Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of <i>Salvia miltiorrhiza</i> Bge. between sweating and nonsweating

Li, Guozhuan; Fan, Ying; Wang, Yanyan; Yao, Liang; Qiu, Zhen; Wang, Ting; Wang, Zhiqiang; Yang, Fumeng; Peng, Daiyin; Yu, Nianjun; Chen, Weidong

doi:10.1002/bmc.4203

Cited by 19 publications

(11 citation statements)

References 52 publications

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“…Its active compounds are tanshinone I, tanshinone IIA, cryptotanshinone, salvianolic acid B, protocatechuic aldehyde, etc. [ 43 ]. It has been reported to have an anti-inflammatory effect [ 44 ], an anxiolytic effect [ 45 ], and anti-cancer effects [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Activation of ER Stress-Dependent miR-216b Has a Critical Role in Salvia miltiorrhiza Ethanol-Extract-Induced Apoptosis in U266 and U937 Cells

Kim

Song

Park

et al. 2018

IJMS

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Although Salvia miltiorrhiza has been reported to have anti-cancer mechanisms, such as caspase activation, cell cycle arrest, an anti-angiogenesis effect, and Bcl-2 family regulation, its underlying mechanism of endoplasmic reticulum (ER) stress-mediated apoptosis has never been demonstrated. Thus, in this current study, ER stress-related apoptosis via miR-216b of the ethanol extract of Salvia miltiorrhiza (SM) is elucidated for the first time. SM treatment inhibited the viability of U266 and U937 cells in a concentration-dependent manner. However, SM-exposed Raw264.7 cells were intact compared to U266 or U937 cells. Treatment with SM significantly elevated the generation of reactive oxygen species (ROS). The anti-proliferative effect of SM was reversed by pretreatment with the ROS scavenger, N-acetyl-l-cysteine (NAC), compared to cells treated only with SM. Also, SM treatment increased the ER stress by elevation of phosphorylated activating transcription factor 4 (p-ATF4), phosphorylated eukaryotic Initiation Factor 2 (p-eIF2), and phosphorylated protein kinase RNA-like endoplasmic reticulum kinase (p-PERK) expression. Caspase-3 and Poly (ADP-ribose) polymerase (PARP) were cleaved and CCAAT-enhancer-binding protein homologous protein (CHOP) was activated by SM treatment. PARP cleavage and CHOP activation were attenuated by NAC pretreatment. Furthermore, SM increased the tumor suppressor, miR-216b, and suppressed its target, c-Jun. miR-216b inhibitor attenuated the apoptotic effect of SM. Taken together, SM treatment induced apoptosis through regulation of miR-216b and ROS/ER stress pathways. SM could be a potential drug for treatment of multiple myeloma and myeloid leukemia.

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Section: Discussionmentioning

confidence: 99%

Activation of ER Stress-Dependent miR-216b Has a Critical Role in Salvia miltiorrhiza Ethanol-Extract-Induced Apoptosis in U266 and U937 Cells

Kim

Song

Park

et al. 2018

IJMS

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“…Chromatographic fingerprints of TCM are characterized by their integrity and ambiguity, and there are many evaluation indices in the fingerprint method which are greatly varied from HPLC quantification . For experimental design, the effect of multiple analytical parameters should be considered, such as the total number of peaks, the peak capacity factor, and peak purity, which are called the critical method attributes (CMAs) in the establishment of a HPLC fingerprint.…”

Section: Introductionmentioning

confidence: 99%

Establishing the chromatographic fingerprint of traditional Chinese medicine standard decoction based on quality by design approach: A case study of Licorice

Zhang

Wang

Chen

et al. 2019

J of Separation Science

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A novel analytical quality by design approach for developing a chromatographic fingerprint was established for analyzing complex traditional Chinese medicine, using a licorice standard decoction as an example. Considering the characteristics of integrity and ambiguity, the resolution of eight common peaks, total peak number, capacity factor distributions, and peak purity were selected as potential critical method attributes for assessing the quality of the chromatographic fingerprint. A central composite design was used to evaluate the relationship between critical method attributes and critical method parameters, including column temperature, wavelength, flow rate, formic‐acid concentration, and gradient parameters. A standard probability method was employed to calculate the design space of the fingerprint analysis parameters and evaluate the robustness of the methodology. The optimized high‐performance liquid chromatography fingerprint conditions were acetonitrile and 0.1% formic acid water gradient elution (0‐5 min, 5–19% A; 5–10 min, 19% A; 10–50 min, 19–42% A; 50–54 min, 42–100% A; 54–60 min, 100% A), column temperature 25±5°C, detection wavelength 265 nm. The design space of fingerprint analytical method based on the analytical quality by design approach not only met the requirements of the fingerprint analysis, but also improved the robustness and applicability of the fingerprint method.

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“…The construction and evaluation of chromatographic fingerprint is an important strategy in the quality control of traditional Chinese medicine because it can provide abundant information about complex chemical constituents of both markers and unknown components (Xue, Zhu, & Yi, ). Li et al () applied a fingerprint analysis method to 35 chromatographic peaks to evaluate the quality of Salvia miltiorrhiza Bunge. and identified 10 peaks with reference substances.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of polyphenols and triterpenes in pomegranate peel based on high‐performance liquid chromatography fingerprint by solvent extraction and ratio blending method in tandem with wavelength switching

Ding

Wang

Zhou

et al. 2019

Biomedical Chromatography

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Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. Pomegranate peel, the dried husk of P· granatum, is an important herbal medicine for treating diarrhea, hemostasis and insect-induced abdominal pain in China. However, the quality control methods for pomegranate peel remain unsatisfactory. In this work, a new HPLC-based qualitative and quantitative method for quality control of pomegranate peel was developed and validated for the simultaneous determination of polyphenols and triterpenes (including punicalagins A and B, ellagic acid, oleanolic acid and ursolic acid) by solvent extraction and ratio blending method in tandem with wavelength switching. The average recoveries were 98.07-100.61% with relative standard deviation no more than 4.27%. In addition, the fingerprint analysis was conducted to interpret the consistency of the quality test. Thirteen characteristic peaks were selected to evaluate the similarities of 16 batches of pomegranate peel. The similarities of samples were all more than 0.80, indicating that the samples from different areas of China were consistent. The results demonstrated that quantitative analysis and the HPLC fingerprint as a characteristic distinguishing method combining similarity evaluation can be successfully used to assess the quality and to identify the authenticity of pomegranate peel.

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Comparison of the chromatographic fingerprint, multicomponent quantitation and antioxidant activity of Salvia miltiorrhiza Bge. between sweating and nonsweating

Cited by 19 publications

References 52 publications

Activation of ER Stress-Dependent miR-216b Has a Critical Role in Salvia miltiorrhiza Ethanol-Extract-Induced Apoptosis in U266 and U937 Cells

Activation of ER Stress-Dependent miR-216b Has a Critical Role in Salvia miltiorrhiza Ethanol-Extract-Induced Apoptosis in U266 and U937 Cells

Establishing the chromatographic fingerprint of traditional Chinese medicine standard decoction based on quality by design approach: A case study of Licorice

Simultaneous determination of polyphenols and triterpenes in pomegranate peel based on high‐performance liquid chromatography fingerprint by solvent extraction and ratio blending method in tandem with wavelength switching

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