Comparison of the Antibodies Elicited by the Individual Structural Polypeptides of Foot-and-Mouth Disease and Polio Viruses

Meloen, R. H.; Rowlands, David J.; Brown, F.

doi:10.1099/0022-1317-45-3-761

Cited by 85 publications

(44 citation statements)

References 1 publication

(1 reference statement)

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“…However, they failed to react with polypeptide VP4 or intact virus and also failed to neutralize poliovirus infectivity. Antibodies against polypeptide VP1 of foot-and-mouth disease virus (FMDV) prepared in a similar way did have neutralizing activity (Meloen et al, 1979). The observation that antisera against purified preparations of VP1 of FMDV types O and A had neutralizing activity was also reported earlier (Laporte et al, 1973;Bachrach et al, 1975;Kaaden et al, 1977).…”

Section: Introductionsupporting

confidence: 49%

“…This is understandable, because of the smaller amount of VP4 used for immunization (see Methods). Earlier attempts to obtain monospecific antisera against VP4 were unsuccessful (Meloen et al, 1979;Vrijsen et al, 1980). The observation that the monospecific antiserum against VP3 recognizes an antigenic site on the surface of native virions is striking.…”

Section: Discussionmentioning

confidence: 99%

“…The antisera obtained were monospecific for the polypeptides VP1, VP2 and VP3 when tested by radioimmunoassay (Meloen et al, 1979) or in an immunoprecipitation test (Vrijsen et al, 1980). However, they failed to react with polypeptide VP4 or intact virus and also failed to neutralize poliovirus infectivity.…”

Section: Introductionmentioning

confidence: 93%

“…Nevertheless, SDS-PAGE has been used for the preparation of individual poliovirus polypeptides for use as immunogens (Meloen et al, 1979;Vrijsen et al, 1980). The antisera obtained were monospecific for the polypeptides VP1, VP2 and VP3 when tested by radioimmunoassay (Meloen et al, 1979) or in an immunoprecipitation test (Vrijsen et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

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Monospecific Antisera Against Capsid Polypeptides of Poliovirus Type 1 Distinguish Antigenic Structures of Poliovirus Proteins

Wiegers

Dernick

1983

Journal of General Virology

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SUMMARYPure poliovirus polypeptides, namely VP1, VP2, VP3 and VP4, have been isolated by isoelectric focusing after dissociation of poliovirus by urea. When injected into rabbits, all four polypeptides produced monospecific antisera which were used for the characterization of poliovirus particles and poliovirus-infected cells. The specificity of these antisera was determined by immunoprecipitation of polypeptides obtained by dissociation of poliovirus with SDS, followed by characterization by polyacrylamide gel electrophoresis. The antisera revealed differences in the antigenic sites of native poliovirus particles, heated poliovirus particles and naturally occurring empty capsids. Only VP3 antiserum reacted with native poliovirus and showed some neutralization, whereas all antisera precipitated heated virus and empty capsids. These antisera reacted also with the appropriate precursors of the capsid polypeptides demonstrating their usefulness for an analysis of the cleavage pathway by monospecific antibodies and revealed a second protomer (90 kilodalton) polypeptide for the capsid proteins of poliovirus particles.

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Section: Introductionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Monospecific Antisera Against Capsid Polypeptides of Poliovirus Type 1 Distinguish Antigenic Structures of Poliovirus Proteins

Wiegers

Dernick

1983

Journal of General Virology

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“…This is in marked contrast to the response of unprimed guinea-pigs to I2S particles, when the level of neutralizing antibody produced is much less than that produced by 146S particles (Brown & Crick, 1959;Ceglowski, I965). The studies of Meloen et al (1979) showed that I46S particles elicited a greater neutralizing antibody response than IO times the amount of I2S particles.…”

Section: Discussionmentioning

confidence: 99%

Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus

Cartwright

Chapman

Brown

1980

Journal of General Virology

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SUMMARYIntact 146S particles of the seven serotypes of foot-and-mouth disease virus (FMDV) produce type-specific precipitating, complement-fixing and neutralizing antibodies in cattle and guinea-pigs. However, the 12S structural subunit, produced from the virus particle by mild acid treatment (pH 6) or by heating at 56 °C, although stimulating the production of precipitating and complement-fixing antibodies, produces only low levels of neutralizing antibody. Nevertheless, IzS particles were as active as 146S particles in stimulating the production of neutralizing antibody in guinea-pigs primed with a vaccine prepared from L46S particles. Moreover, heterotypic ~zS and I46S particles also boosted the neutralizing antibody response to the first virus. These results point to an antigenic similarity between the I46S particles of each type and to a close antigenic relationship between the r46S and IzS particles.

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Influence of Local Structure on the Location of Antigenic Determinants in Tobacco Mosaic Virus Protein

Regenmortel

Altschuh

Klug

2007

Novartis Foundation Symposia

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Comparison of the Antibodies Elicited by the Individual Structural Polypeptides of Foot-and-Mouth Disease and Polio Viruses

Cited by 85 publications

References 1 publication

Monospecific Antisera Against Capsid Polypeptides of Poliovirus Type 1 Distinguish Antigenic Structures of Poliovirus Proteins

Monospecific Antisera Against Capsid Polypeptides of Poliovirus Type 1 Distinguish Antigenic Structures of Poliovirus Proteins

Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus

Influence of Local Structure on the Location of Antigenic Determinants in Tobacco Mosaic Virus Protein

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