Comparison of the anion inhibition profiles of the β- and γ-carbonic anhydrases from the pathogenic bacterium Burkholderia pseudomallei

Prete, Sonia Del; Vullo, Daniela; Fonzo, Pietro Di; Carginale, Vincenzo; Supuran, Claudiu T.; Capasso, Clemente

doi:10.1016/j.bmc.2017.02.032

Cited by 8 publications

(5 citation statements)

References 78 publications

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“…Our studies have revealed that the ␤-CA protein has, in some cases, potentially evolved into a virulence factor for some pathogenic bacteria, such as Burkholderia pseudomallei (49). We have identified a significant number of ␤-CA genes that reside in GIs in bacterial species, many of which are known to be pathogenic.…”

Section: ␤-Carbonic Anhydrase Genes In Genomic Islandsmentioning

confidence: 99%

Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists

Emameh

Barker

Hytönen

et al. 2018

Appl Environ Microbiol

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Genomic islands (GIs) are a type of mobile genetic element (MGE) that are present in bacterial chromosomes. They consist of a cluster of genes that produce proteins that contribute to a variety of functions, including, but not limited to, the regulation of cell metabolism, antimicrobial resistance, pathogenicity, virulence, and resistance to heavy metals. The genes carried in MGEs can be used as a trait reservoir in times of adversity. Transfer of genes using MGEs, occurring outside reproduction, is called horizontal gene transfer (HGT). Previous data have shown that numerous HGT events have occurred through endosymbiosis between prokaryotes and eukaryotes. β-Carbonic anhydrase (β-CA) enzymes play a critical role in the biochemical pathways of many prokaryotes and eukaryotes. We previously suggested the horizontal transfer of β-CA genes from plasmids of some prokaryotic endosymbionts to their protozoan hosts. In this study, we set out to identify β-CA genes that might have been transferred between prokaryotic and protist species through HGT in GIs. Therefore, we investigated prokaryotic chromosomes containing β-CA-encoding GIs and utilized multiple bioinformatics tools to reveal the distinct movements of β-CA genes among a wide variety of organisms. Our results identify the presence of β-CA genes in GIs of several medically and industrially relevant bacterial species, and phylogenetic analyses reveal multiple cases of likely horizontal transfer of β-CA genes from GIs of ancestral prokaryotes to protists. The evolutionary process is mediated by mobile genetic elements (MGEs), such as genomic islands (GIs). A gene or set of genes in the GIs is exchanged between and within various species through horizontal gene transfer (HGT). Based on the crucial role that GIs can play in bacterial survival and proliferation, they were introduced as environment- and pathogen-associated factors. Carbonic anhydrases (CAs) are involved in many critical biochemical pathways, such as the regulation of pH homeostasis and electrolyte transfer. Among the six evolutionary families of CAs, β-CA gene sequences are present in many bacterial species, which can be horizontally transferred to protists during evolution. This study shows the involvement of bacterial β-CA gene sequences in the GIs and suggests their horizontal transfer to protists during evolution.

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Section: ␤-Carbonic Anhydrase Genes In Genomic Islandsmentioning

confidence: 99%

Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists

Emameh

Barker

Hytönen

et al. 2018

Appl Environ Microbiol

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“…Within a general research project aimed at developing new anti-melioidosis drugs, we have undertaken a detailed characterization of these two enzymes. In particular, both enzymes were produced in Escherichia coli and kinetically characterized, showing to be moderately active in catalyzing the CO 2 hydration reaction (k cat = 1.6·10 5 s −1 , k cat /K m = 3.4·10 7 M −1 s −1 for BpsβCA and k cat = 5.3·10 5 s −1 , k cat /K m = 2.5·10 7 M −1 s −1 for BpsγCA) [38] , [39] , [40] ; furthermore their inhibition profile with different classes of molecules was deeply investigated [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] . However, while the crystallographic structure of BpsβCA was determined [38] , to date any structural information on BpsγCA is missing.…”

Section: Introductionmentioning

confidence: 99%

Biochemical, structural, and computational studies of a γ-carbonic anhydrase from the pathogenic bacterium Burkholderia pseudomallei

Fiore

Luca²,

Langella³

et al. 2022

Computational and Structural Biotechnology Journal

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“…Whereas bacterial CA inhibitors (CAIs) were extensively studied, leading to a detailed understanding of the catalytic and inhibition mechanisms, only a few studies are available on the bacterial CAAs. Recently, our groups described the biochemical properties of a β- and γ-CA from the pathogenic bacterium Burkholderia pseudomallei , which is responsible for the tropical disease melioidosis 48–51 . These enzymes, called BpsCAβ and BpsCAγ, showed high catalytic activity for the physiologic CO 2 hydration reaction to bicarbonate and protons ( k cat 10 5 s −1 ) 48–51 .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, our groups described the biochemical properties of a β- and γ-CA from the pathogenic bacterium Burkholderia pseudomallei , which is responsible for the tropical disease melioidosis 48–51 . These enzymes, called BpsCAβ and BpsCAγ, showed high catalytic activity for the physiologic CO 2 hydration reaction to bicarbonate and protons ( k cat 10 5 s −1 ) 48–51 . Moreover, the study of the inhibition profiles with the classical CA inhibitors (sulfonamides and anions) revealed an interesting structure–activity relationship for the interaction of these enzymes with the inhibitors 48–51 .…”

Section: Introductionmentioning

confidence: 99%

“…These enzymes, called BpsCAβ and BpsCAγ, showed high catalytic activity for the physiologic CO 2 hydration reaction to bicarbonate and protons ( k cat 10 5 s −1 ) 48–51 . Moreover, the study of the inhibition profiles with the classical CA inhibitors (sulfonamides and anions) revealed an interesting structure–activity relationship for the interaction of these enzymes with the inhibitors 48–51 . We also investigated the activation profiles of BpsCAγ with a series of natural and non-natural amino acids and aromatic/heterocyclic amines 52 .…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the amine/amino acid activation profiles of the β- and γ-carbonic anhydrases from the pathogenic bacteriumBurkholderia pseudomallei

Vullo

Prete

Osman

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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The β-class carbonic anhydrase (CA, EC 4.2.1.1) from the pathogenic bacterium Burkholderia pseudomallei, BpsCAβ, that is responsible for the tropical disease melioidosis was investigated for its activation with natural and non-natural amino acids and amines. Previously, the γ-CA from this bacterium has been investigated with the same library of 19 amines/amino acids, which show very potent activating effects on both enzymes. The most effective BpsCAβ activators were L- and D-DOPA, L- and D-Trp, L-Tyr, 4-amino-L-Phe, histamine, dopamine, serotonin, 2-pyridyl-methylamine, 1-(2-aminoethyl)-piperazine and L-adrenaline with KAs of 0.9–27 nM. Less effective activators were D-His, L- and D-Phe, D-Tyr, 2-(2-aminoethyl)pyridine and 4-(2-aminoethyl)-morpholine with KAs of 73 nM–3.42 µM. The activation of CAs from bacteria, such as BpsCAγ/β, has not been considered previously for possible biomedical applications. It would be of interest to perform studies in which bacteria are cultivated in the presence of CA activators, which may contribute to understanding processes connected with the virulence and colonization of the host by pathogenic bacteria.

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Comparison of the anion inhibition profiles of the β- and γ-carbonic anhydrases from the pathogenic bacterium Burkholderia pseudomallei

Cited by 8 publications

References 78 publications

Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists

Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists

Biochemical, structural, and computational studies of a γ-carbonic anhydrase from the pathogenic bacterium Burkholderia pseudomallei

Comparison of the amine/amino acid activation profiles of the β- and γ-carbonic anhydrases from the pathogenic bacteriumBurkholderia pseudomallei

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