Comparison of the Biodegradation of n-alkanes and Readily Biodegradable Substrates Using Open Mixed Culture under Aerobic, Anoxic and Anaerobic Conditions

Abstract

This study has investigated the biodegradation of n-alkanes using open mixed cultures in batch tests.Biodegradation of n-C12, C14, C16, C18, C20 was investigated using a respirometric method and compared with the biodegradation of the readily biodegradable substrates glucose, acetic acid and ethanol. Experiments were performed in small-scale bioreactors under various conditions, i.e. aerobic, anoxic with nitrate and completely anaerobic conditions, using two different sources of open mixed microbial cultures. Under aerobic conditions all the readily biodegradable substrates and hydrocarbons were removed, although the acclimation time was longer for the hydrocarbons (3-5 days) than for the readily biodegradable substrates (1-2 days). No significant effect on the hydrocarbon concentration on biodegradation was observed in the concentration range 0.5-5 g/l. Under anoxic conditions, both the readily biodegradable substrates and the hydrocarbons were removed using nitrate as electron acceptor. However, the acclimation time for the hydrocarbons under anoxic conditions (20-25 days) was much longer than under aerobic conditions. Under both aerobic and anoxic conditions, once acclimation with the hydrocarbons was completed, the microorganisms were immediately able to remove a second spike of the substrate without acclimation. Under anaerobic conditions, no activity was observed with the hydrocarbons over a period of 150 days, while the mixed culture was able to remove glucose and convert it to volatile fatty acids. Under aerobic conditions, the dissolved oxygen consumption data was mathematically modelled using Monod kinetics to obtain biokinetic parameters. Good fittings between the model and the experimental data was obtained and the biodegradation of hydrocarbons was characterised by higher values of the parameter K S compared to the readily biodegradable substrates. The results of this study showed that the open mixed microbial cultures contained diverse microorganisms capable of utilizing both liquid and solid n-alkanes under aerobic and anoxic conditions.

Effect of process conditions on the aerobic biodegradation of phenol and paracetamol by open mixed microbial cultures

“…FromFigure 1awe calculated the acclimation time, defined as the time required for the microorganisms to become able to degrade phenol and measured as the time required for the oxygen concentration to drop by at least 1 mg/l. This method of measuring acclimation time, although it is arbitrary, is consistent with our previous studies[14] and allows a consistent comparison between experiments done with the same methodology.Figure 1bshows the acclimation time vs the initial phenol concentration. This plot indicates that the acclimation time was longer for higher phenol concentrations.…”

Model‐centric optimisation of biochemical remediation of petroleum hydrocarbon contaminated soil

“…The premise is that more soluble hydrocarbons move from polluted soils to water, leading to additional transport and bioremediation (Fernández et al., 2016; Geng et al., 2014; Nicol et al., 1994). Most models formulated for soils are suitable for ex‐situ bioremediation (Ebenhöh & Berthe‐Corti, 2001; Kosterin & Sofinskaya, 2010; Uzukwu & Dionisi, 2017). Further, several works emphasized mathematical approaches that include mass transfer with Monod or first‐order kinetics, concerning hydrocarbon breakdown, specifically those with relatively less solubility (Park et al., 2001; Woo et al., 2001).…”

A Mathematical Model for Bioremediation of Hydrocarbon-Contaminated Soils

“…As such, sequestration of this less soluble fraction of oil may occur in soils; its environmental fate is still not sufficiently studied. In soils, the majority of the developed models are designed for ex situ bioremediation [14,[18][19][20]. In addition, only a few mathematical models couple mass transfer with Monod or first-order kinetics for hydrocarbon biodegradation [16,19,[21][22][23], especially for less soluble hydrocarbons [16].…”