Comparison of the Anticoagulant Action of Sulfated and Phosphorylated Polysaccharides

Dace, R; McBride, Ethan M.; Brooks, Katherine M.; Gander, J.E.; Buszko, Marian L.; Doctor, V. M.

doi:10.1016/s0049-3848(97)00110-2

Cited by 50 publications

(22 citation statements)

References 16 publications

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“…Fucoidan purchased commercially, was heterogeneous and was purified by ultra filtration and diethylaminoethyl cellulose (DEAE) chromatography followingthe procedure reported previously (12). Purified fucoidan was sulfated using chlorosulfonic acid-pyridine complex and isolated as the sodium salt by the procedure described earlier (18). The % sulfate in fucoidan was increased from 28% to 55% by the sulfation procedure (18).…”

Section: Methodsmentioning

confidence: 99%

“…Purified fucoidan was sulfated using chlorosulfonic acid-pyridine complex and isolated as the sodium salt by the procedure described earlier (18). The % sulfate in fucoidan was increased from 28% to 55% by the sulfation procedure (18). A model ELx 800 Well Counter (Bio-Tek Instruments, Winooski, Vermont, USA) was used at a wavelength of 405 nm for the rate study.…”

Section: Methodsmentioning

confidence: 99%

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Mechanism of the stimulatory effect of native fucoidan, highly sulfated fucoidan and heparin on plasminogen activation by tissue plasminogen activator: The role of chloride

Lang

Williams

Phillips

et al. 2004

European Journal of Drug Metabolism and Pharmacokinetics

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Native Fucoidan and unfractionated heparin enhanced by 6-fold the in vitro activation of human glutamic plasminogen (Glu-Plg) by tissue plasminogen activator (t-PA) using 0.05M Tris buffer pH 7.4, while sulfated fucoidan inhibited the activation under these conditions. Double reciprocal plots of these interactions showed that sulfated fucoidan inhibited the activation in a noncompetitive manner while the enhancements by heparin or native fucoidan were due to an increase of Vmax without affecting Km. To determine whether the stimulatory effect of the individual cofactor was due to its interaction with Glu-Plg or with t-PA, experiments were performed at a fixed level of the cofactor and either varying in a serial fashion the concentration of Glu-Plg or of t-PA. The ratios of the initial rate of plasmin generation in the presence or absence of the cofactors were plotted against the inverse of the volume fraction of Glu-Plg or of t-PA. The results showed that heparin interacted with Glu-Plg while native fucoidan and sulfated fucoidan interacted with t-PA. Studies were also conducted on the effect of the two fucoidans and heparin on the activation of Glu-Plg by t-PA using 0.05M Tris buffer pH 7.4 containing 0.1 M NaCl. Under these conditions, sulfated fucoidan was most effective in enhancing the activation followed by native fucoidan and heparin respectively. The results of this study showed that in presence of the buffer containing 0.1 M NaCl, heparin was interacting with t-PA while the two fucoidans were interacting with both t-PA and Glu-Plg. A comparison of the double reciprocal plots of the rate of enhancement by the cofactors using 0.05M Tris buffer pH 7.4 containing 0.1M NaCl or in presence of buffer alone showed that the cofactors were more effective using 0.05M Tris buffer pH 7.4 alone and that addition of NaCl to the buffer slowed down the reactions by decreasing Vmax without affecting Km. Circular Dichroism (CD) studies of Glu-Plg in the far ultraviolet (UV) range showed that addition of NaCl destabilized the beta sheet structure which was reversed by addition of 6-aminohexanoic acid (6-AH) or one of the cofactors, while the near UV CD spectra of Glu-Plg in presence of 0.1 M NaCl was enhanced by the cofactors by increasing its ellipticity as reported earlier for 6-AH.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Mechanism of the stimulatory effect of native fucoidan, highly sulfated fucoidan and heparin on plasminogen activation by tissue plasminogen activator: The role of chloride

Lang

Williams

Phillips

et al. 2004

European Journal of Drug Metabolism and Pharmacokinetics

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“…8,9 Another interesting sulfated polysaccharide is the sulfated fucan, which displays anticoagulant and antithrombotic activities. 1,10,11 Also known as fucoidan (FC), 12 this substance is found in brown algae (Fucus vesiculosus) and displays an ability to increase the inhibition of thrombin by antithrombin or heparin cofactor II. 1,2 Biopolymers such as CT are considered natural complexing agents, with the ability to form physical gels or coacervates with sulfated polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Silva¹,

Garcia²,

Mori³

et al. 2012

IJN

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Abstract:The aim of this study was to produce and characterize nanoparticles (NPs), combining chondroitin sulfate (CS) and fucoidan (FC) with chitosan for therapeutic purposes. These NPs were characterized by dynamic light scattering, zeta potential determination, and transmission electronic microscopy. The anticoagulant activity was determined for FC NPs and compared with FC solution at the same concentration. FC NPs showed regular shapes and better anticoagulant activity than free polysaccharide solution. FC solution did not affect coagulation compared to FC NPs, which increased up to two-fold, even at a lower concentration. Cytotoxicity and permeability tests were conducted using Caco-2 cell monolayer, exhibiting no toxic effect in this cell line and higher permeability for NP2 samples than FC solution at the same concentration.

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“…Heparin exhibited the antithrombin III (AT-III) in order to inhibit blood clotting factors, factor Xa, II and XII. (Keijer et al, 1991;Bombardini and Picano, 1997;Dace et al, 1997). Guava leaf extract may have similar coagulation effects to heparin.…”

Section: Discussionmentioning

confidence: 99%

Guava leaf extract and topical haemostasis

Jaiarj¹,

Wongkrajang²,

Thongpraditchote³

et al. 2000

Phytother. Res.

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Comparison of the Anticoagulant Action of Sulfated and Phosphorylated Polysaccharides

Cited by 50 publications

References 16 publications

Mechanism of the stimulatory effect of native fucoidan, highly sulfated fucoidan and heparin on plasminogen activation by tissue plasminogen activator: The role of chloride

Mechanism of the stimulatory effect of native fucoidan, highly sulfated fucoidan and heparin on plasminogen activation by tissue plasminogen activator: The role of chloride

Preparation and characterization of polysaccharide-based nanoparticles with anticoagulant activity

Guava leaf extract and topical haemostasis

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