Comparison of the antibacterial effect of silver sulfadiazine 1%, mupirocin 2%, Acticoat and octenidine dihydrochloride in a full-thickness rat burn model contaminated with multi drug resistant Acinetobacter baumannii

Selçuk, Caferi Tayyar; Durğun, Mustafa; Özalp, Burhan; Tekin, Alicem; Tekin, Recep; Akçay, Cemal; Alabalık, Ulaş

doi:10.1016/j.burns.2012.04.009

Cited by 21 publications

(14 citation statements)

References 27 publications

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“…Despite the antibacterial and wound healing properties, ASHE and ASDE ointment formulations applied to mice burn wounds formed a pseudoeschar on the wound surface promoting its bioavailability. This pseudoeschar cohering intrusively to mice tissues could be one of the main reasons for ASHE/ASDE to remain on burn wounds [23]. No such pseudoeschar is formed in mice groups treated with SOB or SSD ointment.…”

Section: Discussionmentioning

confidence: 99%

“…Thermal injury procedure was introduced according to the method described previously [23]. Briefly, the dorsal surface of the mice was shaved (2 × 3 cm) one day prior to the experiment.…”

Section: Methodsmentioning

confidence: 99%

“…The progressive healing of the wound size was evaluated by tracing the wound after every 5-day interval (for 20 consecutive days) using transparent paper and a pencil, and the recorded wound area was measured graphically [23]. Wound contraction rates were measured as a percentage reduction in wound size after every 5-day interval.…”

Section: Methodsmentioning

confidence: 99%

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Allium stipitatumExtract Exhibits In Vivo Antibacterial Activity against Methicillin-ResistantStaphylococcus aureusand Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model

Karunanidhi

Ghaznavi‐Rad

Nathan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs (Allium stipitatum) were explored in a mice burn model infected with methicillin-resistant Staphylococcus aureus (MRSA). Hexane (ASHE) and dichloromethane (ASDE) extracts were tested. Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA. ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days. Silver sulfadiazine (1%) served as drug positive control. Microbiological analysis was carried out on 1, 2, 3, 4, and 5 days postwounding (dpw) and histopathological analysis at the end of the experiment (20 dpw). Both ointments demonstrated strong antibacterial activity with complete elimination of MRSA at 48–72 h after infection. The rate of wound contraction was higher (95–100%) in mice groups treated with ASHE and ASDE ointments after 15 dpw. Histological analysis revealed significant increase (p < 0.05) in epithelialization and collagenation in treated groups. The ASHE and ASDE were found to be relatively noncytotoxic and safe to Vero cell line (383.4 μg mL−1; 390.6 μg mL−1), suggesting the extracts as safe topical antibacterial as well as promising alternatives in managing thermal injuries.

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Section: Discussionmentioning

confidence: 99%

“…Thermal injury procedure was introduced according to the method described previously [23]. Briefly, the dorsal surface of the mice was shaved (2 × 3 cm) one day prior to the experiment.…”

Section: Methodsmentioning

confidence: 99%

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Allium stipitatumExtract Exhibits In Vivo Antibacterial Activity against Methicillin-ResistantStaphylococcus aureusand Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model

Karunanidhi

Ghaznavi‐Rad

Nathan

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Furthermore, these antimicrobials are not effective because bacteria in biofilms can be up to 1,000-fold less sensitive to antibiotics than their planktonic counterparts (10)(11)(12). Other topical disinfectants, such as chlorhexidine (13), silver sulfadiazine (14), and iodine preparations (15), can be very painful when applied to open wounds (16), and the scientific evidence for the efficacy of these agents in wounds is scarce. Clearly, there is an urgent need for novel antimicrobial agents that can be applied topically to (i) prevent colonization and (ii) eliminate infectious agents in burn wounds (17).…”

mentioning

confidence: 99%

LL-37-Derived Peptides Eradicate Multidrug-Resistant Staphylococcus aureus from Thermally Wounded Human Skin Equivalents

Haisma

Breij

Chan

et al. 2014

Antimicrob Agents Chemother

115

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Burn wound infections are often difficult to treat due to the presence of multidrug-resistant bacterial strains and biofilms. Currently, mupirocin is used to eradicate methicillin-resistant Staphylococcus aureus (MRSA) from colonized persons; however, mupirocin resistance is also emerging. Since we consider antimicrobial peptides to be promising candidates for the development of novel anti-infective agents, we studied the antibacterial activities of a set of synthetic peptides against different strains of S. aureus, including mupirocin-resistant MRSA strains. The peptides were derived from P60.4Ac, a peptide based on the human cathelicidin LL-37. The results showed that peptide 10 (P10) was the only peptide more efficient than P60.4Ac, which is better than LL-37, in killing MRSA strain LUH14616. All three peptides displayed good antibiofilm activities. However, both P10 and P60.4Ac were more efficient than LL-37 in eliminating biofilm-associated bacteria. No toxic effects of these three peptides on human epidermal models were detected, as observed morphologically and by staining for mitochondrial activity. In addition, P60.4Ac and P10, but not LL-37, eradicated MRSA LUH14616 and the mupirocin-resistant MRSA strain LUH15051 from thermally wounded human skin equivalents (HSE). Interestingly, P60.4Ac and P10, but not mupirocin, eradicated LUH15051 from the HSEs. None of the peptides affected the excretion of interleukin 8 (IL-8) by thermally wounded HSEs upon MRSA exposure. In conclusion, the synthetic peptides P60.4Ac and P10 appear to be attractive candidates for the development of novel local therapies to treat patients with burn wounds infected with multidrug-resistant bacteria.

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“…Dressings containing NCS have been shown to reduce the systemic spread of MRSA infection in a full‐thickness rat burn wound model, with a similar effect in limiting the spread of both fungal infection and A. baumannii infections into the muscle in these animal models. Furthermore, patients at risk of delayed healing and untreated local infection could progress to systemic sepsis.…”

Section: How Can Dressings Containing Ncs Be Incorporated Into Clinicmentioning

confidence: 94%

Appropriate use of dressings containing nanocrystalline silver to support antimicrobial stewardship in wounds

Woodmansey

Roberts²

2018

International Wound Journal

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Antimicrobial resistance is an ever-increasing global concern, with the era of untreatable infection becoming a reality. Wound care is no exception, with increasing issues of antibiotic-resistant infections across different wound types and care settings. Antibiotic resistance and stewardship have been the priority for most strategic interventions so far; however, in wound care, alternative or supplementary strategies using antiseptics should be considered. Antiseptics such as silver can provide effective cidal activity across a broad range of wound pathogens, assuming they are used at the correct level for an appropriate duration. Evidence summarised in this manuscript suggests that effective antiseptics, such as nanocrystalline silver, have an increasing body of evidence in support of their use to minimise transmission of antibiotic-resistant organisms as part of institutional infection control procedures and, in addition, through appropriate early use and stewardship on local wound infections, in conjunction with local procedures, to minimise the need for systemic antibiotic therapy. Engagement, alignment, and collaboration between wound care professionals and wider related teams and governments on antimicrobial stewardship, and the potential role of antiseptics within this, will help to generate further evidence for such interventions in the fight against antimicrobial-resistant infections in wound care.

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Comparison of the antibacterial effect of silver sulfadiazine 1%, mupirocin 2%, Acticoat and octenidine dihydrochloride in a full-thickness rat burn model contaminated with multi drug resistant Acinetobacter baumannii

Cited by 21 publications

References 27 publications

Allium stipitatumExtract Exhibits In Vivo Antibacterial Activity against Methicillin-ResistantStaphylococcus aureusand Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model

Allium stipitatumExtract Exhibits In Vivo Antibacterial Activity against Methicillin-ResistantStaphylococcus aureusand Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model

LL-37-Derived Peptides Eradicate Multidrug-Resistant Staphylococcus aureus from Thermally Wounded Human Skin Equivalents

Appropriate use of dressings containing nanocrystalline silver to support antimicrobial stewardship in wounds

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