Comparison of the Angiogenic Ability between SHED and DPSC in a Mice Model with Critical Limb Ischemic

Zhou, Yong; Kuang, Gu; Sun, Fubao; Shoumei, Xuan; Zou, Duohong; He, Jiacai; Tang, Xu-yan

doi:10.1007/s13770-022-00452-6

Cited by 6 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DPSCs are dental pulp-derived MSCs with strong angiogenic potential (Yong et al 2022). DPSCs promote vascular formation by increasing EC proliferation and migration through secreting VEGF and enhancing the stabilization of newly formed blood vessels by acting as pericyte-like cells (Dissanayaka et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Ang1/Tie2/VE-Cadherin Signaling Regulates DPSCs in Vascular Maturation

Zhang,

Lin,

Liu

et al. 2023

J Dent Res

View full text Add to dashboard Cite

Adding dental pulp stem cells (DPSCs) to vascular endothelial cell–formed vessel-like structures can increase the longevity of these vessel networks. DPSCs display pericyte-like cell functions and closely assemble endothelial cells (ECs). However, the mechanisms of DPSC-derived pericyte-like cells in stabilizing the vessel networks are not fully understood. In this study, we investigated the functions of E-DPSCs, which were DPSCs isolated from the direct coculture of human umbilical vein endothelial cells (HUVECs) and DPSCs, and T-DPSCs, which were DPSCs treated by transforming growth factor beta 1 (TGF-β1), in stabilizing blood vessels in vitro and in vivo. A 3-dimensional coculture spheroid sprouting assay was conducted to compare the functions of E-DPSCs and T-DPSCs in vitro. Dental pulp angiogenesis in the severe combined immunodeficiency (SCID) mouse model was used to explore the roles of E-DPSCs and T-DPSCs in vascularization in vivo. The results demonstrated that both E-DPSCs and T-DPSCs possess smooth muscle cell–like cell properties, exhibiting higher expression of the mural cell–specific markers and the suppression of HUVEC sprouting. E-DPSCs and T-DPSCs inhibited HUVEC sprouting by activating TEK tyrosine kinase (Tie2) signaling, upregulating vascular endothelial (VE)–cadherin, and downregulating vascular endothelial growth factor receptor 2 (VEGFR2). In vivo study revealed more perfused and total blood vessels in the HUVEC + E-DPSC group, HUVEC + T-DPSC group, angiopoietin 1 (Ang1) pretreated group, and vascular endothelial protein tyrosine phosphatase (VE-PTP) inhibitor pretreated group, compared to HUVEC + DPSC group. In conclusion, these data indicated that E-DPSCs and T-DPSCs could stabilize the newly formed blood vessels and accelerate their perfusion. The critical regulating pathways are Ang1/Tie2/VE-cadherin and VEGF/VEGFR2 signaling.

show abstract

Section: Discussionmentioning

confidence: 99%

Ang1/Tie2/VE-Cadherin Signaling Regulates DPSCs in Vascular Maturation

Zhang,

Lin,

Liu

et al. 2023

J Dent Res

View full text Add to dashboard Cite

show abstract

“…The key points in the development of novel tissue engineering therapy are to determine the suitable sources and doses of seed cells needed [58]. For the regeneration of maxillofacial bone defect, PDLSCs, DPSCs and SCAPs could be perfectly efficient candidates and are popularly studied hDMSCs that possess various differential abilities [59][60][61][62][63]. All three hDMSCs have been analyzed for osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

Efficient bone regeneration of BMP9-stimulated human periodontal ligament stem cells (hPDLSCs) in decellularized bone matrix (DBM) constructs to model maxillofacial intrabony defect repair

et al. 2022

View full text Add to dashboard Cite

Background BMP9-stimulated DPSCs, SCAPs and PDLSCs are effective candidates for repairing maxillofacial bone defects in tissue engineering, while the most suitable seed cell source among these three hDMSCs and the optimal combination of most suitable type of hDMSCs and BMP9 have rarely been explored. Moreover, the orthotopic maxillofacial bone defect model should be valuable but laborious and time-consuming to evaluate various candidates for bone regeneration. Thus, inspired from the maxillofacial bone defects and the traditional in vivo ectopic systems, we developed an intrabony defect repair model to recapitulate the healing events of orthotopic maxillofacial bone defect repair and further explore the optimized combinations of most suitable hDMSCs and BMP9 for bone defect repair based on this modified ectopic system. Methods Intrabony defect repair model was developed by using decellularized bone matrix (DBM) constructs prepared from the cancellous part of porcine lumbar vertebral body. We implanted DBM constructs subcutaneously on the flank of each male NU/NU athymic nude mouse, followed by directly injecting the cell suspension of different combinations of hDMSCs and BMP9 into the central hollow area of the constructs 7 days later. Then, the quality of the bony mass, including bone volume fraction (BV/TV), radiographic density (in Hounsfield units (HU)) and the height of newly formed bone, was measured by micro-CT. Furthermore, the H&E staining and immunohistochemical staining were performed to exam new bone and new blood vessel formation in DBM constructs. Results BMP9-stimulated periodontal ligament stem cells (PDLSCs) exhibited the most effective bone regeneration among the three types of hDMSCs in DBM constructs. Furthermore, an optimal dose of PDLSCs with a specific extent of BMP9 stimulation was confirmed for efficacious new bone and new blood vessel formation in DBM constructs. Conclusions The reported intrabony defect repair model can be used to identify optimized combinations of suitable seed cells and biological factors for bone defect repair and subsequent development of efficacious bone tissue engineering therapies.

show abstract

“…DPSCs 24,25 Tissue specificity Available pulp tissue is reduced under pathological conditions like pulpitis and apical periodontitis Potent capacity Easy surgical availability Ability to form loose connective tissue similar to native pulp tissue SHEDs 26,27 Tissue specificity…”

Section: Stem Cells Advantages Disadvantagesmentioning

confidence: 99%