Comparison of the API 20E and BBL Crystal E/NF Identification Systems for Differentiating Bacterial Isolates from Apparently Healthy Reared Sea Bass (Dicentrarchus labrax)

Popović, Natalija Topić; Skukan, Andrea Benussi; Strunjak‐Perović, Ivanc̆ica; Čož‐Rakovac, Rozelindra; Hacmanjek, Mato; Hunjak, Blaženka

doi:10.1023/b:verc.0000012113.95479.2f

Cited by 7 publications

(6 citation statements)

References 6 publications

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“…Randomly taken samples of bacteria and yeasts were characterized with API 20E and API 20 AUX strips, respectively (BioMérieux, Marcy l'Etoile, France). API 20E is one of the most commonly used kits for the rapid diagnosis of bacteria from fish (Popovic et al ., 2004). The strips were used according to the manufacturer's instructions, except for the suspension medium, which was replaced by sterile half‐salinity water for the marine isolates.…”

Section: Methodsmentioning

confidence: 99%

Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)

Silva

Nicoli

Zambonino-Infante

et al. 2011

FEMS Microbiology Ecology

105

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Fish intestinal microbiota changes with the diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. The objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (Sparus aurata) and in goldfish (Carassius auratus). Faecal, gastrointestinal and intestinal contents were characterized using culture-based and molecular methods. Vibrionaceae was high in faeces and in the intestine of sea bream, while a more diverse microbiota was retrieved from the stomach, where Bacillales and Flavobacteriaceae appeared to be influenced by the diet. PCR-denaturing gradient gel electrophoresis profiles revealed a high diversity of the microbiota transiting in the sea bream digestive tract, with a shift between gastric and intestinal communities, especially in the group fed with lupin meal. The goldfish was different, with a predominance of Aeromonas spp., Shewanella putrefaciens and Staphylococcus spp. among the aerotolerant-cultivable bacteria. The culture-independent methods revealed the presence of anaerobes like Cetobacterium somerae, and that of Vibrio spp., likely in a viable, but noncultivable state. There was a trend towards decreasing diversity in goldfish microbiota with the partial substitution by lupin, which seemed to inhibit some taxa.

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Section: Methodsmentioning

confidence: 99%

Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)

Silva

Nicoli

Zambonino-Infante

et al. 2011

FEMS Microbiology Ecology

105

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“…The phenotypic characterisation of the isolates was determined with API 20 E strips according to manufacturer's instructions (bioMérieux). It has been reported that API 20E is the most commonly used kit for rapid diagnosis of bacteria from fish (Popovic et al, 2004). API 20 E profiles were compared using the Wagner parsimony method, using Mix of Phylip (Felsenstein, 1996), to arrange the strains in dendrograms drawn with Fixdtree (Felsenstein, 1996) in order of similarity.…”

Section: Microbiological Methodsmentioning

confidence: 99%

Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae

Kotzamanis

Gisbert

Gatesoupe

et al. 2007

Comparative Biochemistry and Physiology Part A: Molecular &

212

151

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Two fish protein hydrolysates (FPH) were incorporated into four diets prepared for start-feeding sea bass larvae, at two different levels (10% and 19% of total ingredients): a commercial FPH, CPSP, in which the molecular mass of the main fraction of soluble peptides (51%) was between 500-2500 Da, and an experimental FPH obtained by acidic silage of sardine offal, SH, with a main portion of soluble peptides (54%) ranging from 200 to 500 Da. The diet with 10% of the commercial FPH gave the best results in terms of growth, survival and intestinal development, as evaluated by the early activity of digestive enzymes in the brush border membrane (alkaline phosphatase and aminopeptidase N). This was related to the low level of Vibrio spp. counted in the larvae of group C10. The high dose of FPH, especially in the experimental preparation rich in short peptides, seemed to favour the dominance of Vibrio sp. TYH3, which behaved opportunistically. The effect of the experimental FPH was ambiguous, since early larvae challenged with Vibrio anguillarum were more resistant to the pathogen, especially at high FPH dose (group S19). This might be due either to direct antagonism between V. anguillarum and Vibrio sp. TYH3, or to the stimulation of the immune response in the larvae. These results indicate that different molecular weight fractions and concentrations of feed-soluble peptides may affect the growth performance and immunological status of sea bass larvae. Consequently, a low dose of commercial FPH seems advisable, both for larval development and for the bacterial environment, although further research is required to determine and characterize peptide fractions that may have a beneficial effect on growth and immune response, and to determine their optimal inclusion levels in diets for sea bass larvae.

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“…For the identification of sea fish isolates, Grisez et al (1991) suggested the following modifications of the prescribed method for the inoculation of the test strip: increasing of the incubation time to 48-72 hours, lowering the incubation temperature to 26°C, using a suspension of 1.5% saline as inoculum, and allowing only fermentation of sugars by sealing these cups with sterile mineral oil. In our previous study we described sea fish bacteria determination according to Grisez et al (1991) additionally lowering the incubation temperature to 22°C (Coz-Rakovac et al, 2002;Topic Popovic et al, 2004). Bertone et al (1996) used modified API 20E for sea fish bacteria, particularly a 2% NaCl modified diluent, and incubation at 25°C for 24-72 hours, according to MacDonnel et al (1982).…”

Section: Adaptation Of Api 20e For Fish Bacterial Isolatesmentioning

confidence: 99%

Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

Popović¹,

Čož‐Rakovac²,

Strunjak‐Perović³

2007

Vet. Med.

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ABSTRACT:The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as Yersinia ruckeri, Edwardsiella ictaluri, Vibrio anguillarum.

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Comparison of the API 20E and BBL Crystal E/NF Identification Systems for Differentiating Bacterial Isolates from Apparently Healthy Reared Sea Bass (Dicentrarchus labrax)

Cited by 7 publications

References 6 publications

Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)

Influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (Sparus aurata) and intestinal contents in goldfish (Carassius auratus)

Effects of different dietary levels of fish protein hydrolysates on growth, digestive enzymes, gut microbiota, and resistance to Vibrio anguillarum in European sea bass (Dicentrarchus labrax) larvae

Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

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