absence of coliforms and E. coli, as well as to quantify their levels within 18 to 21 h [http://www.idexx.de/water/water-testing-solutions.html] (accessed on 25 October 2021) [6].…”

Section: Introductionmentioning

confidence: 99%

A Model System for Sensitive Detection of Viable E. coli Bacteria Combining Direct Viability PCR and a Novel Microarray-Based Detection Approach

et al. 2021

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We established an innovative approach that included direct, viability, and nested PCR for rapid and reliable identification of the fecal indicator organism Escherichia coli (E. coli). Direct PCR enabled successful amplification of the target uidA gene, omitting a prior DNA isolation or purification step. Furthermore, we applied viability PCR (v-PCR) to ensure the detection of only relevant viable bacterial cells. The principle involves the binding of propidium monoazide (PMA), a selective nucleic acid intercalating dye, to accessible DNA of heat killed bacteria cells and, consequently, allows viable and heat killed E. coli cells to be discriminated. To ensure high sensitivity, direct v-PCR was followed by a nested PCR step. The resulting amplicons were analyzed by a rapid 30 min microarray-based DNA hybridization assay for species-specific DNA detection of E. coli. A positive signal was indicated by enzymatically generated silver nanoparticle deposits, which served as robust endpoint signals allowing an immediate visual readout. The presented novel protocol allows the detection of 1 × 101 viable E. coli cells per PCR run.

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Comparison of the ColiPlate™ Kit with Two Common E. coli Enumeration Methods for Water

Cited by 5 publications

References 40 publications

Examining the potential impacts of a coastal renourishment project on the presence and abundance of Escherichia coli

Examining the potential impacts of a coastal renourishment project on the presence and abundance of Escherichia coli

Local-scale impacts of water hyacinth on water quality in a hypereutrophic lake

A Model System for Sensitive Detection of Viable E. coli Bacteria Combining Direct Viability PCR and a Novel Microarray-Based Detection Approach

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