Comparison of the analgesic effects between electro-acupuncture and moxibustion with visceral hypersensitivity rats in irritable bowel syndrome

Abstract

AIMTo observe whether there are differences in the effects of electro-acupuncture (EA) and moxibustion (Mox) in rats with visceral hypersensitivity.METHODSEA at 1 mA and 3 mA and Mox at 43 °C and 46 °C were applied to the Shangjuxu (ST37, bilateral) acupoints in model rats with visceral hypersensitivity. Responses of wide dynamic range neurons in dorsal horns of the spinal cord were observed through the extracellular recordings. Mast cells (MC) activity in the colons of rats were assessed, and 5-hydroxytryptamine (5-HT), 5-hydroxytryptamine 3 receptor (5-HT3R) and 5-HT4R expressions in the colons were measured.RESULTSCompared with normal control group, responses of wide dynamic range neurons in the dorsal horn of the spinal cord were increased in the EA at 1 mA and 3 mA groups (1 mA: 0.84 ± 0.74 vs 2.73 ± 0.65, P < 0.001; 3 mA: 1.91 ± 1.48 vs 6.44 ± 1.26, P < 0.001) and Mox at 43 °C and 46 °C groups (43 °C: 1.76 ± 0.81 vs 4.14 ± 1.83, P = 0.001; 46 °C: 5.19 ± 2.03 vs 7.91 ± 2.27, P = 0.01). MC degranulation rates and the expression of 5-HT, 5-HT3R and 5-HT4R in the colon of Mox 46 °C group were decreased compared with model group (MC degranulation rates: 0.47 ± 0.56 vs 0.28 ± 0.78, P < 0.001; 5-HT: 1.42 ± 0.65 vs 7.38 ± 1.12, P < 0.001; 5-HT3R: 6.62 ± 0.77 vs 2.86 ± 0.88, P < 0.001; 5-HT4R: 4.62 ± 0.65 vs 2.22 ± 0.97, P < 0.001).CONCLUSIONThe analgesic effects of Mox at 46 °C are greater than those of Mox at 43 °C, EA 1 mA and EA 3 mA.

Electroacupuncture Inhibits the Activity of Astrocytes in Spinal Cord in Rats with Visceral Hypersensitivity by Inhibiting P2Y1 Receptor-Mediated MAPK/ERK Signaling Pathway

“…Acetic acid induced visceral hypersensitivity in normal rats which lasted till week 7. e Evidence-Based Complementary and Alternative Medicine Evidence-Based Complementary and Alternative Medicine behavior of experimental animals was found to be stable which is in line with the behavioral characteristics of visceral hypersensitivity in IBS. A large number of studies have reported the positive effect of EA in the treatment of IBS visceral hypersensitivity [47,48]. In this study, we found that EA at Zusanli and Shangjuxu (100 Hz for 1.05 s, 2 Hz for 2.85 s alternately, pulse width for 0.1 ms, 1 mA, 30 min/d, once a day) significantly reduced the behavioral AWR score of IBS rats suggesting that EA significantly relieved the visceral hypersensitivity in our IBS rat.…”

Pharmacological basis for application of scutellarin in Alzheimer's disease: Antioxidation and antiapoptosis

“…Of the 42 mice, 30 were subcutaneously injected 120 mg/kg D-gal and orally treated with 20 mg/kg AlCl 3 once per day for 8 weeks to establish an AD mice model similar to a previous study ( 16 ). At the fifth week, only 24 mice were retained for further experimentation, as the remaining 6 mice failed to exhibit AD-associated behaviors in the water maze test, and these 24 mince were then randomly divided into two groups, which were either treated orally with normal saline solution (n=12) or 20 mg/kg of SC (n=12) for 4 weeks.…”

“…Normal mice (n=12) treated orally with normal saline solution for 8 weeks, served as the control group. From week 9, behavioral tests, including autonomic activity and a Morris water maze tests, were performed according to a previous study without modifications ( 16 ). Orbital blood (300 µl) was sampled via peritoneal injection following anesthesia with 400 mg/kg of 10% chloral hydrate.…”

PDTC Alleviates Depressive Symptoms and Colon Tissue Injury via Inhibiting NO Overproduction in CUMS Rats

“…Tissue preparation of colon and spinal cord: A segment of proximal colon (1 cm from caecum) and spinal cord at L1-2 tissues were collected (Zhao et al, 2017) and then stored at −80 • C until assayed. First, the frozen tissues were homogenized in cytoplasmic extraction reagent A CERA (0.2 ml) (BioTeke Corporation, Beijing, China).…”