Comparison of the class 1 outer membrane proteins of eight serological reference strains of <i>Neisseria meningitidis</i>

Maiden, Martin C. J.; Suker, Janet; McKenna, A. J.; Bygraves, Jane A.; Feavers, Ian M.

doi:10.1111/j.1365-2958.1991.tb00743.x

Cited by 113 publications

(123 citation statements)

References 30 publications

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“…This region corresponded to loop 7 of the FetA structural model and was previously shown to contain the epitopes for several mouse mAbs (van der . The localization of most variation in an area corresponding to a putative surface-exposed loop on the b-barrel structure of an OMP was redolent of the two major VRs found in the meningococcal PorA porin (Maiden et al, 1991;McGuinness et al, 1990McGuinness et al, , 1993.…”

Section: Discussionmentioning

confidence: 97%

Antigenic diversity of meningococcal enterobactin receptor FetA, a vaccine component

2003

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Meningococcal FetA (FrpB), an iron-regulated outer-membrane protein and vaccine component, was shown to be highly diverse: a total of 60 fetA alleles, encoding 56 protein sequences, were identified from 107 representative Neisseria meningitidis isolates. Phylogenetic analysis established that the allelic variants had been generated by both point mutation and horizontal genetic exchange. Nucleotide substitution was unevenly distributed in the gene, which contained both conserved and variable sequence regions. The most conserved region of the translated peptide sequence corresponded to an amino-terminal domain of the protein and the most diverse region to a previously identified variable region (VR). A nomenclature system for the peptides encoded by the VR was devised which classified 24 variants into 5 FetA variant families. On the basis of these data, murine polyclonal sera specific for four FetA variants were generated. The reactivities of these sera in whole-cell ELISA experiments were consistent with the hypothesis that the VR encoded an immunodominant epitope and indicated that the sera reacted mainly with variants against which they were raised. The diversity of this protein is likely to limit its effectiveness as a vaccine component.

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Section: Discussionmentioning

confidence: 97%

Antigenic diversity of meningococcal enterobactin receptor FetA, a vaccine component

2003

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“…The polymerase chain reactions (PCRs) were performed as described by Maiden P t c11., 13 except that the concentration of the primers was 0 . 2 p~ to prevent nonspecific binding.…”

Section: Dot-blotting and Immunoblottingmentioning

confidence: 99%

Expression of an inaccessible P1.7 subtype epitope on meningococcal class 1 proteins

Wedege¹,

Dalseg²,

Caugant³

et al. 1993

Journal of Medical Microbiology

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Summary. Dot-blot analysis of whole-cell suspensions of meningococci showed that 8 1 O h of B : 15 : P1.16 strains from patients reacted with a monoclonal antibody (MAb) against subtype PI .7. The remaining strains, which did not react on dot-blots or in ELISA, demonstrated the P1.7 subtype epitope on immunoblots after denaturation of the cells with sodium dodecyl sulphate. The monomeric class 1 proteins of the two P1.16 subtype variants had slightly different mol. wts, but bound the PI .7 antibody equally well. These results were explained by a deletion of three codons in the gene encoding the first variable region of the P 1.16 class 1 protein. The deletion accounted for the non-exposure of the P1.7 epitope on native cells. Other patient strains, with subtypes P1.3, P1.9 or without any known subtype, also showed a binding site for the P1.7 MAb, which became available only after denaturation. Demonstration of inaccessible epitopes may have consequences for subtype designations and vaccine development.

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“…The advent of identification libraries derived from sequence-based typing systems that utilize non-selective housekeeping genes, such as the multilocus sequence typing (MLST) schemes (Maiden et al, 1998;Spratt, 1999) (http:// www.mlst.net), has facilitated the ability to identify and track particular strains over time. Other markers that may be under selective evolutionary pressure, including those encoding antibiotic resistance (Oliveira et al, 2001) and surface proteins (Maiden et al, 1991;Gaia et al, 2003), can also be used, particularly in clonal organisms such as Bordetella pertussis, where the selection of useful markers can be problematic.…”

Section: Introductionmentioning

confidence: 99%

Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK

Packard

Parton

Coote

et al. 2004

Journal of Medical Microbiology

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To determine the value of gene markers for surveillance and to assess the genetic stability of potential acellular pertussis vaccine components, the sequence variation in ten virulence-related genes of Bordetella pertussis was investigated in strains isolated in the UK between 1920 and 2002. These genes encode: pertactin (prnA); pertussis toxin subunits S1 (ptxA) and S3 (ptxC); tracheal colonization factor (tcfA); bordetella autotransporter protein C (bapC); bordetella resistance to killing protein (brkA); fimbrial antigen 2 (fim2); outer-membrane protein Q (ompQ); virulence-activated gene 8 (vag8) and adenylate cyclase toxin (cyaA). The encoded proteins are either components of current acellular vaccines (ACVs), or potential virulence markers for B. pertussis. Three strains used in the pertussis UK whole-cell vaccine (WCV), strain Tohama-I used for production of ACV components and the type strain of B. pertussis (18323 T ) were also analysed. Several novel alleles were found. The UK isolates were assigned multi-locus sequence types (MLSTs) according to a previously described scheme for B. pertussis based on three of these genes (ptxA, ptxC and tcfA). Compared with isolates from other countries, the UK clinical strains showed a distinct distribution of MLSTs. Apart from one strain that was MLST-3, all other recent isolates (2000)(2001)(2002) were identified as MLST-5. These isolates differed from the three WCV strains, which were MLST-2 or MLST-3, the Tohama-I strain (MLST-2) and the type strain of B. pertussis (MLST-9). MLST-3 and MLST-5 differ only by a single synonymous mutation, but this method does indicate that currently circulating strains of B. pertussis are not identical to the vaccine types, and they may differ in other important characteristics. Two new MLSTs were identified amongst historical UK isolates. Sequence-based typing offers a convenient method of analysing and comparing populations of B. pertussis from different time periods and from different countries. The variation exhibited by prnA and fim2 suggests that they could be useful, additional epidemiological markers in such a typing scheme.

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Comparison of the class 1 outer membrane proteins of eight serological reference strains of Neisseria meningitidis

Cited by 113 publications

References 30 publications

Antigenic diversity of meningococcal enterobactin receptor FetA, a vaccine component

Antigenic diversity of meningococcal enterobactin receptor FetA, a vaccine component

Expression of an inaccessible P1.7 subtype epitope on meningococcal class 1 proteins

Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK

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