Comparison of the bone healing capacity of autogenous bone, demineralized freeze dried bone allograft, and collagen sponge in repairing rabbit cranial defects

Abstract

(J Korean Assoc Oral Maxillofac Surg 2012;38:221-30) Objectives: This study sought to evaluate the efficacy of collagen graft materials, as compared to other graft materials, for use in healing calvarial defects in rabbits. Materials and Methods: Ten mm diameter calvarial defects were made in ten rabbits. The rabbits were then divided into 4 groups: control, autogenous bone graft, SureOss graft, and Teruplug graft. Bone regeneration was evaluated using histological and radiographic methods. Results: Based on visual examination, no distinct healing profile was observed. At 4 weeks after treatment, histological analysis showed there was no bone regeneration in the control group; however, at 8 weeks after treatment, new bone formation was observed around the margin of the defective sites. In the autogenous bone graft group, new bone formation was observed at 4 weeks after treatment and mature bone was detected around the grafted bone after 8 weeks. In the SureOss graft group, at 4 weeks after treatment, acute inflammatory and multinuclear cells were noted around the grafted materials; at 8 weeks after treatment, a decrease in graft materials coupled with new bone formation were observed at the defective sites. In the Teruplug graft group, new bone formation was detected surrounding the bone margin and without signs of inflammation. There were statistically significant differences observed between the graft and control group in terms of bone density as evidenced by radiographic analysis using computed tomography (P<0.05), particularly for the autogenous bone graft group (P<0.001). Conclusion: These results suggested that autogenous bone, SureOss and Teruplug have the ability to induce bone regeneration as compared to an untreated control group. The osteogenic potential of Teruplug was observed to be lower than that of autogenous bone, but similar to that of SureOss.

Using Raman spectroscopy to estimate the demineralization of bone transplants during preparation

“…1 However, bone tissue obtained from the donor's bones has the advantage that no additional surgical intervention is needed in the patient's body, and the biological tissue can be used immediately after the implantation biomaterial is removed. 2 Moreover, a donor's bone tissue used in transplantology, being foreign with respect to the recipient's bone tissue, can cause certain side effects, such as immune rejection and infection. 3 A donor's demineralized lyophilized bone tissue is a possible alternative bone transplant that is virtually just as good as one based on the patient's bone tissue.…”

“…makes it possible to record the mineral composition of bone tissue; however, it is not able to use the characteristics to distinguish the forms of the minerals that constitute the crystal-lattice structure of the mineral. 6 Hur et al 2 used computer tomography to estimate the degree of formation of the bone tissue of rabbits after autogenous and untreated bone samples were incorporated, from the viewpoint of the density of the bone tissue. As a result, severe inflammatory effects were observed in a group of samples four weeks after untreated bone material was incorporated, at the same time as the formation of new bone mass with no symptoms of inflammation was observed in the group in which autogenous bone tissues were incorporated.…”

Evaluation of Acellular Bovine Cancellous Bone Matrix Seeded with Foetal Rabbit Osteoblasts for Segmental Bone Defects in Rabbits

“…Early calcification of grafted bone due to transformation of osteoblasts into osteocytes and liberation of Ca 2+ ion in the matrix was bringing about formation of bony union in control group and group C (Breitbart et al, 2010). Neovascularisation was seen near the side of the graft and not at the edge of the graft, due to integration of graft to the host bone (Hur et al, 2012). At host-graft junction intense osteoblastic activity and disintegrated scaffold were found in group C (Fig 4 C 90).…”

Tearable and Fillable Composite Sponges Capable of Heat Generation and Drug Release in Response to Alternating Magnetic Field

“…Currently, collagen sponges are used in clinical practice due to their handleability. For example, Teruplug ® is filled into tooth extraction wounds for hemostasis, the protection of the wound surface, and the promotion of tissue formation [50]. This fact suggests that MDC sponges, which have favorable handleability, are considered applicable in clinical practice.…”