Comparison of the Bulk and Rhizosphere Soil Prokaryotic Communities Between Wild and Reintroduced Manglietiastrum sinicum Plants, a Threatened Species with Extremely Small Populations

Su, Daifa; Shen, Qingqing; Yang, Junyu; Li, Zhiying; Xiao, Wei; Wang, Yongxia; Ding, Zhuang; Cui, Xiao‐Long

doi:10.1007/s00284-021-02653-z

Cited by 3 publications

(7 citation statements)

References 48 publications

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“…This suggests that the effects of soil physicochemical properties on bacterial and fungal communities in the rhizosphere of P. squamata are different. Previous findings revealed that, among the nine soil physicochemical parameters, soil pH, TN, TP, and TK significantly affected the M. sinicum plant community at the genus level [39]. Our findings are similar to these results.…”

Section: Relationships Between Soil Physicochemical Properties and Ba...supporting

confidence: 92%

“…The relative abundance of Bacillus was lower in wild P. squamata individuals than in that of ex situ or reintroduced individuals at Qiaojia. The abundances of PGPR including Pseudomonas, Bacillus, Caulobacter, Flavobacterium, and Streptomyces in the reintroduced soils were lower than those in the wild soils; the decreased abundances of PGPR may be a biotic factor contributing to the endangerment of M. sinicum plants [39]. Our results are consistent with these findings.…”

Section: Structure Of the Rhizosphere Bacterial Community Of P Squamatasupporting

confidence: 90%

“…A total of 12 bacterial phyla were annotated in the rhizosphere of P. dabeshanensis, of which the dominant phyla were Proteobacteria, Acidobacteriota, Actinobacteriota and Chloroflexi [40]. Concerning bulk and rhizosphere soil prokaryotic communities between wild and reintroduced Manglietiastrum sinicum plants, the results presented Proteobacteria and Acidobacteria as the most abundant phyla [39]. Our results agree with these findings.…”

Section: Structure Of the Rhizosphere Bacterial Community Of P Squamatasupporting

confidence: 87%

“…Our results are similar to those from the P. squamata study at different conservation sites. Su et al (2021) compared the bulk and rhizosphere soil prokaryotic communities between wild and reintroduced Manglietiastrum sinicum plants; the alpha diversity differed slightly among WR (wild rhizosphere soils), WNR (wild bulk soils), RR (reintroduced rhizosphere soils), and RNR (reintroduced bulk soils), but the differences were not significant among sample groups [39]. Our results differ from those of the M. sinicum study in the richness and diversity in wild and reintroduced plant individuals.…”

Section: Diversity Of P Squamata Rhizosphere Soil Bacteriacontrasting

confidence: 86%

“…At the Qiaojia site, the ex situ (EQ) samples had a higher relative abundance of Actinobacteria than soils from the wild (WQ) and reintroduced (RQ) individuals. A previous study found that the relative abundance of Actinobacteria was higher in the rhizosphere surrounding wild plants than in reintroduced individuals in M. sinicum [39]. The endangered status of both M. sinicum and P. squamata may be attributed to the low abundance of Actinobacteria in the rhizosphere surrounding these reintroduced plants.…”

Section: Structure Of the Rhizosphere Bacterial Community Of P Squamatamentioning

confidence: 86%

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Comparison of Rhizosphere Bacterial Communities of Pinus squamata, a Plant Species with Extremely Small Populations (PSESP) in Different Conservation Sites

Li,

Lu,

Sun

2024

Microorganisms

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Pinus squamata is one of the most threatened conifer species in the world. It is endemic to northeastern Yunnan Province, China, and has been prioritized as a Plant Species with Extremely Small Populations (PSESP). The integrated study of soil properties and rhizosphere bacteria can assist conservation to understand the required conditions for the protection and survival of rare and endangered species. However, differences between the rhizospheric bacterial communities found in the soil surrounding P. squamata at different conservation sites remain unclear. In this study, Samples were collected from wild, ex situ, and reintroduced sites. Bacterial communities in different conservation sites of P. squamata rhizosphere soils were compared using Illumina sequencing. The soil physicochemical properties were determined, the relationships between the bacterial communities and soil physicochemical factors were analyzed, and the potential bacterial ecological functions were predicted. The reintroduced site Qiaojia (RQ) had the highest richness and diversity of bacterial community. Actinobacteria, Proteobacteria, and Acidobacteriota were the dominant phyla, and Bradyrhizobium, Mycobacterium, Acidothermus were the most abundant genera. Samples were scattered (R = 0.93, p = 0.001), indicating significant difference between the different conservation sites. The abundance of Mycobacterium differed between sites (0.01 < p ≤ 0.05), and the relative abundances of Bradyrhizobium and Acidothermus differed significantly among different sites (0.001 < p ≤ 0.01). Soil total potassium (TK) and available nitrogen (AN) were the main factors driving bacterial community at the phylum level (0.01 < p ≤ 0.05). This study generated the first insights into the diversity, compositions, and potential functions of bacterial communities associated with the rhizosphere soils of P. squamata in different conservation sites and provides a foundation to assess the effect of conservation based on bacterial diversity and plant growth-promoting rhizobacteria (PGPR) to guide future research into the conservation of P. squamata.

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Section: Relationships Between Soil Physicochemical Properties and Ba...supporting

confidence: 92%

Section: Structure Of the Rhizosphere Bacterial Community Of P Squamatasupporting

confidence: 90%

Section: Structure Of the Rhizosphere Bacterial Community Of P Squamatasupporting

confidence: 87%

Section: Diversity Of P Squamata Rhizosphere Soil Bacteriacontrasting

confidence: 86%

Section: Structure Of the Rhizosphere Bacterial Community Of P Squamatamentioning

confidence: 86%

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Comparison of Rhizosphere Bacterial Communities of Pinus squamata, a Plant Species with Extremely Small Populations (PSESP) in Different Conservation Sites

Li,

Lu,

Sun

2024

Microorganisms

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Evaluating the Endangered Mechanism and Different Conservation Effects of <em>Pinus squamata</em> from the Rhizosphere Soil Bacterial Community Perspective

Li,

Lu,

Sun

2024

Preprint

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Pinus squamata is one of the most threatened conifer endemic to northeastern Yunnan Province, China, and it is prioritized as one of the Plant Species with Extremely Small Populations (PSESP) for urgent protection in China. However, we lack a comprehensive consideration of the protection effect and the endangered mechanism, especially based on the underground growth status of endangered plants. The integrated study of soil properties and rhizosphere bacteria can assist conservation to understand therequired conditions for the protection and survival of rare and endangered species. In our study, bacterial communities in wild, ex-situ, and reintroduced P. squamata rhizosphere were compared using Illumina sequencing of the V3-V4 region of the 16S rRNA genes. We determined the soil physicochemical properties, analyzed the relationships between the bacterial communities and soil physicochemical factors, and predicted the potential bacterial ecological functions. The reintroduced site Qiaojia (RQ) had the highest richness and diversity. Samples were scattered (R = 0.93, P = 0.001), indicating significant difference between the different conservation sites. Soil total potassium (TK) and available nitrogen (AN) were the main factors driving bacterial community (0.01 &lt; P ≤ 0.05). The low abundance of Plant growth-promoting rhizobacteria (PGPR) may be a biotic factor contributing to the endangerment of P. squamata. This study provides a foundation to assess the effect of conservation based on bacterial diversity and plant growth-promoting rhizobacteria (PGPR) to guide future research into the conservation of P. squamata.

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Comparative analysis of the microbiomes of strawberry wild species Fragaria nilgerrensis and cultivated variety Akihime using amplicon-based next-generation sequencing

Wang,

Dai,

et al. 2024

Front. Microbiol.

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Fragaria nilgerrensis is a wild strawberry species widely distributed in southwest China and has strong ecological adaptability. Akihime (F. × ananassa Duch. cv. Akihime) is one of the main cultivated strawberry varieties in China and is prone to infection with a variety of diseases. In this study, high-throughput sequencing was used to analyze and compare the soil and root microbiomes of F. nilgerrensis and Akihime. Results indicate that the wild species F. nilgerrensis showed higher microbial diversity in nonrhizosphere soil and rhizosphere soil and possessed a more complex microbial network structure compared with the cultivated variety Akihime. Genera such as Bradyrhizobium and Anaeromyxobacter, which are associated with nitrogen fixation and ammonification, and Conexibacter, which is associated with ecological toxicity resistance, exhibited higher relative abundances in the rhizosphere and nonrhizosphere soil samples of F. nilgerrensis compared with those of Akihime. Meanwhile, the ammonia-oxidizing archaea Candidatus Nitrososphaera and Candidatus Nitrocosmicus showed the opposite tendencies. We also found that the relative abundances of potential pathogenic genera and biocontrol bacteria in the Akihime samples were higher than those in the F. nilgerrensis samples. The relative abundances of Blastococcus, Nocardioides, Solirubrobacter, and Gemmatimonas, which are related to pesticide degradation, and genus Variovorax, which is associated with root growth regulation, were also significantly higher in the Akihime samples than in the F. nilgerrensis samples. Moreover, the root endophytic microbiomes of both strawberry species, especially the wild F. nilgerrensis, were mainly composed of potential biocontrol and beneficial bacteria, making them important sources for the isolation of these bacteria. This study is the first to compare the differences in nonrhizosphere and rhizosphere soils and root endogenous microorganisms between wild and cultivated strawberries. The findings have great value for the research of microbiomes, disease control, and germplasm innovation of strawberry.

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Comparison of the Bulk and Rhizosphere Soil Prokaryotic Communities Between Wild and Reintroduced Manglietiastrum sinicum Plants, a Threatened Species with Extremely Small Populations

Cited by 3 publications

References 48 publications

Comparison of Rhizosphere Bacterial Communities of Pinus squamata, a Plant Species with Extremely Small Populations (PSESP) in Different Conservation Sites

Comparison of Rhizosphere Bacterial Communities of Pinus squamata, a Plant Species with Extremely Small Populations (PSESP) in Different Conservation Sites

Evaluating the Endangered Mechanism and Different Conservation Effects of <em>Pinus squamata</em> from the Rhizosphere Soil Bacterial Community Perspective

Comparative analysis of the microbiomes of strawberry wild species Fragaria nilgerrensis and cultivated variety Akihime using amplicon-based next-generation sequencing

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