Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission

Alexandre, Kabamba B.; Malatji, Kanyane; Mulaudzi, Takalani

doi:10.1016/j.virol.2020.01.017

Cited by 7 publications

(6 citation statements)

References 54 publications

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“…This agrees with prior findings in vitro and in vivo in mice with cyanovirin (Smee et al, 2008). Griffithsin has been shown to inhibit a number of enveloped viruses, but to our knowledge, this is the first demonstration of antiviral activity for IAV (Alexandre et al, 2020). The activity of griffithsin was somewhat less than that of cyanovirin, in general.…”

Section: Discussionsupporting

confidence: 90%

Viral Evasion of Innate Immune Defense: The Case of Resistance of Pandemic H1N1 Influenza A Virus to Human Mannose-Binding Proteins

et al. 2021

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Mannose-binding lectins effectively inhibit most seasonal strains of influenza A virus and contribute to the innate host defense vs. these viruses. In contrast, pandemic IAV strains are largely resistant to these lectins, likely contributing to increased spread and worse outcomes. In this paper, we evaluated the inhibition of IAV by mannose-binding lectins of human, bacterial, and fungal origin to understand and possibly increase activity vs. the pandemic IAV. A modified version of the human surfactant protein D (SP-D) neck and carbohydrate recognition domain (NCRD) with combinatorial substitutions at the 325 and 343 positions, previously shown to inhibit pandemic H3N2 IAV in vitro and in vivo, and to inhibit pandemic H1N1 in vitro, failed to protect mice from pandemic H1N1 in vivo in the current study. We attempted a variety of maneuvers to improve the activity of the mutant NCRDs vs. the 2009 pandemic H1N1, including the formation of full-length SP-D molecules containing the mutant NCRD, cross-linking of NCRDs through the use of antibodies, combining SP-D or NCRDs with alpha-2-macroglobulin, and introducing an additional mutation to the double mutant NCRD. None of these substantially increased the antiviral activity for the pandemic H1N1. We also tested the activity of bacterial and algal mannose-binding lectins, cyanovirin, and griffithsin, against IAV. These had strong activity against seasonal IAV, which was largely retained against pandemic H1N1. We propose mechanisms to account for differences in activity of SP-D constructs against pandemic H3N2 and H1N1, and for differences in activity of cyanovirin vs. SP-D constructs.

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Section: Discussionsupporting

confidence: 90%

Viral Evasion of Innate Immune Defense: The Case of Resistance of Pandemic H1N1 Influenza A Virus to Human Mannose-Binding Proteins

et al. 2021

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“… Lectin (Griffithsin) In vivo, antiviral Mannose-binding lectin binds with viral envelope GPs, inhibiting viral infection of HIV-1, HSV, and HPV [ 75 ] In vitro, antiviral Mannose-binding lectin binds with Spike GPs, inhibiting viral infection of SARS-CoV [ 82 ] In vitro, antiviral Griffithsin and synthetic polymers showed high virucidal activity in cell-associated HIV-1 and in the presence of seminal and vaginal simulants. Blocking of CD4+ viral binding [ 83 ] Grateloupia chiangii Lectin In vitro, antiviral Mannose-binding lectin binds with viral envelope GPs, inhibiting viral infection of Influenza H1N1 and HSV1/2 [ 79 ] Kappaphyrus alvarezii Lectin In vitro, antiviral Mannose-binding lectin binds to Influenza hemagglutinin and HIV GP gp120, avoiding infection [ 84 ] Kappaphycus striatum Lectin In vitro, antimicrobial Growth inhibition of Vibrio alginolyticus (25.4 μg/mL) and Enterococcus cloacae (101.6 μg/mL). Did not inhibit other Vibrio spp., Staphylococcus aureus , or Escherichia coli [ 85 ] Gracilaria fisheri Lectin In vivo, antimicrobial There was >50% mortality reduction in 100 μg/mL treated shrimp, related with agglutination of Vibrio parahaemolyticus …”

Section: Bioactive Properties and Mechanisms Of Actionmentioning

confidence: 99%

Seaweed-Derived Proteins and Peptides: Promising Marine Bioactives

et al. 2022

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Seaweeds are a typical food of East-Asian cuisine, to which are alleged several beneficial health effects have been attributed. Their availability and their nutritional and chemical composition have favored the increase in its consumption worldwide, as well as a focus of research due to their bioactive properties. In this regard, seaweed proteins are nutritionally valuable and comprise several specific enzymes, glycoproteins, cell wall-attached proteins, red algae phycobiliproteins, lectins, peptides, or mycosporine-like amino acids. This great extent of molecules has been reported to exert significant antioxidant, antimicrobial, anti-inflammatory, antihypertensive, antidiabetic, or antitumoral properties. Hence, knowledge on algae proteins and derived compounds have gained special interest for the potential nutraceutical, cosmetic or pharmaceutical industries based on these bioactivities. Although several molecular mechanisms of action on how these proteins and peptides exert biological activities have been described, many gaps in knowledge still need to be filled. Updating the current knowledge related to seaweed proteins and peptides is of interest to further asses their potential health benefits. This review addresses the characteristics of seaweed protein and protein-derived molecules, their natural occurrence, their studied bioactive properties, and their described potential mechanisms of action.

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“…Griffithsin well-known anti-viral lectin, was expressed in Lactobacillus rhamnosus GR-1 probiotic strains for vaginal mucosal delivery of lectin to inhibit HIV transmission and subsequent replication [ 27 ]. Another research group has used Nicotiana benthamiana as the expression to over express Griffithsin lectin (as an HIV-1 entry inhibitor) by using three gene silencing suppressors, with a yield of 400 μg/g fresh weight after expression which was reduced to 287 μg/g after purification with a total recovery of 71.75% [ 28 ]. Later on, the monomeric Griffithsin lectin was linked in tandem repeats of two (2MG and 2MG3), three (3MG) and four (4MG) units and found that 2MG and 2MG3 tandemers had similar activity to Griffithsin against cell-free and cell-associated HIV-viruses, while 3MG and 4MG were significantly more potent than monomeric Griffithsin against HIV-viruses [ 29 ].…”

Section: Algal Lectins Against Antivirus Propertiesmentioning

confidence: 99%

Lectins from plants and algae act as anti-viral against HIV, influenza and coronaviruses

Naik

Kumar

2022

Mol Biol Rep

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Background Carbohydrate–lectin interactions are extremely specific as the lectin is capable of recognising monomeric and oligomeric sugars in a reversible manner. It has been known for a long time that lectins have antibacterial, antifungal, and insecticidal activities. Recently, it has been reported that many lectins can prevent the virus growth by interacting with the viral envelop surface glycoprotein. Spike protein, which is found on the surface of some enveloped viruses, is heavily mannosylated and will have strong affinity for mannose specific lectins. According to the findings, lectins have a high binding affinity for the glycans of the SARS-CoV-2 spike glycoprotein, which contains N-glycosylation sites. As a result, various lectins are being researched and developed as anti-viral agents. Results According to our in silico studies, the amino acid residues Asn487, Tyr489, Gln493, Lys417, and Tyr505 of the receptor binding domain (RBD) of SARS-CoV-2 formed an interaction with the model lectin Lablab purpureus lectin. Similar interaction for SARS-CoV-2 spike protein was observed with Griffithsin lectin (algal source) as well. These observations demonstrate that lectins could be one of the potential molecules for neutralising coronavirus infection. Conclusion This review focuses on anti-viral lectins isolated and characterized from plants and algae (last 5 years) and showed anti-viral properties against HIV, Influenza, and coronaviruses.

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Comparison of the antiviral activity of the microbicide candidate griffithsin and its tandemers derivatives against different modes of HIV-1 transmission

Cited by 7 publications

References 54 publications

Viral Evasion of Innate Immune Defense: The Case of Resistance of Pandemic H1N1 Influenza A Virus to Human Mannose-Binding Proteins

Viral Evasion of Innate Immune Defense: The Case of Resistance of Pandemic H1N1 Influenza A Virus to Human Mannose-Binding Proteins

Seaweed-Derived Proteins and Peptides: Promising Marine Bioactives

Lectins from plants and algae act as anti-viral against HIV, influenza and coronaviruses

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